Abstract：
:Members of Dysgonomonas are Gram-stain-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation from stool and wounds of human patients (CDC group DF-3). More recently, Dysgonomonas have been found to be widely distributed in terrestrial environments and are particularly enriched in insect systems. Their prevalence in xylophagous insects such as termites and wood-feeding cockroaches, as well as in soil-fed microbial fuel cells, elicit interest in lignocellulose degradation and biofuel production, respectively. Their occurrence in mosquito and fruit fly have implications relating to symbiosis, host immunology and developmental biology. Additionally, their presence in termite, mosquito and nematode present novel opportunities for pest and vector control. Currently, the absolute growth requirements of Dysgonomonas are unknown, and they are commonly cultured under anaerobic conditions on complex media containing blood, peptones, tryptones, and yeast, plant or meat extracts. Restrictive and undefined culturing conditions preclude physiological and genetic studies, and thus further understanding of their metabolic potential. Here we describe the requirements for growth of termite-derived Dysgonomonas isolates and create parallel complex, defined and minimal media that permit vigorous and reliable aerobic growth. Furthermore, we show that these media can be used to easily enrich for Dysgonomonas isolates from densely-colonized and microbially-diverse environmental samples.
journal_name
Anaerobejournal_title
Anaerobeauthors
Bridges CM,Gage DJdoi
10.1016/j.anaerobe.2020.102302subject
Has Abstractpub_date
2020-12-01 00:00:00pages
102302eissn
1075-9964issn
1095-8274pii
S1075-9964(20)30158-Xjournal_volume
67pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1998.0180
更新日期：1999-02-01 00:00:00
abstract：:The chemostat model has been an important tool in studying intestinal microflora. To date, several competitive exclusion products have been developed from such studies as prophylactic treatment against pathogenic bacteria. A continuous-flow chemostat model of a feral pig was developed using inocula from the cecal cont...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.03.003
更新日期：2007-06-01 00:00:00
abstract：:The aim of the study was to purify the exopolysaccharides (EPS) produced from Bifidobacterium animalis RH, which was isolated from the feces of Bama centenarians in Guangxi of China, and evaluate their antioxidant activities in vitro and in vivo. 2 fractions, a neutral EPS fraction (EPSa) and an acidic EPS fraction (E...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.07.010
更新日期：2011-10-01 00:00:00
abstract：:Quorum sensing (QS) signaling regulates the motility, adhesion, and biofilm formation of bacteria, and at the same time activates immune response in eukaryotic organisms. We recently demonstrated that the QS molecule, dihydroxy-2, 3-pentanedione (DPD), and its analogs significantly inhibit estradiol-regulated virulenc...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.09.001
更新日期：2018-12-01 00:00:00
abstract：:In the present research, the 20 lactobacilli isolated from children feces aged 4-15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected ant...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.09.006
更新日期：2013-12-01 00:00:00
abstract：:Adherence of Bilophila wadsworthia to the cultured human embryonic intestinal cell line, Intestine 407 (Int 407), varied among the strains tested from strongly adherent (76-100% cells positive for one or more adherent bacteria) to non- or weakly adherent (0-25% positive cells). Although negative staining revealed that...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0134
更新日期：1998-02-01 00:00:00
abstract：:Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, r...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.06.006
更新日期：2010-08-01 00:00:00
abstract：:Clostridium difficile causes diarrhoeal diseases ranging from asymptomatic carriage to a fulminant, relapsing, and potentially fatal colitis. Endospore production plays a vital role in transmission of infection, and in order to cause disease these spores must then germinate and return to vegetative cell growth. Type B...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:Ice used for direct human consumption or to preserve foods and cool down drinks can be contaminated with pathogenic microorganisms and may potentially become a vehicle for consumer's infection. To evaluate physical, chemical and microbiological quality of commercial ice and ice used for fish and seafood, 100 ice sampl...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.06.005
更新日期：2011-12-01 00:00:00
abstract：:The ultrastructural colony architecture of six Clostridium difficile strains belonging to four different ribotypes (001, 027, 078/126 and 036) was studied by scanning electron microscopy (SEM). In 2-day-old colonies several microareas differing in cell length and organization could be differentiated. Some cells at col...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.09.014
更新日期：2013-12-01 00:00:00
abstract：:Immuno-cross reactivity between the subunits of Clostridium thermocellum cellulosome and cloned endogucanases and xylanase from the same bacterium was studied using the polyclonal antibodies raised against cloned enzymes. Dot blot analysis showed that the cellulosome, S8 and S11 subunits cross-reacted strongly with th...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0119
更新日期：1997-10-01 00:00:00
abstract：:Clostridium spp. are recovered from 25% of the blood culture positive with anaerobes. However, the clinical relevance of Clostridium bacteremia has been controverted in the literature, particularly for C. perfringens. We aimed to evaluate the clinical relevance of Clostridium bacteremia, either due to C. perfringens o...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102202
更新日期：2020-06-01 00:00:00
abstract：:Specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. Only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria i...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1995.1031
更新日期：1995-12-01 00:00:00
