Abstract:
:The bacteriolytic activity of nutritionally variant streptococci (NVS), fastidious microaerophilic bacteria, which are members of the genera Abiotrophia and Granulicatella, was characterized in a renaturating SDS polyacrylamide gel electrophoresis system. Bacteriolytic profiles appeared quite different for the three species of NVS examined. Granulicatella adiacens or Abiotrophia defectiva each presented at least seven lytic bands, four of which were in common, while the other three were species-specific, whereas Granulicatella elegans showed six bands, which were overlapping with the G. adiacens bands. Four lytic bands were identified for enzymatic activity; D-alanyl-L-lysine hydrolase, endo-N-acetylglucosaminidase, endoacetylmuramidase, D-glutamyl-L-lysine hydrolase and acetylmuramoyl-L-alanine amidase activities could be defined. The bacteriolytic enzymes were purified and characterized for the kinetics of production during growth, autolytic activity, temperature and pH stability.
journal_name
Anaerobejournal_title
Anaerobeauthors
Lampis G,Desogus A,Petruzzelli S,Laconi S,Ingianni A,Madeddu MA,Pompei Rdoi
10.1016/j.anaerobe.2007.08.002subject
Has Abstractpub_date
2007-10-01 00:00:00pages
238-43issue
5-6eissn
1075-9964issn
1095-8274pii
S1075-9964(07)00071-6journal_volume
13pub_type
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