当前位置： SCI文献检索 > JOURNAL OF BIOMEDICAL SCIENCE期刊下所有文献 > Characterization of reduced expression of glycine N-methyltransferase in cancerous hepatic tissues using two newly developed monoclonal antibodies.

Characterization of reduced expression of glycine N-methyltransferase in cancerous hepatic tissues using two newly developed monoclonal antibodies.

Abstract：

:Glycine N-methyltransferase (GNMT) is a protein with multiple functions. Recently, two Italian siblings who had hepatomegaly and chronic elevation of serum transaminases were diagnosed to have GNMT deficiency caused by inherited compound heterozygosity of the GNMT gene with missence mutations. To evaluate the expression of GNMT in cell lines and tissues from hepatocellular carcinoma (HCC) patients, we produced two monoclonal antibodies (mAbs) 4-17 and 14-1 using two recombinant GNMT fusion proteins. M13 phage peptide display showed that the reactive epitopes of mAbs 4-17 and 14-1 were amino acid residues 11-15 and 272-276 of human GNMT, respectively. The dissociation constants of the binding between GNMT and mAbs were 1.7 x 10(-8) M for mAb 4-17 and 1.8 x 10(-9) M for mAb 14-1. Both mAbs can identify GNMT present in normal human and mouse liver tissues using Western blotting (WB) and immunohistochemical staining assay (IHC). In addition, WB with both mAbs showed that none of 2 hepatoblastoma and 5 HCC cell lines expressed GNMT. IHC demonstrated that 50% (13/26) of nontumorous liver tissues and 96% (24/25) of HCC tissues did not express GNMT. Therefore, the expression of GNMT was downregulated in human HCC.

journal_name

J Biomed Sci

journal_title

Journal of biomedical science

authors

Liu HH,Chen KH,Shih YP,Lui WY,Wong FH,Chen YM

doi

10.1007/BF02256001

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

87-97

issue

1

eissn

1021-7770

issn

1423-0127

pii

68083

journal_volume

10

pub_type

杂志文章

相关文献

JOURNAL OF BIOMEDICAL SCIENCE文献大全
  • In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties.

    abstract：BACKGROUND:Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certain inflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, our goal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties....

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-17-8

    authors： Luquita A,Urli L,Svetaz MJ,Gennaro AM,Giorgetti ME,Pistone G,Volpintesta R,Palatnik S,Rasia M

    更新日期：2010-02-12 00:00:00

  • Incorporation of dengue virus replicon into virus-like particles by a cell line stably expressing precursor membrane and envelope proteins of dengue virus type 2.

    abstract：:While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causa...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-007-9204-0

    authors： Lai CY,Hu HP,King CC,Wang WK

    更新日期：2008-01-01 00:00:00

  • Diazoxide ameliorates microcirculatory disturbances through PKC-dependent pathway in I/R-injured rat cremaster muscles.

    abstract：:Diazoxide is a selective mitochondria ATP-sensitive potassium (K(ATP)) channel opener, which has been reported to preserve the microvascular integrity of ischemia-reperfusion (I/R)-injured tissues. Our study aimed to assess diazoxide's effects on I/R-injured cremaster muscles and to further elucidate its underlying me...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-005-3730-4

    authors： Wei W,Wei FC,Hung LM

    更新日期：2005-01-01 00:00:00

  • Dynactin affects extension and assembly of adherens junctions in Drosophila photoreceptor development.

    abstract：:Drosophila eye development is a progressive process including cell fate determination, pattern formation, and rhabdomere morphogenesis. During eye development, a dramatic change in cell shape, which involves turning and extension of the photoreceptor apical surface, occurs in the early pupal stages. It is known that a...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02254441

    authors： Fan SS

    更新日期：2004-05-01 00:00:00

  • Structure and Activity of Membrane Receptors: Modeling and Computational Simulation of Ligand Recognition in a Three-Dimensional Model of the 5-Hydroxytryptamine(1A) Receptor.

    abstract：:A three-dimensional molecular model of the transmembrane domain of the 5-HT(1A) receptor (5-HT(1A)R) is presented in the context of a general strategy for modeling the macromolecular structure of a guanine nucleotide binding, regulatory protein coupled receptor (GPCR). The model of the 5-HT(1A)R rests on the definitio...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02255537

    authors： Pardo L,Batlle M,Duñach M,Weinstein H

    更新日期：1996-04-01 00:00:00

  • Isoform-specific effects of apoE on neurite outgrowth in olfactory epithelium culture.

