Abstract:
:Activation of hepatic stellate cells (HSCs) plays a crucial role in liver fibrogenesis. armepavine (Arm, C19H23O3N), an active compound from Nelumbo nucifera, has been shown to exert immunosuppressive effects on T lymphocytes and on lupus nephritic mice. The aim of this study was to investigate whether Arm could exert anti-hepatic fibrogenic effects in vitro and in vivo. A cell line of rat HSCs (HSC-T6) was stimulated with tumor necrosis factor-alpha (TNF-alpha) or lipopolysaccharide (LPS) to evaluate the inhibitory effects of Arm. An in vivo therapeutic study was conducted in bile duct-ligated (BDL) rats. BDL rats were given Arm (3 or 10 mg/kg) by gavage twice daily for 3 weeks starting from the onset of BDL. Liver sections were taken for fibrosis scoring, immuno-fluorescence staining and quantitative real-time mRNA measurements. In vitro, Arm (1-10 microM) concentration-dependently attenuated TNF-alpha- and LPS-stimulated alpha-SMA protein expression and AP-1 activation by HSC-T6 cells without adverse cytotoxicity. Arm also suppressed TNF-alpha-induced collagen collagen deposition, NFkappaB activation and MAPK (p38, ERK1/2, and JNK) phosphorylations. In vivo, Arm treatment significantly reduced plasma AST and ALT levels, hepatic alpha-SMA expression and collagen contents, and fibrosis scores of BDL rats as compared with vehicle treatment. Moreover, Arm attenuated the mRNA expression levels of col 1alpha2, TGF-beta1, TIMP-1, ICAM-1, iNOS, and IL-6 genes, but up-regulated metallothionein genes. Our study results showed that Arm exerted both in vitro and in vivo antifibrotic effects in rats, possibly through anti-NF-kappaB activation pathways.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Weng TC,Shen CC,Chiu YT,Lin YL,Kuo CD,Huang YTdoi
10.1186/1423-0127-16-78subject
Has Abstractpub_date
2009-09-02 00:00:00pages
78eissn
1021-7770issn
1423-0127pii
1423-0127-16-78journal_volume
16pub_type
杂志文章abstract::Retrograde transport of Wheat germ agglutinin conjugated to Horseradish peroxidase (WGA-HRP) was used in labeling vagal neurons projecting to the stomach from the dorsal motor nucleus of the vagus nerve (DMNV) in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in the experimental rats by intraperitone...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9097-3
更新日期:2006-09-01 00:00:00
abstract::The nucleotide sequences of the putative envelope region (E1) and the junction between the E1 and envelope 2/nonstructural 1 (E2/NS1) region of the hepatitis C virus (HCV) genome are divergent among different genotypes. To characterize them, we introduced a set of nested primers that are conserved among four different...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253343
更新日期:1994-06-01 00:00:00
abstract::The present study was to investigate the effects and action mechanisms of dehydroepiandrosterone (DHEA) on steroidogenesis in rat adrenal zona glomerulosa cells (ZG). ZG cells were incubated with DHEA in the presence or absence of angiotensin II (AngII), a high concentration of potassium, 8-Br-cAMP, forskolin, 25-OH-c...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-008-9241-3
更新日期:2008-07-01 00:00:00
abstract::The relationship between diet and colorectal cancer has been previously demonstrated and supported with strong epidemiological evidence. The role of genetic polymorphisms has, however, been less well elaborated upon. We conducted a hospital-based case-control study including 727 cases and 736 healthy controls to evalu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9139-x
更新日期:2007-03-01 00:00:00
abstract::Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/s11373-005-9063-5
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Cell adhesion has been shown to induce activation of certain growth factor receptors in a ligand-independent manner. However, the mechanism for such activation remains obscure. METHODS:Human epidermal carcinoma A431 cells were used as a model to examine the mechanism for adhesion-induced activation of hepat...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-18-78
更新日期:2011-10-27 00:00:00
abstract:BACKGROUND:The beta-amino acid, taurine, is a nutritional requirement in some species. In these species, the depletion of intracellular stores of taurine leads to the development of severe organ dysfunction. The basis underlying these defects is poorly understood, although there is some suggestion that oxidative stress...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S25
更新日期:2010-08-24 00:00:00
abstract::As a neurotropic virus, enterovirus A71 (EV-A71) emerge and remerge in the Asia-Pacific region since the 1990s, and has continuously been a threat to global public health, especially in children. Annually, EV-A71 results in hand-foot-and-mouth disease (HFMD) and occasionally causes severe neurological disease. Here we...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0574-1
更新日期:2019-10-21 00:00:00
abstract:BACKGROUND:Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. METHODS:Two normal, LB and HSC93, and two transformed, Ju...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-63
更新日期:2010-07-28 00:00:00
abstract:BACKGROUND:Promoting post-stroke neurogenesis has long been proposed to be a therapeutic strategy for the enhancement of functional recovery after cerebral ischemic stroke. Despite numerous approaches have been widely reported the proliferation or differentiation of the neurogenic population therapeutic strategies by t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0521-1
更新日期:2019-04-18 00:00:00
abstract::The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9164-4
更新日期:2007-09-01 00:00:00
abstract::The interactive role of rostral ventrolateral medulla (RVL) cardiovascular neurons and brain angiotensin II (Ang II) in regulating the arterial blood pressure was examined by recording simultaneously the spontaneous activity of these spinal projecting neurons and the arterial blood pressure in the pentobarbital-anesth...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253100
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:The blood-spinal cord barrier (BSCB) is composed of a monolayer of endothelium linked with tight junctions and extracellular matrix (ECM)-rich basement membranes and is surrounded by astrocyte foot processes. Endothelial permeability is regulated by interaction between endothelial cells and ECM proteins. Fib...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0529-6
