Abstract:
:After streptococcal pyrogenic exotoxin B (SPE B) induces apoptosis, its fate is unknown. Using confocal time-course microscopy at 37 degrees C, we detected green fluorescence 20 min after adding FITC-SPE B. Orange fluorescence, an indication of co-localization of SPE B with lysosomes which were labeled with a red fluorescent probe, was maximal at 40 min and absent by 60 min. SPE B was co-precipitated with clathrin, which is consistent with endocytotic involvement. Western blotting assay also indicated that uptake of SPE B was maximal at 40 min and disappeared after 60 min. However, in the presence of chloroquine, a lysosome inhibitor, the uptake of SPE B was not detectable. The disappearance of TCA-precipitated FITC-SPE B was parallel to the appearance of TCA soluble FITC-SPE B; in the presence of chloroquine, however, no SPE B degradation occurred. Chloroquine increased the level of SPE B-induced apoptosis by inhibiting the degradation of SPE B. These results suggest that the internalization and degradation of SPE B in cells may be a host defense system that removes toxic substances by sacrificing the exposed cells.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chang CW,Tsai WH,Chuang WJ,Lin YS,Wu JJ,Liu CC,Tsai PJ,Lin MTdoi
10.1007/s11373-007-9154-6subject
Has Abstractpub_date
2007-05-01 00:00:00pages
419-27issue
3eissn
1021-7770issn
1423-0127journal_volume
14pub_type
杂志文章abstract::Microfluidics has become an important tool in diagnosing many diseases, including neurological and genetic disorders. Alzheimer's disease (AD) is a neurodegenerative disease that irreversibly and progressively destroys memory, language ability, and thinking skills. Commonly, detection of AD is expensive and complex. F...
journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Journal of biomedical science
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doi:10.1007/BF02255469
更新日期:2000-05-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2004-11-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2003-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,多中心研究
doi:10.1186/1423-0127-19-1
更新日期:2012-01-03 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255905
更新日期:1999-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258357
更新日期:1997-11-01 00:00:00
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doi:10.1186/1423-0127-18-26
更新日期:2011-04-15 00:00:00
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
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更新日期:2009-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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更新日期:2020-05-05 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2006-07-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2015-10-15 00:00:00
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更新日期:2013-11-19 00:00:00
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journal_title:Journal of biomedical science
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doi:10.1007/BF02253428
更新日期:1997-01-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2001-01-01 00:00:00