Abstract：
:Diagnostic laboratories are urged to take advantage of novel technological advancements to provide standardized and high-throughput information for clinicians; however, total laboratory automation (TLA) has only recently been introduced in clinical microbiology in the last 10-12 years. The introduction of total laboratory automation comes with certain advantages and drawbacks that need to be assessed before the introduction of such systems in the diagnostic workflow that includes the detection of anaerobic bacteria. For several reasons, there is yet to be a manufacturer to fully address the issue of anaerobes in the setting of laboratory automation; the aim of the present paper is to address some of the issues associated with anaerobes in lab automation.
journal_name
Anaerobejournal_title
Anaerobeauthors
Gajdács Mdoi
10.1016/j.anaerobe.2019.06.009subject
Has Abstractpub_date
2019-10-01 00:00:00pages
112-114eissn
1075-9964issn
1095-8274pii
S1075-9964(19)30106-4journal_volume
59pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:A longitudinal study of Clostridium difficile colonization in piglets was performed on a conventional swine farm in Ontario, Canada. Fecal samples were collected from 10 sows prior to their expected farrowing date, and then from all their piglets on days 2, 7, 30, 44 and 62 of life. C. difficile was isolated from 4/10...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.08.001
更新日期：2010-10-01 00:00:00
abstract：:Bacteroides fragilis is a commensal bacterium found in the gut of most humans, however enterotoxigenic B. fragilis strains (ETBF) have been associated with diarrhoea and colorectal cancer (CRC). The purpose of this study was to establish a method of screening for the Bacteroides fragilis toxin (bft) gene in stool samp...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.05.004
更新日期：2016-08-01 00:00:00
abstract：:To date nine species of anaerobic intestinal spirochaetes have been validly assigned to the genus Brachyspira. These include both pathogenic and non-pathogenic species. In the current study a genomic analysis of a novel spirochaete isolate was undertaken to determine whether it is a distinct species that previously ha...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.05.004
更新日期：2019-10-01 00:00:00
abstract：:Novel polymerase chain reaction (PCR) primers designed from the 16S-23S internal transcription spacer (ITS) rRNA and 23S rRNA genes, respectively, were used for the specific detection of Lactobacillus acidophilus and Lactobacillus plantarum. Molecular weights of the PCR products were 221 and 599 bp, respectively. Stra...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.01.003
更新日期：2010-06-01 00:00:00
abstract：:This study examined whether different corn silage to grass silage ratios in ruminant rations and different grinding levels of the feed affect the composition of the ruminal Bacteroides-Prevotella community in vitro. Three diets, composed of 10% soybean meal as well as of different corn silage and grass silage proporti...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.05.002
更新日期：2010-08-01 00:00:00
abstract：:The objective of this study was to develop the strain-specific PCR primers for Fusobacterium nucleatum subsp. fusiforme ATCC 51190(T) and F. nucleatum subsp. vincentii ATCC 49256(T) based on the nucleotide sequence of the Fs17 and Fv35 DNA probes, respectively. The strain specificity was tested against 10 type strains...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.04.003
更新日期：2010-02-01 00:00:00
abstract：:Gut fungal-specific PCR primers have been used to selectively amplify the ITS1 region of gut fungal rDNA recovered from faeces of domestic and wild animals to investigate population diversity. Two different gel-based methods are described for separating populations of gut fungal rDNA amplicons, namely (1) denaturing g...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.05.003
更新日期：2010-04-01 00:00:00
abstract：:Eggerthella lenta is increasingly found in patients with severe comorbidities. Because oncologic patients are exposed to emerging pathogens, we aimed to describe the factors associated with E. lenta bacteremia in this immunosuppressed population. Oncology patients with blood cultures positive for E. lenta were retrosp...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.04.010
更新日期：2017-10-01 00:00:00
abstract：:Clostridium perfringens produces disease in sheep, goats and other animal species, most of which are generically called enterotoxemias. This micro-organism can be a normal inhabitant of the intestine of most animal species including humans, but when the intestinal environment is altered by sudden changes in diet or ot...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2003.08.005
更新日期：2004-04-01 00:00:00
abstract：:Since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. For example, reclassification of the 'Bacteroides gracilis group' reve...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.2002.0436
更新日期：2002-10-01 00:00:00
abstract：:We present the first case report of osteomyelitis due to Clostridium hydrogeniformans in a previously healthy 18-year-old male. He was admitted to our hospital because of an open contaminated fracture of the right arm after being blown into a drain in a motorbike accident. He underwent surgical debridement and treatme...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.02.013
更新日期：2017-06-01 00:00:00
abstract：:Adherence of Bilophila wadsworthia to the cultured human embryonic intestinal cell line, Intestine 407 (Int 407), varied among the strains tested from strongly adherent (76-100% cells positive for one or more adherent bacteria) to non- or weakly adherent (0-25% positive cells). Although negative staining revealed that...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0134
更新日期：1998-02-01 00:00:00
