Abstract:
:In the present research, the 20 lactobacilli isolated from children feces aged 4-15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected antibiotics. All the lactobacilli strains were able to survive in low pH and bile salt conditions at pH 2.0 and 0.25% bile salt for 2 h. Moreover, all lactobacilli strains exhibited inhibitory activity against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213. In addition, all lactobacilli strains indicated resistance to teicoplanin, vancomycin, and bacitracin. The amount of exopolysaccharide (EPS) produced by the strains was 70 and 290 mg/L. The capabilities to autoaggregation and coaggregate with E. coli ATCC 11229 of the strains were also evaluated. High EPS-producing strains indicated significant autoaggregation and coaggregation capability with test bacteria (p < 0.01). The maximum cholesterol removal (76.5%) was observed by strain Lactobacillus pentosus T3, producing a high amount of exopolysaccharide, in 0.3%oxgall concentration (p < 0.05). Our results demonstrate that the capability to EPS production, acid-bile tolerance, antimicrobial activity, antibiotic resistance, aggregation and cholesterol removal of lactobacilli could be utilized for preliminary screening in order to identify potentially probiotic bacteria suitable for human.
journal_name
Anaerobejournal_title
Anaerobeauthors
Tulumoglu S,Yuksekdag ZN,Beyatli Y,Simsek O,Cinar B,Yaşar Edoi
10.1016/j.anaerobe.2013.09.006subject
Has Abstractpub_date
2013-12-01 00:00:00pages
36-42eissn
1075-9964issn
1095-8274pii
S1075-9964(13)00154-6journal_volume
24pub_type
杂志文章相关文献
ANAEROBE文献大全abstract:BACKGROUND:The incidence of Clostridium difficile infections (CDI) in the Clinical Center of Serbia (CCS) and the entire Serbia has been constantly rising in the previous 5 years. We aimed to study C. difficile PCR-ribotypes isolated from patients hospitalized at two healthcare institutions: CCS and Specialized Hospita...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.04.005
更新日期:2018-06-01 00:00:00
abstract::Lifestyle and dietary habits are crucial features that can alter the gut microbiome of humans. Humans, along with their gut microbes, have coevolved in order to sustain themselves in different environments. They were able to adapt themselves to the dietary sources available in their environment. The relation between h...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2016.03.002
更新日期:2016-06-01 00:00:00
abstract::The gastrointestinal tract is a balanced ecosystem that can get out of balance and predisposed to clostridial diseases or other pathological conditions. The objective of the present study was to evaluate the gut microbiota in dairy cows suffering from chronic botulism. Cows were investigated for Clostridium (C.) botul...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.03.013
更新日期:2014-06-01 00:00:00
abstract::Recent genomic analysis of an epidemic ribotype 027 (RT027) Clostridioides difficile strain revealed the presence of several chromosomal site-specific invertible sites hypothesized to control the expression of adjacent genes in a bimodal on-off mode. This process, named phase variation, is thought to enhance phenotypi...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102073
更新日期:2019-12-01 00:00:00
abstract::The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibi...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.04.007
更新日期:2017-10-01 00:00:00
abstract::Quorum sensing (QS) signaling regulates the motility, adhesion, and biofilm formation of bacteria, and at the same time activates immune response in eukaryotic organisms. We recently demonstrated that the QS molecule, dihydroxy-2, 3-pentanedione (DPD), and its analogs significantly inhibit estradiol-regulated virulenc...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.09.001
更新日期:2018-12-01 00:00:00
abstract::Community-associated Clostridium difficile infection (CA-CDI) represents 32% of all CDI cases based on U.S. population-based data. The current epidemic strain, NAP1, is the most prevalent strain causing these infections. Although complications, recurrence and death are uncommon, one-fourth of the CA-CDI patients are h...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.01.006
更新日期:2013-12-01 00:00:00
abstract::Since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. For example, reclassification of the 'Bacteroides gracilis group' reve...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.2002.0436
更新日期:2002-10-01 00:00:00
abstract::The aim of this study was to investigate Clostridium difficile and Clostridium perfringens in 82 diarrheic dogs positive for canine parvovirus type 2 (CPV). Enterotoxigenic C. perfringens type A was isolated from three (3.6%) dogs. One (1.2%) strain was also positive for NetE- and NetF-encoding genes, which are common...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.07.001
更新日期:2017-12-01 00:00:00
abstract::Linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant Streptococcus pneumoniae), and other aerobic and facultative bacteria. The ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/S1075-9964(03)00004-0
更新日期:2003-02-01 00:00:00
abstract::Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1998.0180
更新日期:1999-02-01 00:00:00
abstract::Bifidobacteria are part of the human gastrointestinal microbiota and are used as probiotics in functional food products because of their health promoting properties. However, only few data are available on the phenotypic characteristics displayed by human bifidobacteria strain populations. In this study we compared th...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.04.005
更新日期:2013-06-01 00:00:00
abstract::Clostridium (Clostridioides) difficile is a major cause of nosocomial diarrhoea. A first inter-laboratory ring trial was performed in four European countries to evaluate the genotyping and antibiotic susceptibility testing (AST) accuracy. Six C. difficile isolates representing the epidemiologic important ribotypes (RT...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102093
更新日期:2020-02-01 00:00:00
abstract::Susceptibility breakpoints provide fundamental information for rational administration of antibiotics. The present investigation reports the first valnemulin susceptibility breakpoint (MIC<0.25 μg/mL) against Clostridium perfringens infections in rabbits based on the wild-type cutoff (COWT) and the pharmacokinetics/ph...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.08.006
