Abstract:
:The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a signal peptide. Its expression in various cell types, as compared to the control, enabled the transduced cells to assume a more normal phenotype, which is defined by an increase in contact inhibition and factor dependence, as well as reduced tumorigenicity. LN11-transduced fibroblasts exhibited a higher degree of contact inhibition, assumed a more flattened morphology and were more adherent compared to the control. In v-abl transformed hematopoietic cells, expression of LN11 resulted in slower cell growth, which was due to an enhanced dependence on exogenous growth factors. Enforced expression of LN11 also resulted in a slower rate of tumor development and a reduced tumor load. Thus, modification of a retroviral genome could have a significant impact on cell growth and development. This is one example where we need to consider the safety issue carefully when constructing retrovirus vectors for gene therapy.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chen H,Chung SW,Wong PMdoi
10.1007/BF02253367keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
514-22issue
6eissn
1021-7770issn
1423-0127pii
25487journal_volume
7pub_type
杂志文章abstract:BACKGROUND:Dengue virus (DENV), a member of the family Flaviviridae, is at present the most widespread causative agent of a human viral disease transmitted by mosquitoes. Despite the increasing incidence of this pathogen, there are no antiviral drugs or vaccines currently available for treatment or prevention. In a pre...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0134-2
更新日期:2015-04-17 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV), also designated human herpesvirus 8 (HHV-8), has been linked to Kaposi's sarcoma, as well as to primary effusion lymphoma (PEL), and a subset of multicentric Castleman's disease. KSHV genomes are maintained as episomes within infected cells and the virus exhibits a bipha...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00637-y
更新日期:2020-03-07 00:00:00
abstract::The aim of this work was to investigate the effect of a short-term exposure to somatostatin (SS), its receptors (SSTR) selective agonists as well as muscarinic receptors agonists upon acetylcholine-induced release of (3)H-MPP(+ )from bovine adrenal medullary cells. Acetylcholine (ACH, 100, 500 microM) was found to inc...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9144-0
更新日期:2007-05-01 00:00:00
abstract::Ischemia and reperfusion (I/R) - induced injury has been described as one of the main factors that contribute to the observed morbidity and mortality in a variety of clinical entities, including myocardial infarction, ischemic stroke, cardiac arrest and trauma. An imbalance between oxygen demand and supply, within the...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0220-0
更新日期:2016-01-19 00:00:00
abstract::The aim of this study was to determine whether the common forms of dyslipidemia could affect either the lipid composition or insulin receptor processing (down-regulation) of erythrocytes. The study included 22 patients with type IIa hypercholesterolemia, 15 patients with type IV hypertriglyceridemia and 12 patients wi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253384
更新日期:1995-08-01 00:00:00
abstract:BACKGROUND:Bisphosphonates are an important class of antiresorptive drugs used in the treatment of metabolic bone diseases. Recent studies have shown that nitrogen-containing bisphosphonates induced apoptosis in rabbit osteoclasts and prevented prenylated small GTPase. However, whether bisphosphonates inhibit osteoclas...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-21-10
更新日期:2014-02-03 00:00:00
abstract::Our goal was to develop a system to study proteins that associate in vivo with the Moloney murine leukemia virus (M-MuLV) enhancer elements by the isolation of intact proviral chromatin. The M-MuLV long terminal repeats (LTRs) contain tandemly repeated transcriptional enhancer sequences consisting of smaller motifs th...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256602
更新日期:2001-05-01 00:00:00
abstract::The apolipoprotein (Apo) E gene is known to be polymorphic. Three common alleles determine six phenotypes which can easily be detected by restriction fragment length polymorphism. We performed apo E genotyping in myocardial infarction survivors and healthy controls for the first time in the Turkish population. DNA was...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02257924
更新日期:1995-01-01 00:00:00
abstract::Cancer has been the leading cause of death in Taiwan over the past two decades and liver cancer is the leading cause of all cancer deaths in Taiwan with a trend of increase in incidence. Therapeutic options and efficacy for liver cancer have been limited and the 5-year survival rate is less than 7% in the Unite States...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9141-3
更新日期:2007-03-01 00:00:00
abstract::Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/s11373-005-9063-5
更新日期:2006-03-01 00:00:00
abstract::Drosophila eye development is a progressive process including cell fate determination, pattern formation, and rhabdomere morphogenesis. During eye development, a dramatic change in cell shape, which involves turning and extension of the photoreceptor apical surface, occurs in the early pupal stages. It is known that a...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254441
