Abstract:
:Chronic periodontitis is a highly prevalent endogenous polymicrobial disease. To better understand the etiology of the disease a quantitative approach is mandatory and real-time PCR is the molecular technique currently preferred to achieve this purpose. Taking into account that such a kind of study is still scarce, we aimed to evaluate the association between periodontal microbiota and chronic periodontitis. A total of 60 low-income age-matched female adults, 30 with chronic periodontitis and 30 without periodontal disease, were enrolled. DNA obtained from subgingival specimens was used for quantification of Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia by real-time PCR. A. actinomycetemcomitans, E. corrodens, and F. nucleatum were detected in all subjects, P. gingivalis was observed in 70.0% and 46.6% and P. intermedia in 90.0% and 80.0% of chronic periodontitis patients and periodontally healthy subjects, respectively. P. gingivalis mean count was significantly higher in patients with chronic periodontitis than in periodontally healthy individuals. Accurate detection and quantification of five putative periodontal pathogens was feasible using a simple and fast real-time PCR protocol. Although P. gingivalis and P. intermedia have been found more commonly in chronic periodontitis patients, no statistical difference was observed between periodontally diseased and healthy groups. Quantitative data indicated association between P. gingivalis and chronic periodontitis. However, because of its uneven distribution, it should not be solely taken as a marker of periodontal status.
journal_name
Anaerobejournal_title
Anaerobeauthors
Braga RR,Carvalho MA,Bruña-Romero O,Teixeira RE,Costa JE,Mendes EN,Farias LM,Magalhães PPdoi
10.1016/j.anaerobe.2010.02.007subject
Has Abstractpub_date
2010-06-01 00:00:00pages
234-9issue
3eissn
1075-9964issn
1095-8274pii
S1075-9964(10)00026-0journal_volume
16pub_type
杂志文章相关文献
ANAEROBE文献大全abstract::Quorum sensing (QS) signaling regulates the motility, adhesion, and biofilm formation of bacteria, and at the same time activates immune response in eukaryotic organisms. We recently demonstrated that the QS molecule, dihydroxy-2, 3-pentanedione (DPD), and its analogs significantly inhibit estradiol-regulated virulenc...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.09.001
更新日期:2018-12-01 00:00:00
abstract::Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria P...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.05.013
更新日期:2018-08-01 00:00:00
abstract::Clostridium difficile is a leading cause of hospital-acquired bacterial infections in the United States, and the increased incidence of recurrent C. difficile infections is particularly problematic. The molecular mechanisms of C. difficile colonization, including its ability to evade host innate immune responses, is p...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2012.09.004
更新日期:2012-12-01 00:00:00
abstract::Our goal was to establish a quantitative real-time PCR (QRT-PCR) method to detect Bacteroides fragilis group and related organisms from clinical specimens. Compared to conventional anaerobic culture, QRT-PCR can provide accurate and more rapid detection and identification of B. fragilis group and similar species. B. f...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.03.004
更新日期:2011-04-01 00:00:00
abstract::Prevotella species are members of the bacterial oral flora and are opportunistic pathogens in polymicrobial infections of soft tissues. Antibiotic resistance to tetracyclines is common in these bacteria, and the gene encoding this resistance has been previously identified as tetQ. The tetQ gene is also found on conjug...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.08.008
更新日期:2010-12-01 00:00:00
abstract::The changing epidemiology of Clostridium difficile infection over the past decades presents a significant challenge in the management of C. difficile associated diseases. The gastrointestinal tract microbiota provides colonization resistance against C. difficile, and growing evidence suggests that gut microbial derive...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.03.004
更新日期:2017-06-01 00:00:00
abstract::Sixty-six nonduplicate Bacteroides clinical isolates collected at Marmara University Hospital were tested to investigate carbapenem and metronidazole resistance profiles and to detect the resistance genes (cfiA and nim) and related insertion sequence (IS) elements. The study found that there were no strains resistant ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.10.004
更新日期:2012-02-01 00:00:00
abstract::The present study aimed to elucidate the prevalence of Clostridioides difficile in Japanese retail food products. For this purpose, retail food samples (242 fresh vegetables and 266 retail meat samples: 89 chicken meat; 28 chicken liver; 200 pork meat; 24 pig liver; 127 beef meat) were collected from 14 supermarkets b...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102132
更新日期:2020-02-01 00:00:00
abstract::Injection drug use (IDU), specifically non-intravenous "skin-popping" of heroin, seems to provide optimal conditions for Clostridial infection and toxin production. IDU is therefore a major risk factor for wound botulism and Clostridial necrotizing soft tissue infections (NSTI) and continues to be linked to cases of t...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2014.09.005
更新日期:2014-12-01 00:00:00
abstract::A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.03.007
更新日期:2010-08-01 00:00:00
abstract::We report the first case of pelvic inflammatory disease (PID) caused by Actinobaculum massiliense. A 53-year-old woman attended the emergency department with symptoms compatible with a PID episode, finally resolved by intramuscular antibiotic treatment. Actinobaculum sp. was isolated by culture, and A. massiliense was...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.11.006
更新日期:2019-02-01 00:00:00
abstract::Lack of nitrogenous substrate and buffering capacity have been identified as causing failure in previous work on the treatment of fruit cordial wastewater using anaerobic continuous stirred tank reactors. In this study, ammonium bicarbonate was proposed to be used as the substrate for nitrogenous and buffering resourc...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2009.04.008
更新日期:2010-02-01 00:00:00
abstract::With the emergence of antibiotic resistance among Bacteroides fragilis group isolates the need of susceptibility testing in routine laboratories is increasing. The aims of the present study were to evaluate the disk diffusion method for susceptibility testing in case of different clinical isolates of Bacteroides spp b...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.10.008
更新日期:2015-02-01 00:00:00
abstract::Banana is a widely consumed fruit, which contains considerable amounts of potential prebiotic indigestible carbohydrates. In our randomised, controlled trial we aimed to evaluate the in vivo prebiotic effect of banana consumption on faecal microbiota. Thirty-four healthy women participated in the study, having Body Ma...
