Abstract：
:Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species. During stationary phase, these stains did not reflect physiological changes sufficiently and therefore additional labeling with bis-(1,3-dibutylbarbituric acid) trimethineoxonol was applied. Results of fluorescence staining in solventogenic batch fermentations were compared with substrate-use data, the concentration of key metabolites and growth curves. We demonstrate that measurements by all methods were mutually compatible.
journal_name
Anaerobejournal_title
Anaerobeauthors
Patakova P,Linhova M,Vykydalova P,Branska B,Rychtera M,Melzoch Kdoi
10.1016/j.anaerobe.2013.10.006subject
Has Abstractpub_date
2014-10-01 00:00:00pages
113-7eissn
1075-9964issn
1095-8274pii
S1075-9964(13)00173-Xjournal_volume
29pub_type
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