    abstract：BACKGROUND:The apolipoprotein E4 (apoE4) genotype is a major risk factor for developing late-onset Alzheimer's disease (AD). Inheritance of apoE4 is also associated with impairments in olfactory function in early stages of AD. In this project we examined the effects of the three common isoforms of human apoE (apoE2, ap...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-20-49

    authors： Hussain A,Luong M,Pooley A,Nathan BP

    更新日期：2013-07-12 00:00:00

  • Molecular mechanisms of cisplatin-induced nephrotoxicity: a balance on the knife edge between renoprotection and tumor toxicity.

    abstract：BACKGROUND:Cisplatin (cis-diamminedichloroplatinum II, CDDP) is one of the most effective chemotherapeutic agents. However, its clinical use is limited due to the severe side effects, including nephrotoxicity and acute kidney injury (AKI) which develop due to renal accumulation and biotransformation of CDDP. The allevi...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/s12929-019-0518-9

    authors： Volarevic V,Djokovic B,Jankovic MG,Harrell CR,Fellabaum C,Djonov V,Arsenijevic N

    更新日期：2019-03-13 00:00:00

  • Structural analysis of reverse transcriptase mutations at codon 215 explains the predominance of T215Y over T215F in HIV-1 variants selected under antiretroviral therapy.

    abstract：:Mutations at codon 215 of HIV-1 reverse transcriptase (RT) confer resistance to nucleoside analogs through RT-catalyzed ATP-dependent phosphorolysis. We showed that mutation T215Y is predominant over T215F (respectively 38.8 vs. 7.04% of 7312 sequences from a cohort of patients receiving antiretroviral therapy in Fran...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-005-9011-4

    authors： Yahi N,Fantini J,Henry M,Tourrès C,Tamalet C

    更新日期：2005-10-01 00:00:00

  • TGF-β induces HLA-G expression through inhibiting miR-152 in gastric cancer cells.

    abstract：BACKGROUND:Mounting evidences have showed the important role of transforming growth factor-β (TGF-β) in immunological surveillance of tumors. Some studies have also indicated human leukocyte antigen (HLA)-G-associated immune escape involving TGF-β management in gastric cancer (GC). However, the mechanism underlying it ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-015-0177-4

    authors： Guan Z,Song B,Liu F,Sun D,Wang K,Qu H

    更新日期：2015-12-02 00:00:00

  • Visfatin induces MUC8 and MUC5B expression via p38 MAPK/ROS/NF-κB in human airway epithelial cells.

    abstract：BACKGROUND:Among a variety of inflammatory mediators, visfatin is a proinflammatory adipocytokine associated with inflammatory reactions in obesity, metabolic syndrome, chronic inflammatory disease, and autoimmune disease. However, the biological role of visfatin in secretion of major mucins in human airway epithelial ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-21-49

    authors： Song SY,Jung EC,Bae CH,Choi YS,Kim YD

    更新日期：2014-05-20 00:00:00

  • Standardized, systemic phenotypic analysis reveals kidney dysfunction as main alteration of Kctd1 I27N mutant mice.

    abstract：BACKGROUND:Increased levels of blood plasma urea were used as phenotypic parameter for establishing novel mouse models for kidney diseases on the genetic background of C3H inbred mice in the phenotype-driven Munich ENU mouse mutagenesis project. The phenotypically dominant mutant line HST014 was established and further...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-017-0365-5

    authors： Kumar S,Rathkolb B,Sabrautzki S,Krebs S,Kemter E,Becker L,Beckers J,Bekeredjian R,Brommage R,Calzada-Wack J,Garrett L,Hölter SM,Horsch M,Klingenspor M,Klopstock T,Moreth K,Neff F,Rozman J,Fuchs H,Gailus-Durner V,H

    更新日期：2017-08-17 00:00:00

  • Antiviral activity of an N-allyl acridone against dengue virus.

    abstract：BACKGROUND:Dengue virus (DENV), a member of the family Flaviviridae, is at present the most widespread causative agent of a human viral disease transmitted by mosquitoes. Despite the increasing incidence of this pathogen, there are no antiviral drugs or vaccines currently available for treatment or prevention. In a pre...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-015-0134-2

    authors： Mazzucco MB,Talarico LB,Vatansever S,Carro AC,Fascio ML,D'Accorso NB,García CC,Damonte EB

    更新日期：2015-04-17 00:00:00

  • Hepatitis B virus X protein (HBx) enhances centrosomal P4.1-associated protein (CPAP) expression to promote hepatocarcinogenesis.