abstract：:Post-sternotomy mediastinitis, a nosocomial infection mostly caused by staphylococci, can be life-threatening. A case of mediastinitis due to Finegoldia magna after a coronary artery bypass graft surgery was reviewed. Although this bacterium is difficult to be isolated from routine blood cultures, a F. magna bacteriem...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.11.012
更新日期：2015-04-01 00:00:00
abstract：:This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin bindi...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2016.10.007
更新日期：2016-12-01 00:00:00
abstract：:Diagnostic laboratories are urged to take advantage of novel technological advancements to provide standardized and high-throughput information for clinicians; however, total laboratory automation (TLA) has only recently been introduced in clinical microbiology in the last 10-12 years. The introduction of total labora...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.06.009
更新日期：2019-10-01 00:00:00
abstract：:The members of the phylum Fusobacteria and its two families, Fusobacteriaceae and Leptotrichiaceae, are distinguished at present mainly on the basis of their branching in the 16S rRNA gene trees and analysis of the internal transcribed spacer sequences in the 16S-23S rDNA. However, no biochemical or molecular characte...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.06.007
更新日期：2014-08-01 00:00:00
abstract：:Dental plaque is a multispecies biofilm in the oral cavity that significantly influences oral health. The presence of the oral anaerobic pathogen Porphyromonas gingivalis is an important determinant in the development of periodontitis. Direct and indirect interactions between P. gingivalis and the host play a major ro...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.11.011
更新日期：2015-10-01 00:00:00
abstract：:Lacto-N-biose I (LNB) is a potential factor for the selective growth of bifidobacteria. We previously reported that the species of bifidobacteria predominant in infant intestines might use LNB. We aimed to assess the prebiotic properties of LNB in comparison to other oligosaccharides using an in vitro fermentation sys...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.12.007
更新日期：2013-02-01 00:00:00
abstract：:Cellulase family and some other glycosyl hydrolases of anaerobic fungi inhabiting the digestive tract of ruminants are believed to form an enzyme complex called cellulosome. Study of the individual component of cellulosome may shed light on understanding the organization of this complex and its functional mechanism. W...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/S1075-9964(03)00087-8
更新日期：2003-06-01 00:00:00
abstract：:Proteins play a pivotal role in thermophily. Comparing the molecular properties of homologous proteins from thermophilic and mesophilic bacteria is important for understanding the mechanisms of microbial adaptation to extreme environments. The thermophile Thermoanaerobacter (Thermoanaerobium) brockii and the mesophile...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0083
更新日期：1997-08-01 00:00:00
abstract：:With the emergence of antibiotic resistance among Bacteroides fragilis group isolates the need of susceptibility testing in routine laboratories is increasing. The aims of the present study were to evaluate the disk diffusion method for susceptibility testing in case of different clinical isolates of Bacteroides spp b...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.10.008
更新日期：2015-02-01 00:00:00
abstract：:Clostridioides difficile is a Gram-positive, anaerobic bacterium. It is known that C. difficile is one of the major causes of antibiotic associated diarrhea. The enhanced antibiotic resistance observed in C. difficile is the result of highly resistant spores produced by the bacterium. In Bacillus subtilis, the sin ope...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.05.002
更新日期：2019-10-01 00:00:00
abstract：:Azo dyes are ubiquitous in products and often become environmental pollutants due to their anthropogenic nature. Azoreductases are enzymes which are present within many bacteria and are capable of breaking down the azo dyes via reduction of the azo bond. Often, though, carcinogenic aromatic amines are formed as metabo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.07.005
更新日期：2013-10-01 00:00:00
abstract：:Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-char...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.08.014
更新日期：2017-12-01 00:00:00
abstract：:Several studies have suggested the association of disturbed genital tract microbiota with infertility. Our aim was to clarify the influence of sexual intercourse on partner's genital tract microbiota in infertile couples. Seventeen couples were studied, and in 5 men inflammatory prostatitis (IP) was diagnosed. Semen s...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.04.015
更新日期：2011-12-01 00:00:00
abstract：:The mammalian gastrointestinal tract harbors a vast microbial ecosystem, known as the microbiota, which benefits host biology. Bacteroides fragilis is an important anaerobic gut commensal of humans that prevents and cures intestinal inflammation. We wished to elucidate aspects of gut colonization employed by B. fragil...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.05.017
更新日期：2011-08-01 00:00:00
abstract：:The gastrointestinal tract is a balanced ecosystem that can get out of balance and predisposed to clostridial diseases or other pathological conditions. The objective of the present study was to evaluate the gut microbiota in dairy cows suffering from chronic botulism. Cows were investigated for Clostridium (C.) botul...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.03.013
更新日期：2014-06-01 00:00:00
abstract：:Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rate disease affecting mainly cattle and sheep. Carcasses of animals affected by the disease are the chief source of soil infection and considered as an ever-present threat to livestock health. A study was undertaken to examine the cross-contami...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.01.004
更新日期：2008-06-01 00:00:00
abstract：:Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.12.009
更新日期：2018-02-01 00:00:00
