An indirect ELISA for serodiagnosis of cattle footrot caused by Fusobacterium necrophorum.

Abstract:

:A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Guo DH,Sun DB,Wu R,Yang HM,Zheng JS,Fan CL,Sun B,Wang JF

doi

10.1016/j.anaerobe.2010.03.007

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

317-20

issue

4

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(10)00033-8

journal_volume

16

pub_type

杂志文章

相关文献

ANAEROBE文献大全
  • Phylogeny and molecular signatures for the phylum Fusobacteria and its distinct subclades.

    abstract::The members of the phylum Fusobacteria and its two families, Fusobacteriaceae and Leptotrichiaceae, are distinguished at present mainly on the basis of their branching in the 16S rRNA gene trees and analysis of the internal transcribed spacer sequences in the 16S-23S rDNA. However, no biochemical or molecular characte...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2014.06.007

    authors: Gupta RS,Sethi M

    更新日期:2014-08-01 00:00:00

  • Antimicrobial susceptibility of Clostridium difficile isolated from neonatal pigs with enteritis.

    abstract::The minimum inhibitory concentration (MIC) of eight antimicrobial agents was determined by the agar dilution method for 80 isolates of Clostridium difficile from neonatal pigs with enteritis. MICs(50) for erythromycin, tilmicosin, and tylosin were relatively low (0.25-0.50 microg/mL), but MICs(90) (64 or > or =256 mic...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2004.01.003

    authors: Post KW,Songer JG

    更新日期:2004-02-01 00:00:00

  • Capnocytophaga spp. infection causing chorioamnionitis: an unusual suspect.

    abstract::Capnocytophaga spp. are not often considered as a cause of perinatal infection. However, Capnocytophaga spp. are a commensal of the human oral cavity and can be opportunistic pathogens. The present case illustrates the ability of a species from the human oral cavity, C. sputigena, to cause an ascending infection of th...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2018.07.006

    authors: Felix L,Rosenberg A,Caraballo KA,Taborga DP,Hamula C

    更新日期:2019-10-01 00:00:00

  • Prevalence of CTGCAG recognizing restriction and modification systems in ruminal selenomonades.

    abstract::Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1006/anae.1998.0180

    authors: Molnarova V,Pristas P,Javorsky P

    更新日期:1999-02-01 00:00:00

  • Inhibition of spore germination, growth, and toxin activity of clinically relevant C. difficile strains by gut microbiota derived secondary bile acids.

    abstract::The changing epidemiology of Clostridium difficile infection over the past decades presents a significant challenge in the management of C. difficile associated diseases. The gastrointestinal tract microbiota provides colonization resistance against C. difficile, and growing evidence suggests that gut microbial derive...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.03.004

    authors: Thanissery R,Winston JA,Theriot CM

    更新日期:2017-06-01 00:00:00

  • An in vitro time-kill assessment of linezolid and anaerobic bacteria.

    abstract::Linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant Streptococcus pneumoniae), and other aerobic and facultative bacteria. The ...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/S1075-9964(03)00004-0

    authors: Yagi BH,Zurenko GE

    更新日期:2003-02-01 00:00:00

  • Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity.

    abstract::Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as oth...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2018.01.014

    authors: Castro J,Martins AP,Rodrigues ME,Cerca N

    更新日期:2018-04-01 00:00:00

  • In vitro comparative evaluation of the impact of lacto-N-biose I, a major building block of human milk oligosaccharides, on the fecal microbiota of infants.

    abstract::Lacto-N-biose I (LNB) is a potential factor for the selective growth of bifidobacteria. We previously reported that the species of bifidobacteria predominant in infant intestines might use LNB. We aimed to assess the prebiotic properties of LNB in comparison to other oligosaccharides using an in vitro fermentation sys...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.12.007

    authors: Satoh T,Odamaki T,Namura M,Shimizu T,Iwatsuki K,Nishimoto M,Kitaoka M,Xiao JZ

