Abstract:
:Agents which block T cell K(+) currents can prohibit both proliferative and effector cell functions in T cells activated by mitogens or phorbol esters. This study examined the effects of some of these blocking agents on the immune responsiveness of guinea pig myelin basic protein (GPMBP)-reactive Lewis rat T lymphocytes, which are capable of mediating the adoptive transfer of experimental allergic encephalomyelitis (EAE), an accepted animal model for multiple sclerosis. Both the proliferative functions (DNA synthesis and cell blastogenesis) and the EAE transfer activities of GPMBP-reactive lymphocytes were examined following GPMBP-induced activation in the presence of agents shown to block the outwardly rectifying K(+) current in these cells. At concentrations which completely inhibited DNA synthesis, as measured by [(3)H]thymidine incorporation, and cell blastogenesis, tetraethylammonium (TEA), 4-aminopyridine (4-AP) and methoxyverapamil (D60) completely blocked the subsequent adoptive transfer of EAE into naive syngeneic Lewis rats. The concentrations at which these blockers produced a 50% reduction in DNA synthesis were estimated to be 16, 1.6 and 32 &mgr;M for TEA, 4-AP and D-600, respectively, which were roughly equivalent to the EC(50) to block the K(+) current. Apamine, a potent Ca(2+)-activated K(+) channel blocker, at a concentration several orders of magnitude higher than is necessary to block Ca(2+)-activated K(+) channels, reduced the maximal K(+) conductance in GPMBP-reactive T cell K(+) channels by about 20%, but did not alter either [H(3)H]thymidine incorporation or the adoptive transfer of EAE. These results indicate that delayed rectifier K(+) channel blockers may prevent the activation of GPMBP-reactive T cells, thus prohibiting encephalitogenic effector cell functions. Copyright 1997 S. Karger AG, Basel
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Judge SI,Yeh JZ,Mannie MD,Pope Seifert L,Paterson PYdoi
10.1007/BF02255646keywords:
subject
Has Abstractpub_date
1997-07-01 00:00:00pages
169-178issue
4eissn
1021-7770issn
1423-0127pii
jbs04169journal_volume
4pub_type
杂志文章abstract::Glucocorticoids regulate the expression of many liver-specific genes via glucocorticoid receptors. The presence of glucocorticoid receptors in liver has been reported in many mammalian species but not in nude mice. In the present study, we demonstrate the presence of specific glucocorticoid receptors in nude mouse liv...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253306
更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255905
更新日期:1999-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256609
更新日期:2001-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Journal of biomedical science
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doi:10.1186/s12929-015-0130-6
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-18-78
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S34
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
doi:10.1186/s12929-017-0318-z
更新日期:2017-01-31 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-0624-8
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9139-x
更新日期:2007-03-01 00:00:00
abstract::Oxidative stress and inflammation contributed to the propagation of acute liver injury (ALI). The present study was undertaken to determine whether D-galactosamine (D-GalN) induces ALI via the mitochondrial apoptosis- and proinflammatory cytokine-signaling pathways, and possible mechanism(s) by which green tea (GT) ex...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-35
更新日期:2009-03-25 00:00:00
abstract::Induction of tumoricidal activity is one of the major functions of activated macrophages. Our previous study demonstrated that P388D1 murine macrophage-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent stu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255919
更新日期:2000-01-01 00:00:00
abstract::Phospholipase D (PLD) is a phosphodiesterase that catalyses hydrolysis of phosphatidylcholine to produce phosphatidic acid and choline. In the presence of ethanol, PLD also catalyses the formation of phosphatidylethanol, which is a unique characteristic of this enzyme. Muscarinic receptor-induced changes in the activi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256440
更新日期:1999-03-01 00:00:00
abstract::Due to high prevalence and mortality and the lack of effective therapies, prostate cancer is one of the most crucial health problems in men. Drug resistance aggravates the situation, not only in human prostate cancer but also in other cancers. In this study, we report for the first time that cardiac glycosides (e.g. o...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256539
更新日期:2002-09-01 00:00:00
abstract::A number of infectious agents have been implicated in the development of vascular diseases such as atherosclerosis and posttransplantation arterial restenosis. Cytomegalovirus (CMV) has been reported to cause obliteration of coronary arteries by a progressive vasculopathy that involves proliferation of medial smooth m...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256111
更新日期:2003-09-01 00:00:00
abstract::The interactive role of rostral ventrolateral medulla (RVL) cardiovascular neurons and brain angiotensin II (Ang II) in regulating the arterial blood pressure was examined by recording simultaneously the spontaneous activity of these spinal projecting neurons and the arterial blood pressure in the pentobarbital-anesth...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253100
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Parkinson's disease is the second most common neurodegenerative disorders after Alzheimer's disease. The main cause of the disease is the massive degeneration of dopaminergic neurons in the substantia nigra. Neuronal apoptosis and neuroinflammation are thought to be the key contributors to the neuronal degen...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0146-y
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
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doi:10.1186/1423-0127-21-44
更新日期:2014-05-13 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00654-x
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journal_title:Journal of biomedical science
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-20-34
更新日期:2013-05-31 00:00:00
abstract:BACKGROUND:Helicobacter pylori bacteria colonize human gastric mucosa, cause chronic inflammation, peptic ulcers and gastric cancer. Colonization is mediated by H. pylori adhesins, which preferentially bind mucin 5 (MUC5AC) and Lewis (Le) determinants. The aim of this study was to evaluate the influence of H. pylori an...
journal_title:Journal of biomedical science
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doi:10.1186/s12929-019-0515-z
更新日期:2019-03-06 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S43
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255935
更新日期:1998-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:2020-04-02 00:00:00