Abstract：
:Adherence of Bilophila wadsworthia to the cultured human embryonic intestinal cell line, Intestine 407 (Int 407), varied among the strains tested from strongly adherent (76-100% cells positive for one or more adherent bacteria) to non- or weakly adherent (0-25% positive cells). Although negative staining revealed that infrequent cells of an adherent strain, WAL 9077, the adherent type-strain, WAL 7959, and a non-adherent strain, WAL 8448, expressed loosely associated fimbrial structures, a role for these structures in adhesion could not be confirmed with either scanning or thin-section electron micrography. Ruthenium red staining of thin-section preparations and subsequent electron microscopy failed to reveal an extensive extracellular polysaccharide layer. SDS-PAGE analysis of crude outer membrane fractions of WAL 9077 and WAL 8448 demonstrated clear differences in their major and minor outer membrane protein components. Thus, we postulate that the adherence of B. wadsworthia to Int 407 cells is mediated by an outer membrane or cell wall component.
journal_name
Anaerobejournal_title
Anaerobeauthors
Gerardo SH,Garcia MM,Wexler HM,Finegold SMdoi
10.1006/anae.1997.0134subject
Has Abstractpub_date
1998-02-01 00:00:00pages
19-27issue
1eissn
1075-9964issn
1095-8274pii
S1075-9964(97)90134-7journal_volume
4pub_type
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