更新日期:2017-12-01 00:00:00
abstract::Clostridium (Clostridioides) difficile has been identified in humans and a wide range of animal species, but there has been little study of remote animal populations with limited human contact. The objective of this study was to determine the prevalence and types of C. difficile in wild and captive polar bears (Ursus ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.03.013
更新日期:2019-06-01 00:00:00
abstract::In this paper, the bioactivity of cell-free extracts obtained by dairy propionibacteria strains was investigated. Probiotic bifidobacteria and lactobacilli were used as microbial targets. The extracellular filtrates were added as ingredient (1% v/v) into the growth medium; the effect of cell-free filtrates was evaluat...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.09.016
更新日期:2014-12-01 00:00:00
abstract::This study examined whether different corn silage to grass silage ratios in ruminant rations and different grinding levels of the feed affect the composition of the ruminal Bacteroides-Prevotella community in vitro. Three diets, composed of 10% soybean meal as well as of different corn silage and grass silage proporti...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.05.002
更新日期:2010-08-01 00:00:00
abstract::Novel polymerase chain reaction (PCR) primers designed from the 16S-23S internal transcription spacer (ITS) rRNA and 23S rRNA genes, respectively, were used for the specific detection of Lactobacillus acidophilus and Lactobacillus plantarum. Molecular weights of the PCR products were 221 and 599 bp, respectively. Stra...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.01.003
更新日期:2010-06-01 00:00:00
abstract::Here, we report a case of fatal sepsis resulting from an intra-abdominal infection caused by a Bacteroides fragilis strain containing a CfiA4 metallo-β-lactamase and an upstream insertion sequence (IS) element. Meropenem was used as empiric therapy for septic shock as a result of the intra-abdominal infection, althoug...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.01.010
更新日期:2017-04-01 00:00:00
abstract::Three cases of Clostridium aldenense bacteremia are reported. C. aldenense is also associated with intra-abdominal infections and closely resembles Clostridium clostridioforme and therefore may be misidentified. C. aldenense may be a more frequent pathogen than appreciated and is generally fluoroquinolone resistant. ...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2010.08.004
更新日期:2010-10-01 00:00:00
abstract::Sporulation during Clostridioides difficile infection (CDI) contributes to recurrent disease. Cell division and sporulation both require peptidoglycan biosynthesis. We show C. difficile growth and sporulation is attenuated by antisenses to murA and murC or the MurA inhibitor fosfomycin. Thus, targeting the early steps...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102129
更新日期:2020-02-01 00:00:00
abstract::Cellulase family and some other glycosyl hydrolases of anaerobic fungi inhabiting the digestive tract of ruminants are believed to form an enzyme complex called cellulosome. Study of the individual component of cellulosome may shed light on understanding the organization of this complex and its functional mechanism. W...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/S1075-9964(03)00087-8
更新日期:2003-06-01 00:00:00
abstract::Bacteroides fragilis is a commensal bacterium found in the gut of most humans, however enterotoxigenic B. fragilis strains (ETBF) have been associated with diarrhoea and colorectal cancer (CRC). The purpose of this study was to establish a method of screening for the Bacteroides fragilis toxin (bft) gene in stool samp...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2016.05.004
更新日期:2016-08-01 00:00:00
abstract::Clostridioides difficile is the leading cause of healthcare-associated diarrhea. Although the incidence of C. difficile-associated diarrhea is increasing worldwide, bacteremia due to C. difficile is relatively rare and represents the least frequent extra-colonic manifestation of C. difficile infection. To date, only 6...
journal_title:Anaerobe
pub_type:
doi:10.1016/j.anaerobe.2020.102195
更新日期:2020-06-01 00:00:00
abstract::Diagnostic laboratories are urged to take advantage of novel technological advancements to provide standardized and high-throughput information for clinicians; however, total laboratory automation (TLA) has only recently been introduced in clinical microbiology in the last 10-12 years. The introduction of total labora...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.06.009
更新日期:2019-10-01 00:00:00
abstract::Some Australian strain types of Clostridium difficile appear unique, highlighting the global diversity of this bacterium. We examined recent and historic local isolates, finding predominantly toxinotype 0 strains, but also toxinotypes V and VIII. All isolates tested were susceptible to vancomycin and metronidazole, wh...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2015.05.001
更新日期:2015-08-01 00:00:00
abstract::The aim of this study was to determine the microbial profile of periodontal lesions in HIV seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. The subgingival microflora of 20 CDC II, 20 CDC III, 20 CDC IV/V and 20 systemically healthy patients with ra...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1997.0081
更新日期:1997-04-01 00:00:00
abstract::Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demo...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2015.10.013
更新日期:2016-04-01 00:00:00
abstract::Sourdough application has been extensively increased in the last years due to the consumers demand for food consumption without the addition of chemical preservatives. Several starter cultures have been applied in sourdough bread making targeting the increase of bread self-life and the improvement of sensorial charact...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.03.022
更新日期:2011-12-01 00:00:00
abstract::Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontit...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.10.005
更新日期:2017-12-01 00:00:00