Abstract：
:An isolated thoracic spinal cord of the neonatal rat in vitro spontaneously generates sympathetic nerve discharge (SND) at ~25 degrees C, but it fails in SND genesis at < or = 10 degrees C. Basal levels of the c-Fos expression in the spinal cords incubated at < or = 10 degrees C and ~25 degrees C were compared to determine the anatomical substrates that might participate in SND genesis. Cells that exhibited c-Fos immunoreactivity were virtually absent in the spinal cords incubated at < or = 10 degrees C. However, in the spinal cords incubated at ~25 degrees C, c-Fos-positive cells were found in the dorsal laminae, the white matter, lamina X, and the intermediolateral cell column (IML). Cell identities were verified by double labeling of c-Fos with neuron-specific nuclear protein (NeuN), glial fibrillary acidic protein (GFAP), or choline acetyltransferase (ChAT). The c-Fos-positive cells distributed in the white matter and lamina X were NeuN-negative or GFAP-positive and were glial cells. Endogenously active neurons showing c-Fos and NeuN double labeling were scattered in the dorsal laminae and concentrated in the IML. Double labeling of c-Fos and ChAT confirmed the presence of active sympathetic preganglionic neurons (SPNs) in the IML. Suppression of SND genesis by tetrodotoxin (TTX) or mecamylamine (MECA, nicotinic receptor blocker) almost abolished c-Fos expression in dorsal laminae, but only mildly affected c-Fos expression in the SPNs. Therefore, c-Fos expression in some SPNs does not require synaptic activation. Our results suggest that spinal SND genesis is initiated from some spontaneously active SPNs, which are capable of TTX- or MECA-resistant c-Fos expression.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Su CK,Ho CM,Kuo HH,Wen YC,Chai CYdoi
10.1186/1423-0127-16-44subject
Has Abstractpub_date
2009-05-01 00:00:00pages
44eissn
1021-7770issn
1423-0127pii
1423-0127-16-44journal_volume
16pub_type
杂志文章abstract：:Plasma nitric oxide (NO) levels in Duchenne muscular dystrophy (DMD) patients were significantly lower than those observed in both healthy controls and in patients with other neuromuscular disorders. The correlation between NO level and ejection fraction was significant (r = -0.384, p = 0.0391) in the DMD group. Disru...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256104
更新日期：2004-07-01 00:00:00
abstract：:Phospholipase D (PLD) is a phosphodiesterase that catalyses hydrolysis of phosphatidylcholine to produce phosphatidic acid and choline. In the presence of ethanol, PLD also catalyses the formation of phosphatidylethanol, which is a unique characteristic of this enzyme. Muscarinic receptor-induced changes in the activi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256440
更新日期：1999-03-01 00:00:00
abstract：BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0602-1
更新日期：2020-02-08 00:00:00
abstract：BACKGROUND:Detection of cholangiocarcinoma (CCA) remains a diagnostic challenge. We established diagnostic peptide biomarkers in bile and urine based on capillary electrophoresis coupled to mass spectrometry (CE-MS) to detect both local and systemic changes during CCA progression. In a prospective cohort study we recen...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0599-5
更新日期：2020-01-03 00:00:00
abstract：:The nuclear envelope (NE) consists of two membrane layers that segregate the nuclear from the cytoplasmic contents. Recent progress in our understanding of nuclear-lamina associated diseases has revealed intriguing connections between the envelope components and nuclear processes. Here, we review the functions of the ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/1423-0127-16-96
更新日期：2009-10-22 00:00:00
abstract：:The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-004-8166-8
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Honokiol, a cell-permeable phenolic compound derived from the bark of magnolia trees and present in Asian herbal teas, has a unique array of pharmacological actions, including the inhibition of multiple autonomic responses. We determined the effects of honokiol on calcium signaling underlying transmission me...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-20-11
更新日期：2013-02-23 00:00:00
abstract：BACKGROUND:Oxidized LDL (oxLDL) is involved in the development of atherosclerotic heart disease through a mechanism that is not fully understood. In this study, we examined the role of malondialdehyde (MDA), an important oxidative stress epitope of oxLDL, in mediating coronary endothelial cytotoxicity. RESULTS:Human c...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-11
更新日期：2014-02-03 00:00:00
abstract：:Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256452
更新日期：2003-05-01 00:00:00
abstract：:Sensory innervation of the skin subserves protective sensations for the body to prevent thermal and noxious injuries. Neurophysiologically, they belong to the categories of Adelta and C fibers, usually with caliber less than one micro m in diameter. Morphological demonstration of the terminals of these nerves in the e...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253428
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Noopept (N-phenyl-acetyl-L-prolylglycine ethyl ester) was constructed as a dipeptide analog of the standard cognition enhancer, piracetam. Our previous experiments have demonstrated the cognition restoring effect of noopept in several animal models of Alzheimer disease (AD). Noopept was also shown to prevent...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-014-0074-2
更新日期：2014-08-06 00:00:00
abstract：BACKGROUND:Sensing tissue acidosis is an important function of the somatosensory nervous system to response to noxious stimuli. MAIN BODY:In the pain clinic, acid or soreness sensation is a characteristic sensory phenotype of various acute and chronic pain syndromes, such as delayed onset muscle soreness, fibromyalgia...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0486-5
更新日期：2018-11-29 00:00:00
abstract：:In all eukaryotes, the plasma membrane is critically important as it maintains the architectural integrity of the cell. Proper anchorage and interaction between the plasma membrane and the cytoskeleton is critical for normal cellular processes. The ERM (ezrin-radixin-moesin) proteins are a class of highly homologous p...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0246-3
