Abstract:
:While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causal agent of the most important arboviral diseases in this century. In this study, we successfully established a cell line stably expressing the precursor membrane/envelope (PrM/E) proteins of dengue virus type 2 (DENV2), which can package a DENV2 replicon with deletion of PrM/E genes and produce single-round infectious VLPs. Moreover, it can package a similar replicon of different serotype, dengue virus type 4, and produce infectious chimeric VLPs. To our knowledge, this study reports for the first time replicon-containing VLPs of dengue virus. Moreover, this convenient system has potential as a valuable tool to study encapsidation of dengue virus and to develop novel chimeric VLPs containing dengue virus replicon as vaccine in the future.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Lai CY,Hu HP,King CC,Wang WKdoi
10.1007/s11373-007-9204-0subject
Has Abstractpub_date
2008-01-01 00:00:00pages
15-27issue
1eissn
1021-7770issn
1423-0127journal_volume
15pub_type
杂志文章abstract::Developmentally regulated endothelial cell locus-1 (Del1) is an embryonic angiogenic factor expressed in early embryonic endothelial cells, but recently has been found to be expressed in some forms of cancers including colon and breast cancers, and melanoma, and human cancer cell lines. Overexpression of Del1 accelera...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-33
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:LncRNA Gas5 is known to be a key control element during growth, differentiation and development in mammalian species. However, the role and function of Gas5 in growth plate chondrocytes has not been determined. METHODS:The overexpression and knockdown models of Gas5 and miR-21 in cells and animals were cons...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0424-6
更新日期:2018-02-28 00:00:00
abstract::Schistosomiasis causes significant levels of morbidity and mortality in many geographical regions of the world. The disease is caused by infections with parasitic blood flukes known as schistosomes. The control of schistosomiasis over the last several decades has been centered on the mass drug administration (MDA) of ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-0621-y
更新日期:2020-01-22 00:00:00
abstract::The ACTGCTGA sequence (CTG motif) is located immediately upstream of the NF-kappaB enhancer in the human immunodeficiency virus type-1 (HIV-1) long terminal repeat (LTR). We previously reported on the frequent duplication of this motif in HIV-1-infected individuals. In this study we further characterized the role of t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02257981
更新日期:1994-03-01 00:00:00
abstract:INTRODUCTION:Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of epithelial-to-mesenc...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0511-3
更新日期:2019-02-13 00:00:00
abstract::A large group of small Rab GTPases which mediate secretory and endosomal membrane transport, as well as autophagosome biogenesis, are essential components of vesicle trafficking machinery. Specific Rab protein together with the cognate effectors coordinates the dynamics of trafficking pathway and determines the cargo ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0287-7
更新日期:2016-10-06 00:00:00
abstract::The HIV pol sequentially encodes protease (PR), reverse transcriptase (RT), and integrase (IN) from the 5'-3' direction. We explored the significance of this gene arrangement. All six possible gene dispositions were examined. In two situations where PR was removed from the leading place and no two genes were in their ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253522
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Despite the recent progress in screening and therapy, a majority of prostate cancer cases eventually attain hormone refractory and chemo-resistant attributes. Conventional chemotherapeutic strategies are effective at very high doses for only palliative management of these prostate cancers. Therefore chemo-se...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0127-1
更新日期:2015-04-01 00:00:00
abstract::Exposure of cells and organs to heat shock is associated with numerous changes in various cellular metabolic parameters and overexpression of proteins collectively known as heat shock proteins (HSP). In this communication we review the cell-signaling events that are altered in response to heat shock as they relate to ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258043
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:The association of TGF beta1 polymorphisms and atrial fibrillation (AF) in essential hypertensive (EH) subjects remains unknown. Methods EH subjects with AF (EH+AF+) and sinus rhythm (EH+AF-) were enrolled. The polymorphisms of +869 T --> C at codon 10 and + 915 G --> C at codon 25, were genotyped. The clini...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-23
更新日期:2010-03-31 00:00:00
abstract:BACKGROUND:Antibacterial activity is a novel function of high-mobility group box 1 (HMGB1). However, the functional site for this new effect is presently unknown. METHODS AND RESULTS:In this study, recombinant human HMGB1 A box and B box (rHMGB1 A box, rHMGB1 B box), recombinant human HMGB1 (rHMGB1) and the truncated ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-83
更新日期:2009-09-14 00:00:00
abstract:BACKGROUND:Ionizing radiation (IR) activate the early growth response-1 (Egr-1) promoter by production of radical oxygen intermediates (ROIs). Egr-EF, an expression vector pCIneo containing Egr-1 promoter cloned upstream of the cDNA for Flt3 ligand, was used to treat hematopoietic damage. 5-fluorouracil, a commonly use...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-85
更新日期:2009-09-21 00:00:00
abstract::Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy in terms of clinical features, underlying pathogenesis and treatment outcomes. Recent advances in genomic techniques have unraveled the molecular complexity of AML leukemogenesis, which in turn have led to refinement of risk stratification and pers...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00674-7
更新日期:2020-07-21 00:00:00
abstract:BACKGROUND:Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections....
