Abstract：
:Dental plaque is a multispecies biofilm in the oral cavity that significantly influences oral health. The presence of the oral anaerobic pathogen Porphyromonas gingivalis is an important determinant in the development of periodontitis. Direct and indirect interactions between P. gingivalis and the host play a major role in disease development. Transcriptome analysis recently revealed that P. gingivalis gene-expression is regulated by LuxS in both an AI-2-dependent and an AI-2 independent manner. However, little is known about the role of LuxS-signaling in P. gingivalis-host interactions. Here, we investigated the effect of a luxS mutation on the ability of P. gingivalis to induce an inflammatory response in human oral cells in vitro. Primary periodontal ligament (PDL) fibroblasts were challenged with P. gingivalis ΔluxS or the wild-type parental strain and gene-expression of pro-inflammatory mediators IL-1β, IL-6 and MCP-1 was determined by real-time PCR. The ability of P. gingivalis ΔluxS to induce an inflammatory response was severely impaired in PDL-fibroblasts. This phenotype could be restored by providing of LuxS in trans, but not by addition of the AI-2 precursor DPD. A similar phenomenon was observed in a previous transcriptome study showing that expression of PGN_0482 was reduced in the luxS mutant independently of AI-2. We therefore also analyzed the effect of a mutation in PGN_0482, which encodes an immuno-reactive, putative outer-membrane protein. Similar to P. gingivalis ΔluxS, the P. gingivalis Δ0482 mutant had an impaired ability to induce an inflammatory response in PDL fibroblasts. LuxS thus appears to influence the pro-inflammatory responses of host cells to P. gingivalis, likely through regulation of PGN_0482.
journal_name
Anaerobejournal_title
Anaerobeauthors
Scheres N,Lamont RJ,Crielaard W,Krom BPdoi
10.1016/j.anaerobe.2014.11.011subject
Has Abstractpub_date
2015-10-01 00:00:00pages
3-9issue
Pt Aeissn
1075-9964issn
1095-8274pii
S1075-9964(14)00171-1journal_volume
35pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:We studied the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against common pathogens associated with complicated intra-abdominal infections. Ten healthy volunteers received a single dose of moxifloxacin (400 mg) and levofloxacin (750 mg) and serum samples were collected at 2, 4, 8, 12, and 24h af...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.09.005
更新日期：2008-02-01 00:00:00
abstract：:Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) was estimated by 16S rDNA homology analysis. Two rumen 16S rDNA libraries were constructed. Of the 194 clones in the library of yak rumen, the sequences were mainly clustered to two phyla, low G+C Gram-positive bacteria (LGCGPB, 54...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.02.001
更新日期：2005-08-01 00:00:00
abstract：:The members of the phylum Fusobacteria and its two families, Fusobacteriaceae and Leptotrichiaceae, are distinguished at present mainly on the basis of their branching in the 16S rRNA gene trees and analysis of the internal transcribed spacer sequences in the 16S-23S rDNA. However, no biochemical or molecular characte...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.06.007
更新日期：2014-08-01 00:00:00
abstract：:Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-char...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.08.014
更新日期：2017-12-01 00:00:00
abstract：:To investigate whether there is a nexus between the microbial diversity level (taxonomic, functional and ecological) and the stress tolerance potential of the microbial community, the impact of different ammonium sources was evaluated. Therefore reactors adapted either to the anaerobic digestions of sugar beet silage ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.01.003
更新日期：2019-04-01 00:00:00
abstract：:Immuno-cross reactivity between the subunits of Clostridium thermocellum cellulosome and cloned endogucanases and xylanase from the same bacterium was studied using the polyclonal antibodies raised against cloned enzymes. Dot blot analysis showed that the cellulosome, S8 and S11 subunits cross-reacted strongly with th...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0119
更新日期：1997-10-01 00:00:00
abstract：:The purpose of this article is to set out some important considerations on the main emerging antibiotic resistance patterns among anaerobic bacteria. The first point concerns the Bacteroides fragilis group and its resistance to the combination of β-lactam+β-lactamase inhibitor. When there is overproduction of cephalos...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2010.10.002
更新日期：2010-12-01 00:00:00
