Abstract:
:A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.
journal_name
Anaerobejournal_title
Anaerobeauthors
Guo DH,Sun DB,Wu R,Yang HM,Zheng JS,Fan CL,Sun B,Wang JFdoi
10.1016/j.anaerobe.2010.03.007subject
Has Abstractpub_date
2010-08-01 00:00:00pages
317-20issue
4eissn
1075-9964issn
1095-8274pii
S1075-9964(10)00033-8journal_volume
16pub_type
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