更新日期：2016-03-17 00:00:00
abstract：BACKGROUND:The blood-spinal cord barrier (BSCB) is composed of a monolayer of endothelium linked with tight junctions and extracellular matrix (ECM)-rich basement membranes and is surrounded by astrocyte foot processes. Endothelial permeability is regulated by interaction between endothelial cells and ECM proteins. Fib...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0529-6
更新日期：2019-05-16 00:00:00
abstract：BACKGROUND:Prenatal alcohol exposure may cause cardiac development defects, however, the underlying mechanisms are not yet clear. In the present study we have investigated the roles of histone modification by curcumin on alcohol induced fetal cardiac abnormalities during the development. METHODS AND RESULTS:Q-PCR and ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0310-z
更新日期：2017-01-05 00:00:00
abstract：BACKGROUNDS:Homozygous 32-bp deletion of the chemokine receptor 5 gene (CCR5) is associated with resistance to human immunodeficiency virus (HIV) infection, while heterozygosity delays HIV progression. Bone marrow transplantation (BMT) from a 32/32 donor has been shown to cure an HIV-infected patient. However, the rari...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0130-6
更新日期：2015-03-26 00:00:00
abstract：:Reactive oxygen species (ROS) have been long considered simply as harmful by-products of metabolism, which damage cellular proteins, lipids, and nucleic acids. ROS are also known as a weapon of phagocytes, employed against pathogens invading the host. However, during the last decade, an understanding has emerged that ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-015-0194-3
更新日期：2015-10-15 00:00:00
abstract：BACKGROUND:Glutaminase predominates in periportal hepatocytes and it has been proposed that it determines the glutamine-derived nitrogen flow through the urea cycle. Glutamine-derived urea production should, thus, be considerably faster in periportal hepatocytes. This postulate, based on indirect observations, has not ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-1
更新日期：2010-01-07 00:00:00
abstract：:The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256432
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND:Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. METHODS AND RESULTS:MSCs were treated with...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9207-x
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:To mimic systemic inflammation in humans, different animal models have been developed. Since these models are still discussed controversially, we aimed to comparatively evaluate the most widely used models with respect to the systemic effects, the influence on organ functions and to the underlying pathophysi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0370-8
更新日期：2017-08-24 00:00:00
abstract：:Drosophila eye development is a progressive process including cell fate determination, pattern formation, and rhabdomere morphogenesis. During eye development, a dramatic change in cell shape, which involves turning and extension of the photoreceptor apical surface, occurs in the early pupal stages. It is known that a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02254441
更新日期：2004-05-01 00:00:00
abstract：:Recent transcriptome analyses have revealed that noncoding RNAs (ncRNAs) are broadly expressed in mammalian cells and abundant in the CNS, with tissue and cell type-specific expression patterns. Moreover, ncRNAs have been found to intricately and dynamically regulate various signaling pathways in neurodegeneration. As...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00636-z
更新日期：2020-04-07 00:00:00
abstract：BACKGROUND:Autophagy has been shown recently to play an important role in the intracellular survival of several pathogenic bacteria. In this study, we investigated the effect of a novel small-molecule autophagy-inducing agent, AR-12, on the survival of Francisella tularensis, the causative bacterium of tularemia in hum...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-110
更新日期：2009-12-09 00:00:00
abstract：:Ischemia and reperfusion (I/R) - induced injury has been described as one of the main factors that contribute to the observed morbidity and mortality in a variety of clinical entities, including myocardial infarction, ischemic stroke, cardiac arrest and trauma. An imbalance between oxygen demand and supply, within the...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0220-0
更新日期：2016-01-19 00:00:00
abstract：:Oxidative stress and inflammation contributed to the propagation of acute liver injury (ALI). The present study was undertaken to determine whether D-galactosamine (D-GalN) induces ALI via the mitochondrial apoptosis- and proinflammatory cytokine-signaling pathways, and possible mechanism(s) by which green tea (GT) ex...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-35
更新日期：2009-03-25 00:00:00
abstract：:Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000079667
更新日期：2004-09-01 00:00:00
abstract：:Experimental allergic encephalomyelitis (EAE) is an accepted animal model for the human demyelinating disease multiple sclerosis. The continuously propagated line of Lewis rat T helper lymphocytes (GP1 T cells), specific for the encephalitogenic 68-86 sequence of guinea pig myelin basic protein (GPMBP), mediates the a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255600
更新日期：1997-03-01 00:00:00
abstract：BACKGROUND:Whereas brain death is a vitally important clinical phenomenon, our contemporary understanding on its underlying cellular mechanisms remains elusive. This study evaluated whether the ubiquitin-proteasome system (UPS) in the rostral ventrolateral medulla (RVLM), a neural substrate that our laboratory identifi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-48
更新日期：2012-04-30 00:00:00
abstract：:Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy in terms of clinical features, underlying pathogenesis and treatment outcomes. Recent advances in genomic techniques have unraveled the molecular complexity of AML leukemogenesis, which in turn have led to refinement of risk stratification and pers...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00674-7
更新日期：2020-07-21 00:00:00