abstract：:Clostridium (Clostridioides) difficile has been identified in humans and a wide range of animal species, but there has been little study of remote animal populations with limited human contact. The objective of this study was to determine the prevalence and types of C. difficile in wild and captive polar bears (Ursus ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.03.013
更新日期：2019-06-01 00:00:00
abstract：:Prevotella dentalis is a Gram-negative anaerobic rod involved in various human diseases, especially oral infections. We report a rare case of a pleural effusion due to this microorganism in an elderly patient. An 88-year-old man with chronic respiratory disease presented with a left pleural effusion for more than 1 mo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.09.004
更新日期：2018-12-01 00:00:00
abstract：:Clostridium perfringens isolates associated with food poisoning carries a chromosomal cpe gene, while non-foodborne human gastrointestinal disease isolates carry a plasmid cpe gene. The enterotoxigenic strains tested produced vegetative cells and spores with significantly higher resistance than non-enterotoxigenic str...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.03.007
更新日期：2016-06-01 00:00:00
abstract：:The aim of the study was to purify the exopolysaccharides (EPS) produced from Bifidobacterium animalis RH, which was isolated from the feces of Bama centenarians in Guangxi of China, and evaluate their antioxidant activities in vitro and in vivo. 2 fractions, a neutral EPS fraction (EPSa) and an acidic EPS fraction (E...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.07.010
更新日期：2011-10-01 00:00:00
abstract：:The human intestinal microbiota is necessary for the production of enterolignans from the dietary lignan secoisolariciresinol diglucoside (SDG). However, little is known about the bacteria that contribute to SDG conversion. Therefore, we aimed at describing the occurrence and activity of SDG metabolising bacteria. The...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.11.002
更新日期：2006-06-01 00:00:00
abstract：:The objective of this work was to compare the biomass and community structure of bacteria, protozoa and archaea communities in samples of rumen and faeces of goats and to what extent the diet (alfalfa hay with or without supplemented oats) offered to them exert an influence. Four cannulated adult goats fistulated in t...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.11.012
更新日期：2014-10-01 00:00:00
abstract：:With the emergence of antibiotic resistance among Bacteroides fragilis group isolates the need of susceptibility testing in routine laboratories is increasing. The aims of the present study were to evaluate the disk diffusion method for susceptibility testing in case of different clinical isolates of Bacteroides spp b...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.10.008
更新日期：2015-02-01 00:00:00
abstract：:Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rate disease affecting mainly cattle and sheep. Carcasses of animals affected by the disease are the chief source of soil infection and considered as an ever-present threat to livestock health. A study was undertaken to examine the cross-contami...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.01.004
更新日期：2008-06-01 00:00:00
abstract：:Several studies have suggested the association of disturbed genital tract microbiota with infertility. Our aim was to clarify the influence of sexual intercourse on partner's genital tract microbiota in infertile couples. Seventeen couples were studied, and in 5 men inflammatory prostatitis (IP) was diagnosed. Semen s...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.04.015
更新日期：2011-12-01 00:00:00
abstract：:Sixty-six nonduplicate Bacteroides clinical isolates collected at Marmara University Hospital were tested to investigate carbapenem and metronidazole resistance profiles and to detect the resistance genes (cfiA and nim) and related insertion sequence (IS) elements. The study found that there were no strains resistant ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.10.004
更新日期：2012-02-01 00:00:00
abstract：:Some Australian strain types of Clostridium difficile appear unique, highlighting the global diversity of this bacterium. We examined recent and historic local isolates, finding predominantly toxinotype 0 strains, but also toxinotypes V and VIII. All isolates tested were susceptible to vancomycin and metronidazole, wh...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2015.05.001
更新日期：2015-08-01 00:00:00
abstract：:The toxic effects of heavy metals have been extensively documented in different organisms. Nevertheless, a lack of information exists with regard to this topic in the case of autochthonous microorganism communities. The aim of this study was to evaluate the toxic effects of zinc on the anaerobic microorganisms present...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.01.001
