Abstract：
:In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-β-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis for glutamate dehydrogenase (gdh), phosphoglucomutase (pgm) and esterase (est) metabolic genes, in comparison to RNA polymerase β subunit (rpoB) and 16S ribosomal RNA (rrs) gene sequencing, to identify the presence of these two groups in seventeen B. fragilis strains. Based on phylogenetic trees, only the est gene sequences generated a classification similar to rrs- and rpoB-genes. On the other hand, the genes pgm and gdh did not allow the discrimination of these divisions. The est gene sequence can be suggested as an additional tool for differentiation of the two groups in B. fragilis, providing highly reproducible and reliable data in B. fragilis taxonomy.
journal_name
Anaerobejournal_title
Anaerobeauthors
Miranda KR,Neves FP,Santos-Filho Jd,de Paula GR,Lobo LA,Oelemann WM,Domingues RMdoi
10.1016/j.anaerobe.2012.11.005subject
Has Abstractpub_date
2013-02-01 00:00:00pages
58-61eissn
1075-9964issn
1095-8274pii
S1075-9964(12)00154-0journal_volume
19pub_type
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