Abstract：
:Immuno-cross reactivity between the subunits of Clostridium thermocellum cellulosome and cloned endogucanases and xylanase from the same bacterium was studied using the polyclonal antibodies raised against cloned enzymes. Dot blot analysis showed that the cellulosome, S8 and S11 subunits cross-reacted strongly with the antibodies of all cloned enzymes tested except that raised against CelC. Western blot analysis revealed that S8 and S11 subunits cross-reacted with the antibodies of CelA, CelB, CelD, CelG, CelH and XynZ, but the antibodies of CelB and CelG were highly specific for S8 and S11 subunits, respectively. Similar analysis using dissociated cellulosome showed that the antibodies of all cloned enzymes tested cross-reacted with more than one subunit of the cellulosome. Antibodies of CelC showed a very low cross-reactivity against all subunits of the cellulosome. The results indicate that immunological cross-reactivity studies could be useful, not only for demonstrating the similarities between native and cloned enzymes, but also for identifying native enzymes using antibodies of cloned enzymes.
journal_name
Anaerobejournal_title
Anaerobeauthors
Bhat S,Hutson RA,Owen E,Bhat MKdoi
10.1006/anae.1997.0119subject
Has Abstractpub_date
1997-10-01 00:00:00pages
347-52issue
5eissn
1075-9964issn
1095-8274pii
S1075-9964(97)90119-0journal_volume
3pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:There is concern that the bacterial colony counts present at the time of manufacture and listed on the probiotic package may not be reflective of the numbers viable colonies at the time of purchase and patient consumption thereby diminishing efficacy. We performed a colony count study of three separate samples of five...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：:Prevotella dentalis is a Gram-negative anaerobic rod involved in various human diseases, especially oral infections. We report a rare case of a pleural effusion due to this microorganism in an elderly patient. An 88-year-old man with chronic respiratory disease presented with a left pleural effusion for more than 1 mo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.09.004
更新日期：2018-12-01 00:00:00
abstract：:Eggerthella lenta is increasingly found in patients with severe comorbidities. Because oncologic patients are exposed to emerging pathogens, we aimed to describe the factors associated with E. lenta bacteremia in this immunosuppressed population. Oncology patients with blood cultures positive for E. lenta were retrosp...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.04.010
更新日期：2017-10-01 00:00:00
abstract：:In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-β-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis fo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.11.005
更新日期：2013-02-01 00:00:00
abstract：:The aim of this work was to investigate the possible role of the intestinal anaerobic flora (especially bifidobacteria) in regulating bacterial translocation (BT) which can be defined as the passage of intestinal microbes through the mucosa to internal organs. Default in BT regulation concurs with pathogenesis of seps...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.09.003
更新日期：2008-02-01 00:00:00
abstract：:Clostridium spp. are recovered from 25% of the blood culture positive with anaerobes. However, the clinical relevance of Clostridium bacteremia has been controverted in the literature, particularly for C. perfringens. We aimed to evaluate the clinical relevance of Clostridium bacteremia, either due to C. perfringens o...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102202
更新日期：2020-06-01 00:00:00
abstract：:Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1998.0180
更新日期：1999-02-01 00:00:00
abstract：:Clostridium perfringens isolates associated with food poisoning carries a chromosomal cpe gene, while non-foodborne human gastrointestinal disease isolates carry a plasmid cpe gene. The enterotoxigenic strains tested produced vegetative cells and spores with significantly higher resistance than non-enterotoxigenic str...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.03.007
更新日期：2016-06-01 00:00:00
abstract：:Bacteroides thetaiotaomicron maybe one of the most adaptable intestinal bacteria due to its complex genome. Known to be an opportunistic pathogenic anaerobe, B. thetaiotaomicron has recently been described as a symbiont with anti-inflammatory properties. In this study, peptide mass finger printing technique was used t...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.04.008
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Mammalian gut microbiota have been implicated in a variety of functions including the breakdown of ingested nutrients, the regulation of energy intake and storage, the control of immune system development and activity, and the synthesis of novel chemicals. Previous studies have shown that feeding mammalian h...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.02.004
更新日期：2012-06-01 00:00:00
abstract：:Here, we sought to assess the levels of antibiotic resistance among intestinal Bacteroides and Parabacteroides strains collected between 2014 and 2016 in Europe and also attempted to compare resistance levels between clinical and commensal isolates. Bacteroides and Parabacteroides isolates were recovered from faecal s...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102182
更新日期：2020-04-01 00:00:00
abstract：:Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria P...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.05.013
更新日期：2018-08-01 00:00:00
abstract：:In this paper, the bioactivity of cell-free extracts obtained by dairy propionibacteria strains was investigated. Probiotic bifidobacteria and lactobacilli were used as microbial targets. The extracellular filtrates were added as ingredient (1% v/v) into the growth medium; the effect of cell-free filtrates was evaluat...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.09.016