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0472-y
更新日期:2018-09-18 00:00:00
abstract:BACKGROUND:Cisplatin (cis-diamminedichloroplatinum II, CDDP) is one of the most effective chemotherapeutic agents. However, its clinical use is limited due to the severe side effects, including nephrotoxicity and acute kidney injury (AKI) which develop due to renal accumulation and biotransformation of CDDP. The allevi...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0518-9
更新日期:2019-03-13 00:00:00
abstract:BACKGROUND:Lead (Pb2+) is an environmental neurotoxicant that disrupts neurodevelopment, communication, and organization through competition with Ca2+ signaling. How perinatal Pb2+ exposure affects Ca2+-related gene regulation remains unclear. However, Ca2+ activates the L-Type voltage sensitive calcium channel β-3 sub...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0450-4
更新日期:2018-05-24 00:00:00
abstract::Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256452
更新日期:2003-05-01 00:00:00
abstract::Acute respiratory distress syndrome (ARDS) can be associated with various disorders. Among these, coronavirus infection may cause life-threatening severe acute respiratory syndrome (SARS). In this review, we present animal models and techniques for the study of ARDS, and discuss the roles and possible mechanisms of va...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1159/000073523
更新日期:2003-01-01 00:00:00
abstract::The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectros...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9043-9
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. METHODS:Two normal, LB and HSC93, and two transformed, Ju...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-63
更新日期:2010-07-28 00:00:00
abstract:BACKGROUND:Adipogenesis is a complex process that involves many genes/proteins at different stages of differentiation. In order to identify genes critical for adipogenesis, we took a novel approach based on phenotype change of individual cell, to search for genes with regulatory roles in adipogenesis genome-wide in 3T3...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-92
更新日期:2009-10-13 00:00:00
abstract::Dehydroepiandrosterone (DHEA) is a putative anti-stress agent and stress is associated with the secretion of catecholamine from the adrenal gland, but the effects of DHEA on catecholamine secretion are not fully understood. Using bovine chromaffin cells, we found that DHEA inhibited catecholamine secretion and cytosol...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256563
更新日期:2004-03-01 00:00:00
abstract::The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9164-4
更新日期:2007-09-01 00:00:00
abstract:BACKGROUND:Prenatal alcohol exposure may cause cardiac development defects, however, the underlying mechanisms are not yet clear. In the present study we have investigated the roles of histone modification by curcumin on alcohol induced fetal cardiac abnormalities during the development. METHODS AND RESULTS:Q-PCR and ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-016-0310-z
更新日期:2017-01-05 00:00:00
abstract::Agents which block T cell K(+) currents can prohibit both proliferative and effector cell functions in T cells activated by mitogens or phorbol esters. This study examined the effects of some of these blocking agents on the immune responsiveness of guinea pig myelin basic protein (GPMBP)-reactive Lewis rat T lymphocyt...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255646
更新日期:1997-07-01 00:00:00
abstract::Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000079667
更新日期:2004-09-01 00:00:00
abstract::Sequencing of HIV-1(GER) long terminal repeat (LTR) has demonstrated, for the first time in an HIV-1 primary isolate, a TAR duplication referred to as TAR1 (nucleotides +1 through +68) and TAR2 (nucleotides +69 through +136). This TAR duplication is stable during replication of HIV-1(GER) isolate in CEM cells. Analysi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253576
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Glioma stem-like cells (GSCs) are proposed to be responsible for high resistance in glioblastoma multiforme (GBM) treatment. In order to find new strategies aimed at reducing GSC stemness and improving GBM patient survival, we investigated the effects and mechanism of a histone deacetylases (HDACs) inhibitor...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-016-0296-6
更新日期:2016-11-18 00:00:00
abstract:BACKGROUND:NKG2D, an activating immunoreceptor, is primarily restricted to NK cells and CD8(+) T cells. The existence of an atypical cytotoxic CD4(+)NKG2D(+) T cell population has also been found in patients with autoimmune dysfunctions. Nonetheless, contradictory evidence has categorized this population with a regulat...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0190-7
更新日期:2015-10-20 00:00:00
abstract::Influence of adjuvants i.e., alpha-tocopherol (25 mg/kg, p.o.) and piperine (10 mg/kg, p.o.) on therapeutic potential of chelator tiferron (300 mg/kg, i.p.) was evaluated to encounter toxicogenic events of beryllium exposure. Albino rats were exposed to beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9147-5
更新日期:2007-05-01 00:00:00