    更新日期:2013-02-01 00:00:00

  • Fatal sepsis caused by multidrug-resistant Bacteroides fragilis, harboring a cfiA gene and an upstream insertion sequence element, in Japan.

    abstract::Here, we report a case of fatal sepsis resulting from an intra-abdominal infection caused by a Bacteroides fragilis strain containing a CfiA4 metallo-β-lactamase and an upstream insertion sequence (IS) element. Meropenem was used as empiric therapy for septic shock as a result of the intra-abdominal infection, althoug...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.01.010

    authors: Nakamura I,Aoki K,Miura Y,Yamaguchi T,Matsumoto T

    更新日期:2017-04-01 00:00:00

  • Lactoferrin and lactoferricin B reduce adhesion and biofilm formation in the intestinal symbionts Bacteroides fragilis and Bacteroides thetaiotaomicron.

    abstract::Several factors affect the composition of species that inhabit our intestinal tract, including mode of delivery, genetics and nutrition. Antimicrobial peptides and proteins secreted in the gastrointestinal tract are powerful tools against bacteria. Lactoferrin (LF) inhibits the growth of several bacterial species, suc...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2020.102232

    authors: de Sá Almeida JS,de Oliveira Marre AT,Teixeira FL,Boente RF,Domingues RMCP,de Paula GR,Lobo LA

    更新日期:2020-08-01 00:00:00

  • A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.

    abstract::This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.05.001

    authors: Ben Salah R,Trabelsi I,Ben Mansour R,Lassoued S,Chouayekh H,Bejar S

    更新日期:2012-08-01 00:00:00

  • Determination of immunological homology between cellulosome subunits and cloned endoglucanases and xylanase of Clostridium thermocellum.

    abstract::Immuno-cross reactivity between the subunits of Clostridium thermocellum cellulosome and cloned endogucanases and xylanase from the same bacterium was studied using the polyclonal antibodies raised against cloned enzymes. Dot blot analysis showed that the cellulosome, S8 and S11 subunits cross-reacted strongly with th...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1006/anae.1997.0119

    authors: Bhat S,Hutson RA,Owen E,Bhat MK

    更新日期:1997-10-01 00:00:00

  • Use of PCR primers and probes based on the 23S rRNA and internal transcription spacer (ITS) gene sequence for the detection and enumerization of Lactobacillus acidophilus and Lactobacillus plantarum in feed supplements.

    abstract::Novel polymerase chain reaction (PCR) primers designed from the 16S-23S internal transcription spacer (ITS) rRNA and 23S rRNA genes, respectively, were used for the specific detection of Lactobacillus acidophilus and Lactobacillus plantarum. Molecular weights of the PCR products were 221 and 599 bp, respectively. Stra...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2010.01.003

    authors: Tsai CC,Lai CH,Yu B,Tsen HY

    更新日期:2010-06-01 00:00:00

  • Determination of the Clostridium perfringens-binding site on fibronectin.

    abstract::The extracellular matrix protein fibronectin (Fn) is known to bind to the surface of Clostridium perfringens cells. Fn is a disulfide-linked homodimer protein, with each Fn polypeptide consisting of three types of repeating modules: 12 type I, 2 type II, and 15-17 type III modules. To determine the epitope on Fn recog...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2014.11.007

    authors: Katayama S,Tagomori M,Morita N,Yamasaki T,Nariya H,Okada M,Watanabe M,Hitsumoto Y

    更新日期:2015-08-01 00:00:00

  • The first reported case of pelvic inflammatory disease caused by Actinobaculum massiliense.

    abstract::We report the first case of pelvic inflammatory disease (PID) caused by Actinobaculum massiliense. A 53-year-old woman attended the emergency department with symptoms compatible with a PID episode, finally resolved by intramuscular antibiotic treatment. Actinobaculum sp. was isolated by culture, and A. massiliense was...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2018.11.006

    authors: Carrillo-Ávila JA,Bonilla-García L,Navarro-Marí JM,Gutiérrez-Fernández J

