Abstract：
:The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from a medulloblastoma. This protein, also named Ciz1, has previously been characterized for its ability to interact with p21(Cip1/Waf1) and contains 3 zinc finger domains and a matrin 3-homologous domain 3. Our immunofluorescence and Northern blot analysis data indicate that Ciz1 is localized in the nucleus and is expressed in a wide range of tissues, especially the pancreas and the brain; within the brain, the highest message levels are found in the cerebellum. A modified selected and amplified binding (SAAB) sequence method was used to identify DNA sequences recognized by Ciz1. From the analysis of the retrieved SAAB sequences and verification using electrophoretic mobility shift assays, we formulated a consensus DNA sequence, ARYSR(0-2)YYAC, recognized by Ciz1. The potential activities of Ciz1, including those involved in brain tumorigenesis, are discussed.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Warder DE,Keherly MJdoi
10.1007/BF02256432keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
406-17issue
4eissn
1021-7770issn
1423-0127pii
71160journal_volume
10pub_type
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journal_title：Journal of biomedical science
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更新日期：2001-01-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
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更新日期：2001-03-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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journal_title：Journal of biomedical science
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