journal_title:Anaerobe
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.anaerobe.2011.03.018
更新日期:2011-12-01 00:00:00
abstract::The effect a common fecal organism, Bacteroides fragilis, has on the sporulation of Clostridium perfringens, an organism linked to some cases of antibiotic associated diarrhea, was examined. Established B. fragilis cultures significantly decreased the number of heat resistant spores formed by C. perfringens ATCC 12915...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.05.006
更新日期:2004-10-01 00:00:00
abstract::The minimum inhibitory concentration (MIC) of eight antimicrobial agents was determined by the agar dilution method for 80 isolates of Clostridium difficile from neonatal pigs with enteritis. MICs(50) for erythromycin, tilmicosin, and tylosin were relatively low (0.25-0.50 microg/mL), but MICs(90) (64 or > or =256 mic...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.01.003
更新日期:2004-02-01 00:00:00
abstract::Several studies indicated that bifidobacteria possessed strong antioxidant activity. In present study, the antioxidant activities of Bifidobacterium animalis 01 proteins were evaluated using six assays, namely, linoleic acid preoxidation assay, erythrocyte hemolysis assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, r...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.06.006
更新日期:2010-08-01 00:00:00
abstract::Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demo...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2015.10.013
更新日期:2016-04-01 00:00:00
abstract::Eight spindle-shaped bacteria were isolated from clinical samples in Japan and investigated for their taxonomic position. Phylogenetic trees (based on 16S rRNA, rpoB, zinc protease, and gyrB gene sequence comparisons) showed distinct clustering of eight strains with the type strain of Fusobacterium nucleatum and its c...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2021.102323
更新日期:2021-01-27 00:00:00
abstract::Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as oth...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.01.014
更新日期:2018-04-01 00:00:00
abstract::Firmicutes and Bacteroidetes are closely related to body fat in humans and mice, which are the two dominant bacterial divisions of gut microbiota in mammals. Here real-time PCR analysis indicated that Meishan pigs had a 34% reduction in percentage Bacteroidetes (P = 0.008) and a significantly lower proportion of Bacte...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2008.04.001
更新日期:2008-10-01 00:00:00
abstract::A longitudinal study of Clostridium difficile colonization in piglets was performed on a conventional swine farm in Ontario, Canada. Fecal samples were collected from 10 sows prior to their expected farrowing date, and then from all their piglets on days 2, 7, 30, 44 and 62 of life. C. difficile was isolated from 4/10...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.08.001
更新日期:2010-10-01 00:00:00
abstract::The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012-2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.01.010
更新日期:2019-04-01 00:00:00
abstract::Clostridium septicum is responsible for a number of diseases, the most serious of which are a frequently fatal non-traumatic gas gangrene in man and braxy malignant oedema and blackquarter in farm animals. Immunity to these diseases is mediated mainly by antitoxin raised against C. septicum alpha toxin, which has hemo...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1997.0105
更新日期:1997-04-01 00:00:00
abstract::The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2017.01.001
更新日期:2017-04-01 00:00:00
abstract::Here, we report a case of fatal sepsis resulting from an intra-abdominal infection caused by a Bacteroides fragilis strain containing a CfiA4 metallo-β-lactamase and an upstream insertion sequence (IS) element. Meropenem was used as empiric therapy for septic shock as a result of the intra-abdominal infection, althoug...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.01.010
更新日期:2017-04-01 00:00:00
abstract::The toxic effects of heavy metals have been extensively documented in different organisms. Nevertheless, a lack of information exists with regard to this topic in the case of autochthonous microorganism communities. The aim of this study was to evaluate the toxic effects of zinc on the anaerobic microorganisms present...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2007.01.001
更新日期:2007-04-01 00:00:00
abstract::Treponema denticola is a spirochete that is involved in causing periodontal diseases. This bacterium can produce H2S from thiol compounds found in the gingival crevicular fluid. Determining how H2S is made by oral bacteria is important since this molecule is present at high levels in periodontally-diseased pockets and...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2020.102231
更新日期:2020-08-01 00:00:00
abstract::Bacteroides fragilis is a commensal bacterium found in the gut of most humans, however enterotoxigenic B. fragilis strains (ETBF) have been associated with diarrhoea and colorectal cancer (CRC). The purpose of this study was to establish a method of screening for the Bacteroides fragilis toxin (bft) gene in stool samp...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2016.05.004
更新日期:2016-08-01 00:00:00
abstract::Enterotoxigenic Bacteroides fragilis (ETBF) are considered as an emerging enteropathogen causing diarrhea in children. Eight hundred and thirty-six (836) children less than 5 years of age including 587 children with diarrhea and 249 age-matched controls were involved in the study. Within the group of children with dia...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.10.004
更新日期:2005-02-01 00:00:00