    abstract：BACKGROUND:Our previous report suggested that centrosomal P4.1-associated protein (CPAP) is required for Hepatitis B virus (HBV) encoded non-structure protein X (HBx)-mediated nuclear factor kappa light chain enhancer of activated B cells (NF-κB) activation. CPAP is overexpressed in HBV-associated hepatocellular carcin...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-019-0534-9

    authors： Yen CJ,Yang ST,Chen RY,Huang W,Chayama K,Lee MH,Yang SJ,Lai HS,Yen HY,Hsiao YW,Wang JM,Lin YJ,Hung LY

    更新日期：2019-06-06 00:00:00

  • Expedition to the missing link: Long noncoding RNAs in cardiovascular diseases.

    abstract：:With the advances in deep sequencing-based transcriptome profiling technology, it is now known that human genome is transcribed more pervasively than previously thought. Up to 90% of the human DNA is transcribed, and a large proportion of the human genome is transcribed as long noncoding RNAs (lncRNAs), a heterogenous...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/s12929-020-00647-w

    authors： Yeh CF,Chang YE,Lu CY,Hsuan CF,Chang WT,Yang KC

    更新日期：2020-04-02 00:00:00

  • Cascade effect of cardiac myogenesis gene expression during cardiac looping in tbx5 knockdown zebrafish embryos.

    abstract：:Zebrafish tbx5 expresses in the heart, pectoral fins and eyes of zebrafish during embryonic development. In zebrafish, injection of tbx5 morpholino antisense RNA caused changes of heart conformation, defect of heart looping, pericardium effusion, dropsy of ventral position and decreased heart rate. We suggested that c...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-008-9268-5

    authors： Lu JH,Lu JK,Choo SL,Li YC,Yeh HW,Shiue JF,Yeh VC

    更新日期：2008-11-01 00:00:00

  • A synthetic peptide sensitizes multi-drug resistant Pseudomonas aeruginosa to antibiotics for more than two hours and permeabilizes its envelope for twenty hours.

    abstract：BACKGROUND:Pseudomonas aeruginosa is a Gram-negative pathogen that frequently causes life-threatening infections in immunocompromised patients. We previously showed that subinhibitory concentrations of short synthetic peptides permeabilize P. aeruginosa and enhance the lethal action of co-administered antibiotics. MET...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-020-00678-3

    authors： Rázquin-Olazarán I,Shahrour H,Martínez-de-Tejada G

    更新日期：2020-08-06 00:00:00

  • Nitrogen-containing bisphosphonates inhibit RANKL- and M-CSF-induced osteoclast formation through the inhibition of ERK1/2 and Akt activation.

    abstract：BACKGROUND:Bisphosphonates are an important class of antiresorptive drugs used in the treatment of metabolic bone diseases. Recent studies have shown that nitrogen-containing bisphosphonates induced apoptosis in rabbit osteoclasts and prevented prenylated small GTPase. However, whether bisphosphonates inhibit osteoclas...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-21-10

    authors： Tsubaki M,Komai M,Itoh T,Imano M,Sakamoto K,Shimaoka H,Takeda T,Ogawa N,Mashimo K,Fujiwara D,Mukai J,Sakaguchi K,Satou T,Nishida S

    更新日期：2014-02-03 00:00:00

  • Effects of phorbol myristate acetate and sivelestat on the lung injury caused by fat embolism in isolated lungs.

    abstract：BACKGROUND:Fat embolism syndrome (FES) associated with acute lung injury (ALI) is a clinical condition following long bone fracture. We have reported 14 victims due to ALI with FES. Our laboratory has developed an animal model that produced fat emboli (FE). The major purpose of this study was to test whether neutrophil...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-19-3

    authors： Lin CC,Liu PH,Kao SJ,Chen HI

    更新日期：2012-01-05 00:00:00

  • PLCβ1-SHP-2 complex, PLCβ1 tyrosine dephosphorylation and SHP-2 phosphatase activity: a new part of Angiotensin II signaling?

    abstract：BACKGROUND:Angiotensin II (Ang II) signaling occurs via two major receptors which activate non-receptor tyrosin kinases that then interact with protein tyrosin-phosphatases (PTPs) to regulate cell function. SHP-2 is one such important PTP that also functions as an adaptor to promote downstream signaling pathway. Its ro...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-18-38

    authors： Calò LA,Bordin L,Davis PA,Pagnin E,Dal Maso L,Rossi GP,Pessina AC,Clari G

    更新日期：2011-06-13 00:00:00

  • Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation.

    abstract：BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-019-0602-1

    authors： Chang YH,Chu TY,Ding DC

    更新日期：2020-02-08 00:00:00

  • Down-regulation of the polymeric immunoglobulin receptor in non-small cell lung carcinoma: correlation with dysregulated expression of the transcription factors USF and AP2.