abstract：:As shown previously, a recombinant alphatoxin variant (rAT121A/91) constructed from the naturally occurring Clostridium perfringens mutant strain 121A/91, was devoid of enzymatic (PLC), hemolytic and lethal activity (18). In the present study, the recombinant variant was altered by an oligonucleotide-directed reversio...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.06.003
更新日期：2006-02-01 00:00:00
abstract：:Clostridium (Clostridioides) difficile has been identified in humans and a wide range of animal species, but there has been little study of remote animal populations with limited human contact. The objective of this study was to determine the prevalence and types of C. difficile in wild and captive polar bears (Ursus ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.03.013
更新日期：2019-06-01 00:00:00
abstract：:Members of Dysgonomonas are Gram-stain-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation from stool and wounds of human patients (CDC group DF-3). More recently, Dysgonomonas have been found to be widely distributed in terrestrial environments and are partic...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102302
更新日期：2020-12-01 00:00:00
abstract：:Recently new resin-based BacT/Alert FAN Plus bottles containing antibiotic-binding polymeric beads, were introduced as an improvement of the charcoal-based FAN bottles for the recovery of bacteria and fungi. To assess the performance of the novel anaerobic FN plus bottles in the detection of anaerobic organisms, we co...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2015.07.010
更新日期：2015-10-01 00:00:00
abstract：:The bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. Although recent studies have used DNA probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/S1075-9964(03)00109-4
更新日期：2003-10-01 00:00:00
abstract：:Sourdough application has been extensively increased in the last years due to the consumers demand for food consumption without the addition of chemical preservatives. Several starter cultures have been applied in sourdough bread making targeting the increase of bread self-life and the improvement of sensorial charact...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.03.022
更新日期：2011-12-01 00:00:00
abstract：:Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria P...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.05.013
更新日期：2018-08-01 00:00:00
abstract：:Linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant Streptococcus pneumoniae), and other aerobic and facultative bacteria. The ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/S1075-9964(03)00004-0
更新日期：2003-02-01 00:00:00
abstract：:The members of the phylum Fusobacteria and its two families, Fusobacteriaceae and Leptotrichiaceae, are distinguished at present mainly on the basis of their branching in the 16S rRNA gene trees and analysis of the internal transcribed spacer sequences in the 16S-23S rDNA. However, no biochemical or molecular characte...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.06.007
更新日期：2014-08-01 00:00:00
abstract：:Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, r...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.06.006
更新日期：2010-08-01 00:00:00
abstract：:Clostridium taeniosporum is a Gram-positive, anaerobic, rod-shaped non-toxigenic organism isolated from Crimean lake silt. It is unique in forming spores from which about twelve large, flat, ribbon-like appendages emanate. These ribbon-like structures, about 4.5 microm long and 0.45 microm wide, are assembled from sma...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.11.004
更新日期：2008-12-01 00:00:00
abstract：:Highest antimicrobial activity of peptide ST4SA (51,200 AU/mL) was recorded after 14 h of growth in MRS broth with optimal production at pH 6.0 or 6.5. Growth of strain ST4SA in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 AU/mL. An increase in production of peptide ST4SA to 10...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.11.002
更新日期：2009-06-01 00:00:00
abstract：:Clostridium difficile is a leading cause of hospital-acquired bacterial infections in the United States, and the increased incidence of recurrent C. difficile infections is particularly problematic. The molecular mechanisms of C. difficile colonization, including its ability to evade host innate immune responses, is p...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.09.004
更新日期：2012-12-01 00:00:00
abstract：:Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2015.10.013
更新日期：2016-04-01 00:00:00
abstract：:Nitrite reductase (cytochrome cd1) was purified to electrophoretic homogeneity from the soluble extract of the marine denitrifying bacterium Pseudomonas nautica strain 617. Cells were anaerobically grown with 10 mM nitrate as final electron acceptor. The soluble fraction was purified by four successive chromatographic...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1995.1021
更新日期：1995-08-01 00:00:00
abstract：:Although much newsprint is devoted to the subject of reducing the United States and other major developed countries dependence on their respective foreign energy sources; the most challenging issues for society is to provide long-term, sustainable energy sources to accommodate the global population as a whole. The pro...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.05.011
更新日期：2011-08-01 00:00:00
abstract：:Chronic periodontitis is a highly prevalent endogenous polymicrobial disease. To better understand the etiology of the disease a quantitative approach is mandatory and real-time PCR is the molecular technique currently preferred to achieve this purpose. Taking into account that such a kind of study is still scarce, we...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.02.007
更新日期：2010-06-01 00:00:00
abstract：:Susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. Orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infecti...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2004.04.003
更新日期：2004-08-01 00:00:00