更新日期：2014-12-01 00:00:00
abstract：:Clostridium difficile is a Gram-positive spore forming anaerobic bacterium and the main cause of healthcare-associated diarrhea. This study aimed to perform the phenotypic characterization and molecular typing of Clostridium difficile isolates among patients at a cancer hospital in Brazil. During 18 months, 48 diarrhe...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.10.001
更新日期：2017-12-01 00:00:00
abstract：:Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the only efficient strategy in order to control the d...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.02.011
更新日期：2019-04-01 00:00:00
abstract：:The aim of the study was to purify the exopolysaccharides (EPS) produced from Bifidobacterium animalis RH, which was isolated from the feces of Bama centenarians in Guangxi of China, and evaluate their antioxidant activities in vitro and in vivo. 2 fractions, a neutral EPS fraction (EPSa) and an acidic EPS fraction (E...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.07.010
更新日期：2011-10-01 00:00:00
abstract：:Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.04.001
更新日期：2017-06-01 00:00:00
abstract：:Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the colonizing Bifidobacterium species influences the prevalence of allergic diseases. This...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.05.006
更新日期：2014-08-01 00:00:00
abstract：:Due to increasing antibiotic resistance among anaerobic bacteria, routine antimicrobial susceptibility testing is recommended by the Clinical and Laboratory Standards Institute (CLSI). This study compared the minimum inhibitory concentrations (MICs) from 920 Clostridium difficile isolates tested against seven antimicr...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.02.006
更新日期：2017-04-01 00:00:00
abstract：:To investigate whether there is a nexus between the microbial diversity level (taxonomic, functional and ecological) and the stress tolerance potential of the microbial community, the impact of different ammonium sources was evaluated. Therefore reactors adapted either to the anaerobic digestions of sugar beet silage ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.01.003
更新日期：2019-04-01 00:00:00
abstract：:Two bacterial 16S rRNA gene clone libraries were constructed from the forestomach of alpacas and sheep fed alfalfa. After the amplification using the universal 16S rRNA gene primers, equal quantities of PCR products from the same species were mixed and used to construct the two libraries. Sequence analysis showed that...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.06.004
更新日期：2010-08-01 00:00:00
abstract：:Proteins play a pivotal role in thermophily. Comparing the molecular properties of homologous proteins from thermophilic and mesophilic bacteria is important for understanding the mechanisms of microbial adaptation to extreme environments. The thermophile Thermoanaerobacter (Thermoanaerobium) brockii and the mesophile...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0083
更新日期：1997-08-01 00:00:00
abstract：:As shown previously, a recombinant alphatoxin variant (rAT121A/91) constructed from the naturally occurring Clostridium perfringens mutant strain 121A/91, was devoid of enzymatic (PLC), hemolytic and lethal activity (18). In the present study, the recombinant variant was altered by an oligonucleotide-directed reversio...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.06.003
更新日期：2006-02-01 00:00:00
abstract：:Gut fungal-specific PCR primers have been used to selectively amplify the ITS1 region of gut fungal rDNA recovered from faeces of domestic and wild animals to investigate population diversity. Two different gel-based methods are described for separating populations of gut fungal rDNA amplicons, namely (1) denaturing g...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.05.003
更新日期：2010-04-01 00:00:00
abstract：:Some Australian strain types of Clostridium difficile appear unique, highlighting the global diversity of this bacterium. We examined recent and historic local isolates, finding predominantly toxinotype 0 strains, but also toxinotypes V and VIII. All isolates tested were susceptible to vancomycin and metronidazole, wh...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2015.05.001
更新日期：2015-08-01 00:00:00
abstract：:The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2017.01.001
更新日期：2017-04-01 00:00:00
abstract：:Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillu...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.08.001
更新日期：2012-10-01 00:00:00
abstract：:Since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. For example, reclassification of the 'Bacteroides gracilis group' reve...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.2002.0436
更新日期：2002-10-01 00:00:00
abstract：:The global emergence of epidemic Clostridium difficile PCR ribotype (RT) 027 prompted enhanced surveillance of emerging strains. Recently, there have been reports of severe C. difficile infection in Australia caused by an unusual strain of C. difficile not seen previously. Identified as PCR RT251, this strain produces...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.06.019
更新日期：2019-12-01 00:00:00
abstract：BACKGROUND:The incidence of Clostridium difficile infections (CDI) in the Clinical Center of Serbia (CCS) and the entire Serbia has been constantly rising in the previous 5 years. We aimed to study C. difficile PCR-ribotypes isolated from patients hospitalized at two healthcare institutions: CCS and Specialized Hospita...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.04.005
更新日期：2018-06-01 00:00:00