更新日期：2007-04-01 00:00:00
abstract：:Recurrent Clostridioides (formerly Clostridium) difficile infection (rCDI) is common, and patients who have had one recurrence are more likely to have multiple recurrences. Frequent recurrences have been associated with increased morbidity and mortality, high healthcare costs, and lower quality of life. In this review...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.102098
更新日期：2020-02-01 00:00:00
abstract：:The gastrointestinal tract is a balanced ecosystem that can get out of balance and predisposed to clostridial diseases or other pathological conditions. The objective of the present study was to evaluate the gut microbiota in dairy cows suffering from chronic botulism. Cows were investigated for Clostridium (C.) botul...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.03.013
更新日期：2014-06-01 00:00:00
abstract：:Mastoiditis (M) is the most common intratemporal complication of otitis media. The incidence of M has decreased since the advent of antimicrobial agents. In the last decade, however, there has been a marked increased in the incidence of acute M in several communities, sometimes in association with the growing resistan...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.03.005
更新日期：2005-10-01 00:00:00
abstract：:Complex microbial communities are the functional core of anaerobic digestion processes taking place in biogas plants (BGP). So far, however, a comprehensive characterization of the microbiomes involved in methane formation is technically challenging. As an alternative, enriched communities from laboratory-scale experi...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.02.005
更新日期：2017-08-01 00:00:00
abstract：:Clostridium difficile causes diarrhoeal diseases ranging from asymptomatic carriage to a fulminant, relapsing, and potentially fatal colitis. Endospore production plays a vital role in transmission of infection, and in order to cause disease these spores must then germinate and return to vegetative cell growth. Type B...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:Prevotella (formerly Bacteroides) ruminicola is a numerous rumen bacterium which plays a significant role in the metabolism of proteins and peptides in the rumen. Measurement of the hydrolysis of synthetic aminopeptidase substrates by sonicated extracts and whole cells of different species of rumen bacteria indicated ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1996.0065
更新日期：1997-02-01 00:00:00
abstract：:The use of gene therapy is one of the most recent molecular strategies for the treatment of cancer. It is essential, however, to have an efficient transfer system by which the desired gene can be delivered to the correct environment. The experiments described in this report investigate apathogenic Clostridium as a pos...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1998.0161
更新日期：1998-08-01 00:00:00
abstract：:The purpose of this study was to measure the effect of combined culture of Propionibacterium acnes and Staphylococcus aureus on biofilm formation under different oxygen concentrations. We measured planktonic growth and biofilm formation of P. acnes and S. aureus alone and together under aerobic and anaerobic condition...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.05.014
更新日期：2016-08-01 00:00:00
abstract：:Our goal was to establish a quantitative real-time PCR (QRT-PCR) method to detect Bacteroides fragilis group and related organisms from clinical specimens. Compared to conventional anaerobic culture, QRT-PCR can provide accurate and more rapid detection and identification of B. fragilis group and similar species. B. f...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.03.004
更新日期：2011-04-01 00:00:00
abstract：:Strain R1333, isolated from commercially available smoked salmon, was identified as Lactobacillus sakei based on biochemical tests, sugar fermentation reactions (API 50 CHL), PCR with species-specific primers and sequencing of the 16S rRNA gene. Strain R1333 produces a 3811 kDa class IIa bacteriocin, active against St...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.01.004
更新日期：2011-02-01 00:00:00
abstract：:Sourdough application has been extensively increased in the last years due to the consumers demand for food consumption without the addition of chemical preservatives. Several starter cultures have been applied in sourdough bread making targeting the increase of bread self-life and the improvement of sensorial charact...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.03.022
更新日期：2011-12-01 00:00:00