    abstract：:The polymeric immunoglobulin receptor (PIGR) mediates transport of IgA and IgM antibodies across mucosal and glandular epithelia. Several studies have utilized immunohistochemistry to demonstrate that PIGR expression varies in different types of lung carcinoma, and is down-regulated during tumor progression. We have p...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-004-8185-5

    authors： Khattar NH,Lele SM,Kaetzel CS

    更新日期：2005-01-01 00:00:00

  • Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring.

    abstract：BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of mono...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-020-00694-3

    authors： García-Ricobaraza M,García-Bermúdez M,Torres-Espinola FJ,Segura Moreno MT,Bleyere MN,Díaz-Prieto LE,Nova E,Marcos A,Campoy C

    更新日期：2020-11-30 00:00:00

  • Immunotherapy for liver tumors: present status and future prospects.

    abstract：:Increasing evidence suggests that immune responses are involved in the control of cancer and that the immune system can be manipulated in different ways to recognize and attack tumors. Progress in immune-based strategies has opened new therapeutic avenues using a number of techniques destined to eliminate malignant ce...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/1423-0127-16-30

    authors： Matar P,Alaniz L,Rozados V,Aquino JB,Malvicini M,Atorrasagasti C,Gidekel M,Silva M,Scharovsky OG,Mazzolini G

    更新日期：2009-03-06 00:00:00

  • Telomerase prevents accelerated senescence in glucose-6-phosphate dehydrogenase (G6PD)-deficient human fibroblasts.

    abstract：:Fibroblasts derived from glucose-6-phosphate dehydrogenase (G6PD)-deficient patients display retarded growth and accelerated cellular senescence that is attributable to increased accumulation of oxidative DNA damage and increased sensitivity to oxidant-induced senescence, but not to accelerated telomere attrition. Her...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-16-18

    authors： Wu YH,Cheng ML,Ho HY,Chiu DT,Wang TC

    更新日期：2009-02-05 00:00:00

  • Expression of a truncated retroviral envelope gene enhances expression of normal cellular phenotypes.

    abstract：:The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a sign...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02253367

    authors： Chen H,Chung SW,Wong PM

    更新日期：2000-11-01 00:00:00

  • Organization of HIV-1 pol is critical for Pol polyprotein processing.

    abstract：:The HIV pol sequentially encodes protease (PR), reverse transcriptase (RT), and integrase (IN) from the 5'-3' direction. We explored the significance of this gene arrangement. All six possible gene dispositions were examined. In two situations where PR was removed from the leading place and no two genes were in their ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02253522

    authors： Chang YY,Yu SL,Syu WJ

    更新日期：1999-09-01 00:00:00

  • Protective effect of a sesamin derivative, 3-bis (3-methoxybenzyl) butane-1, 4-diol on ischemic and hypoxic neuronal injury.

    abstract：BACKGROUND:Stroke is one of the leading causes of neuronal death. Sesamin is known for neuroprotection by its antioxidant and anti-inflammatory properties but it lacks blood-brain barrier (BBB) activity. A panel of sesamin derivatives was screened and 3-bis (3-methoxybenzyl) butane-1,4-diol (BBD) was selected for high ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-21-15

    authors： Hou CW,Chen YL,Chuang SH,Wang JS,Jeng KC

    更新日期：2014-02-18 00:00:00

  • Mitofusion-2-mediated alleviation of insulin resistance in rats through reduction in lipid intermediate accumulation in skeletal muscle.

    abstract：BACKGROUND:Increased lipid accumulation and mitochondrial dysfunction within skeletal muscle have been shown to be strongly associated with insulin resistance. However, the role of mitofusion-2 (MFN2), a key factor in mitochondrial function and energy metabolism, in skeletal muscle lipid intermediate accumulation remai...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-20-45

    authors： Zhang X,Wang C,Song G,Gan K,Kong D,Nie Q,Ren L

    更新日期：2013-07-01 00:00:00

  • Propofol-induced vascular permeability change is related to the nitric oxide signaling pathway and occludin phosphorylation.

    abstract：:The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-007-9164-4

    authors： Chen YS,Chen KH,Liu CC,Lee CT,Yang CH,Chuang KC,Lin CR

    更新日期：2007-09-01 00:00:00

  • Age-related expressions of p63 and other keratinocyte stem cell markers in rat cornea.

    abstract：:In this study, we examined the postnatal expression patterns of p63 and other keratinocyte stem cell markers in the rat cornea in an attempt to determine the markers that best represent characteristics of corneal keratinocyte stem cells. We show that the expression of p63 in the rat cornea is unique and differs from t...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02256130

    authors： Hsueh YJ,Wang DY,Cheng CC,Chen JK

    更新日期：2004-09-01 00:00:00