Abstract:
:Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains tested. In one strain, the Pst I isoschizomeric restriction system was accompanied by another restriction and modification system recognizing GAATTC sequence (Eco RI isoschizomer). Four other strains contained CTGCAG specific methylases which lacked cognate endo-nuclease activities. Presence of identical restriction and modification systems in both of subspecies of S. ruminantium, as well as the occurrence of Pst I isoschizomers in various combinations, indicate the possibility of horizontal transfer of genes coding for these systems.
journal_name
Anaerobejournal_title
Anaerobeauthors
Molnarova V,Pristas P,Javorsky Pdoi
10.1006/anae.1998.0180subject
Has Abstractpub_date
1999-02-01 00:00:00pages
37-41issue
1eissn
1075-9964issn
1095-8274pii
S1075-9964(98)90180-9journal_volume
5pub_type
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