    更新日期:2019-02-01 00:00:00

  • Relationship between gastrointestinal dysbiosis and Clostridium botulinum in dairy cows.

    abstract::The gastrointestinal tract is a balanced ecosystem that can get out of balance and predisposed to clostridial diseases or other pathological conditions. The objective of the present study was to evaluate the gut microbiota in dairy cows suffering from chronic botulism. Cows were investigated for Clostridium (C.) botul...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2014.03.013

    authors: Krüger M,Shehata AA,Grosse-Herrenthey A,Ständer N,Schrödl W

    更新日期:2014-06-01 00:00:00

  • Delayed infection with Parvimonas micra following spinal instrumentation.

    abstract:BACKGROUND CONTEXT:Delayed-onset surgical site infections following spinal instrumentation are uncommon and often present with chronic pain and implant failure. Anaerobic organisms are rarely implicated and identified with difficulty in these infections. PURPOSE:We report a case of vertebral osteomyelitis and epidural...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2015.08.004

    authors: George IA,Pande A,Parsaei S

    更新日期:2015-10-01 00:00:00

  • Antimicrobial susceptibility profiles of oral Treponema species.

    abstract::Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontit...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.10.005

    authors: Okamoto-Shibayama K,Sekino J,Yoshikawa K,Saito A,Ishihara K

    更新日期:2017-12-01 00:00:00

  • Establishment of valnemulin susceptibility breakpoint against Clostridium perfringens in rabbits.

    abstract::Susceptibility breakpoints provide fundamental information for rational administration of antibiotics. The present investigation reports the first valnemulin susceptibility breakpoint (MIC<0.25 μg/mL) against Clostridium perfringens infections in rabbits based on the wild-type cutoff (COWT) and the pharmacokinetics/ph...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.08.006

    authors: Tao MT,Zhou YF,Sun J,Liu YH,Liao XP

    更新日期:2017-12-01 00:00:00

  • Proteotyping of laboratory-scale biogas plants reveals multiple steady-states in community composition.

    abstract::Complex microbial communities are the functional core of anaerobic digestion processes taking place in biogas plants (BGP). So far, however, a comprehensive characterization of the microbiomes involved in methane formation is technically challenging. As an alternative, enriched communities from laboratory-scale experi...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.02.005

    authors: Kohrs F,Heyer R,Bissinger T,Kottler R,Schallert K,Püttker S,Behne A,Rapp E,Benndorf D,Reichl U

    更新日期:2017-08-01 00:00:00

  • Biodiversity and fermentative activity of caecal microbial communities in wild and farm rabbits from Spain.

    abstract::In order to study the microbial caecal ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.04.004

    authors: Abecia L,Rodríguez-Romero N,Yañez-Ruiz DR,Fondevila M

    更新日期:2012-06-01 00:00:00

  • Effect of DNA extraction and sample preservation method on rumen bacterial population.

    abstract::The comparison of the bacterial profile of intracellular (iDNA) and extracellular DNA (eDNA) isolated from cow rumen content stored under different conditions was conducted. The influence of rumen fluid treatment (cheesecloth squeezed, centrifuged, filtered), storage temperature (RT, -80 °C) and cryoprotectants (PBS-g...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2013.09.015

    authors: Fliegerova K,Tapio I,Bonin A,Mrazek J,Callegari ML,Bani P,Bayat A,Vilkki J,Kopečný J,Shingfield KJ,Boyer F,Coissac E,Taberlet P,Wallace RJ

    更新日期:2014-10-01 00:00:00

  • Screening for enterotoxigenic Bacteroides fragilis in stool samples.

