Abstract：
:A well-developed Golgi apparatus and rough and smooth endoplasmic reticulum in the principal cells of the mouse epididymis indicate active protein synthesis. Studies have shown that epididymal secretions are essential for sperm maturation. In a previous study, two wheat-germ agglutinin (WGA)-binding glycoproteins, GP-49 and GP-83, were identified on the surface of mature mouse sperm. In this study, synthesis and secretion of these two glycoproteins were investigated. Apparent WGA-binding was found on the stereocilia and in the apical region of principal cells in the corpus and cauda of epididymis. Post-fixation and pre-embedding cytochemical localization revealed that WGA-binding sites were situated in the Golgi apparatus, multivesicular bodies and stereocilia of principal cells. GP-49 and GP-83 were identified in the Nonidet P-40 homogenates of corpus and cauda epididymidis. In the epididymides of which ductuli efferentes had been ligated for more than 4 weeks, no sperm were found in the lumina of epididymal tubules. WGA-binding sites were present in the corpus and cauda; GP-49 and GP-83 were identified in tissue homogenates of the corpus and cauda as well. These findings suggest that GP-49 and GP-83 of mature sperm may be secreted by the principal cells of the corpus and cauda. These two molecules apparently conjugate to sperm whilst sperm transit through the epididymis.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Liu HW,Shang ST,Chao CF,Muller Cdoi
10.1007/BF00340863subject
Has Abstractpub_date
1991-09-01 00:00:00pages
409-14issue
3eissn
0302-766Xissn
1432-0878journal_volume
265pub_type
杂志文章abstract：:The fate of the major connective tissue polysaccharide hyaluronan, as it appears after release from the matrix, was studied in the Atlantic cod by use of subcutaneous administration of hyaluronan conjugated with fluorescein isothiocyanate (FITC) and labelled with 125I. After administration, the ligand was transported ...
journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1997-10-01 00:00:00
abstract：:Stereological studies showed that treatment of normal adult human adrenocortical cells in primary culture with ACTH or cyclic-AMP for 2 days results in similar increases in the volume of cells, of the mitochondrial and "membrane space" compartments and of the surface area of the smooth endoplasmic reticulum and mitoch...
journal_title：Cell and tissue research
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abstract：:The ultrastructure of crystalline beta granules of the islets of Langerhans in the alligator has been investigated. From optical diffraction analysis and serial sectioning, the existence of four distinct types of crystalline inclusions was established in ultrathin sections. The first type is the most frequent and is i...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00229618
更新日期：1978-03-13 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217308
更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：2000-07-01 00:00:00
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journal_title：Cell and tissue research
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更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2012-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222373
更新日期：1983-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004419900099
更新日期：1999-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2014-03-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410050833
更新日期：1997-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2342-1
更新日期：2016-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217273
更新日期：1982-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0827-5
更新日期：2009-09-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-019-03049-z
更新日期：2019-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219019
更新日期：1986-01-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224373
更新日期：1978-02-24 00:00:00
abstract：:The glucose transporter 8 (GLUT8) is a recently identified member of the family of sugar transport facilitators. In human tissues GLUT8 is predominantly expressed in testis in a gonadotropin-dependent manner. It is shown here that the onset of mRNA synthesis of GLUT8 during the maturation of mouse testis coincides wit...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-001-0499-2
更新日期：2002-02-01 00:00:00
abstract：:Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described t...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
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更新日期：2014-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1159-9
更新日期：2011-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
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更新日期：1998-06-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2844-8
更新日期：2018-10-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00306123
更新日期：1994-05-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220126
更新日期：1976-02-25 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219233
更新日期：1985-01-01 00:00:00
abstract：:A tissue-engineered heart valve can be an alternative to a prosthetic valve in heart valve replacement; however, it is not fully efficient in terms of long-lasting functionality, as leaflets in engineered valves do not possess the trilayered native leaflet structure. Previously, we developed a flat, trilayered, orient...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03241-6
更新日期：2020-11-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233959
更新日期：1980-01-01 00:00:00
abstract：:Immunoreactivity for calcitonin gene-related peptide is demonstrated for the first time in neuroepithelial bodies in the lung of newborn cats after Bouin fixation and embedding in paraffin. The intense staining clearly identifies these bodies at the level of bronchioli and alveoli. Occasionally, single neuroepithelial...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215517
更新日期：1987-08-01 00:00:00
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journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222332
更新日期：1985-01-01 00:00:00