更新日期:2019-05-16 00:00:00
abstract:BACKGROUNDS:Homozygous 32-bp deletion of the chemokine receptor 5 gene (CCR5) is associated with resistance to human immunodeficiency virus (HIV) infection, while heterozygosity delays HIV progression. Bone marrow transplantation (BMT) from a 32/32 donor has been shown to cure an HIV-infected patient. However, the rari...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0130-6
更新日期:2015-03-26 00:00:00
abstract:BACKGROUND:The association between ischemic stroke and 2 single nucleotide polymorphisms (SNPs) on chromosome 12p13, rs12425791 and rs11833579 appears inconsistent across different samples. These SNPs are close to the ninjurin2 gene which may alter the risk of stroke by affecting brain response to ischemic injury. The ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,多中心研究
doi:10.1186/1423-0127-19-1
更新日期:2012-01-03 00:00:00
abstract:BACKGROUND:Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine and elevated in the regions of tissue injury and inflammatory diseases. The deleterious effects of TNF-α on fibroblasts may aggravate heart inflammation mediated through the up-regulation of adhesion molecules such as vascular cell adhesion molecu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0165-8
更新日期:2015-07-15 00:00:00
abstract::Alterations in eIF3-p48/INT6 gene expression have been implicated in murine and human mammary carcinogenesis. We examined levels of INT6 protein in human tumors and determined that breast and colon tumors clustered into distinct groups based on levels of INT6 expression and clinicopathological variables. We performed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9149-3
更新日期:2007-05-01 00:00:00
abstract::Increasing evidence suggests that immune responses are involved in the control of cancer and that the immune system can be manipulated in different ways to recognize and attack tumors. Progress in immune-based strategies has opened new therapeutic avenues using a number of techniques destined to eliminate malignant ce...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/1423-0127-16-30
更新日期:2009-03-06 00:00:00
abstract::While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causa...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9204-0
更新日期:2008-01-01 00:00:00
abstract::To identify novel genes associated with apoptosis in glioma cells, we treated T98G glioma cells with okadaic acid (OA). Differential display using 15 random primers was performed on RNA extracted from these cells. Upregulated bands were excised from polyacrylamide gels and cloned. Northern blots were used to confirm R...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256622
更新日期:2000-03-01 00:00:00
abstract::The Peutz-Jeghers syndrome (PJS) is a hereditary disorder that predisposes an individual to benign and malignant tumors in multiple organ systems. Recently, the locus responsible for PJS was mapped genetically to the LKB1 gene, with a subsequent investigation proving that it is responsible for most cases of PJS. LKB1 ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256059
更新日期:2003-03-01 00:00:00
abstract::Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256452
更新日期:2003-05-01 00:00:00
abstract::Glycine N-methyltransferase (GNMT) is a protein with multiple functions. Recently, two Italian siblings who had hepatomegaly and chronic elevation of serum transaminases were diagnosed to have GNMT deficiency caused by inherited compound heterozygosity of the GNMT gene with missence mutations. To evaluate the expressi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256001
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The diastolic oscillatory after-potential Vos and pre-potential ThVos play an essential role in the pacemaker mechanism of sino-atrial node (SAN). The aim of this study was to investigate whether these oscillatory potentials are also involved in adrenergic control of SAN discharge. METHODS:Vos and ThVos wer...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-101
更新日期:2009-11-18 00:00:00
abstract::Mutations at codon 215 of HIV-1 reverse transcriptase (RT) confer resistance to nucleoside analogs through RT-catalyzed ATP-dependent phosphorolysis. We showed that mutation T215Y is predominant over T215F (respectively 38.8 vs. 7.04% of 7312 sequences from a cohort of patients receiving antiretroviral therapy in Fran...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9011-4
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:Chemotherapy and targeted therapies have made important strides in cancer treatment yet they often fail and new therapies are still needed. Here, we employed a phenotypic screen to identify and analyze the mechanism of action of novel small molecules that interfere with critical pathways involved in tumor ce...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0408-6
更新日期:2018-02-08 00:00:00
abstract:BACKGROUND:Fat embolism syndrome (FES) associated with acute lung injury (ALI) is a clinical condition following long bone fracture. We have reported 14 victims due to ALI with FES. Our laboratory has developed an animal model that produced fat emboli (FE). The major purpose of this study was to test whether neutrophil...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-3
更新日期:2012-01-05 00:00:00
abstract::Obesity is one of the most invaliding and preventable diseases in the United States. Growing evidence suggests that there are sex differences in obesity in human and experimental animals. However, the specific mechanisms of this disease are unknown. Consequently, there is any particular treatment according to the sex/...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-018-0488-3
更新日期:2018-12-03 00:00:00
abstract:BACKGROUND:Developing brain is a major target for alcohol's actions and neurological/functional abnormalities include microencephaly, reduced frontal cortex, mental retardation and attention-deficits. Previous studies have shown that ethanol altered the lateral ventricular neuroepithelial cell proliferation. However, t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-016-0225-8
更新日期:2016-01-19 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Journal of biomedical science
pub_type: 已发布勘误
doi:10.1186/s12929-020-00686-3
更新日期:2020-09-21 00:00:00