    abstract::Bacteroides fragilis is a commensal bacterium found in the gut of most humans, however enterotoxigenic B. fragilis strains (ETBF) have been associated with diarrhoea and colorectal cancer (CRC). The purpose of this study was to establish a method of screening for the Bacteroides fragilis toxin (bft) gene in stool samp...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2016.05.004

    authors: Keenan JI,Aitchison A,Purcell RV,Greenlees R,Pearson JF,Frizelle FA

    更新日期:2016-08-01 00:00:00

  • Impact of the introduction of nucleic acid amplification testing on Clostridioides difficile detection and ribotype distribution in Wales.

    abstract:OBJECTIVES:To determine the impact of the 2018 introduction of nucleic acid amplification tests (NAATs) for C. difficile detection on the laboratory diagnosis of C. difficile infection (CDI), and the distribution of C. difficile ribotypes. METHODS:A retrospective analysis of five years (2015-2019) of C. difficile diag...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2020.102313

    authors: Perry MD,White PL,Morris TE

    更新日期:2020-12-09 00:00:00

  • Diagnosis of Clostridium perfringens intestinal infections in sheep and goats.

    abstract::Clostridium perfringens produces disease in sheep, goats and other animal species, most of which are generically called enterotoxemias. This micro-organism can be a normal inhabitant of the intestine of most animal species including humans, but when the intestinal environment is altered by sudden changes in diet or ot...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2003.08.005

    authors: Uzal FA

    更新日期:2004-04-01 00:00:00

  • The non-enzymatic reduction of azo dyes by flavin and nicotinamide cofactors under varying conditions.

    abstract::Azo dyes are ubiquitous in products and often become environmental pollutants due to their anthropogenic nature. Azoreductases are enzymes which are present within many bacteria and are capable of breaking down the azo dyes via reduction of the azo bond. Often, though, carcinogenic aromatic amines are formed as metabo...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2013.07.005

    authors: Morrison JM,John GH

    更新日期:2013-10-01 00:00:00

  • Probiotic properties of lactobacilli species isolated from children's feces.

    abstract::In the present research, the 20 lactobacilli isolated from children feces aged 4-15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected ant...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2013.09.006

    authors: Tulumoglu S,Yuksekdag ZN,Beyatli Y,Simsek O,Cinar B,Yaşar E

    更新日期:2013-12-01 00:00:00

  • Occurrence and activity of human intestinal bacteria involved in the conversion of dietary lignans.

    abstract::The human intestinal microbiota is necessary for the production of enterolignans from the dietary lignan secoisolariciresinol diglucoside (SDG). However, little is known about the bacteria that contribute to SDG conversion. Therefore, we aimed at describing the occurrence and activity of SDG metabolising bacteria. The...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2005.11.002

    authors: Clavel T,Borrmann D,Braune A,Doré J,Blaut M

    更新日期:2006-06-01 00:00:00

  • Molecular characterization of the diversity of Clostridium chauvoei isolates collected from two bovine slaughterhouses: analysis of cross-contamination.

    abstract::Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rate disease affecting mainly cattle and sheep. Carcasses of animals affected by the disease are the chief source of soil infection and considered as an ever-present threat to livestock health. A study was undertaken to examine the cross-contami...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2008.01.004

    authors: Sathish S,Swaminathan K

    更新日期:2008-06-01 00:00:00

  • Clostridium taeniosporum is a close relative of the Clostridium botulinum Group II.

    abstract::Clostridium taeniosporum is a Gram-positive, anaerobic, rod-shaped non-toxigenic organism isolated from Crimean lake silt. It is unique in forming spores from which about twelve large, flat, ribbon-like appendages emanate. These ribbon-like structures, about 4.5 microm long and 0.45 microm wide, are assembled from sma...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2008.11.004

    authors: Iyer AV,Blinkova AL,Yang SY,Harrison M,Tepp WH,Jacobson MJ,Johnson EA,Bennett GN,Walker JR

    更新日期:2008-12-01 00:00:00