Prevalence of CTGCAG recognizing restriction and modification systems in ruminal selenomonades.

Abstract:

:Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains tested. In one strain, the Pst I isoschizomeric restriction system was accompanied by another restriction and modification system recognizing GAATTC sequence (Eco RI isoschizomer). Four other strains contained CTGCAG specific methylases which lacked cognate endo-nuclease activities. Presence of identical restriction and modification systems in both of subspecies of S. ruminantium, as well as the occurrence of Pst I isoschizomers in various combinations, indicate the possibility of horizontal transfer of genes coding for these systems.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Molnarova V,Pristas P,Javorsky P

doi

10.1006/anae.1998.0180

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

37-41

issue

1

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(98)90180-9

journal_volume

5

pub_type

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