更新日期:2004-05-01 00:00:00
abstract::Mutations at codon 215 of HIV-1 reverse transcriptase (RT) confer resistance to nucleoside analogs through RT-catalyzed ATP-dependent phosphorolysis. We showed that mutation T215Y is predominant over T215F (respectively 38.8 vs. 7.04% of 7312 sequences from a cohort of patients receiving antiretroviral therapy in Fran...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9011-4
更新日期:2005-10-01 00:00:00
abstract::Alterations in eIF3-p48/INT6 gene expression have been implicated in murine and human mammary carcinogenesis. We examined levels of INT6 protein in human tumors and determined that breast and colon tumors clustered into distinct groups based on levels of INT6 expression and clinicopathological variables. We performed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9149-3
更新日期:2007-05-01 00:00:00
abstract::The relationship between diet and colorectal cancer has been previously demonstrated and supported with strong epidemiological evidence. The role of genetic polymorphisms has, however, been less well elaborated upon. We conducted a hospital-based case-control study including 727 cases and 736 healthy controls to evalu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9139-x
更新日期:2007-03-01 00:00:00
abstract::The ARFP/F protein is synthesized from the +1 reading frame of the hepatitis C virus (HCV) core protein gene. The function of this protein remains unknown. To study the function of the HCV ARFP/F protein, we have conducted the yeast two-hybrid screening experiment to identify cellular proteins that may interact with t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-008-9248-9
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND:Our previous report suggested that centrosomal P4.1-associated protein (CPAP) is required for Hepatitis B virus (HBV) encoded non-structure protein X (HBx)-mediated nuclear factor kappa light chain enhancer of activated B cells (NF-κB) activation. CPAP is overexpressed in HBV-associated hepatocellular carcin...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0534-9
更新日期:2019-06-06 00:00:00
abstract:BACKGROUND:Autophagy has been shown recently to play an important role in the intracellular survival of several pathogenic bacteria. In this study, we investigated the effect of a novel small-molecule autophagy-inducing agent, AR-12, on the survival of Francisella tularensis, the causative bacterium of tularemia in hum...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-110
更新日期:2009-12-09 00:00:00
abstract:BACKGROUND:Calcium oxalate (CaOx) is the major constituent of about 75% of all urinary stone and the secondary hyperoxaluria is a primary risk factor. Current treatment options for the patients with hyperoxaluria and CaOx stone diseases are limited. Oxalate degrading bacteria might have beneficial effects on urinary ox...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-014-0086-y
更新日期:2014-08-30 00:00:00
abstract::Diazoxide is a selective mitochondria ATP-sensitive potassium (K(ATP)) channel opener, which has been reported to preserve the microvascular integrity of ischemia-reperfusion (I/R)-injured tissues. Our study aimed to assess diazoxide's effects on I/R-injured cremaster muscles and to further elucidate its underlying me...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-3730-4
更新日期:2005-01-01 00:00:00
abstract::The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-004-8166-8
更新日期:2005-01-01 00:00:00
abstract::Induction of tumoricidal activity is one of the major functions of activated macrophages. Our previous study demonstrated that P388D1 murine macrophage-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent stu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255919
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Ionizing radiation (IR) activate the early growth response-1 (Egr-1) promoter by production of radical oxygen intermediates (ROIs). Egr-EF, an expression vector pCIneo containing Egr-1 promoter cloned upstream of the cDNA for Flt3 ligand, was used to treat hematopoietic damage. 5-fluorouracil, a commonly use...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-85
更新日期:2009-09-21 00:00:00
abstract:BACKGROUND:Poly(ADP-ribose) is a NAD+-requiring, DNA-repairing, enzyme playing a central role in pancreatic beta-cell death and in the development of endothelial dysfunction in humans and experimental animals. PARP activation is also relevant to the development of complications of diabetes. Hence, agents capable of inh...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S16
更新日期:2010-08-24 00:00:00
abstract:BACKGROUND:Iron homeostasis is chiefly regulated by hepcidin whose expression is tightly controlled by inflammation, iron stores, and hypoxia. Hemojuvelin (HJV) is a bone morphogenetic protein co-receptor that has been identified as a main upstream regulator of hepcidin expression; HJV mutations are associated with a s...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-83
更新日期:2012-09-21 00:00:00
abstract::In many solid tumor types, tumor-associated macrophages (TAMs) are important components of the tumor microenvironment (TME). Moreover, TAMs infiltration is strongly associated with poor survival in solid tumor patients. In this review, we describe the origins of TAMs and their polarization state dictated by the TME. W...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0568-z
更新日期:2019-10-20 00:00:00
abstract::Agents which block T cell K(+) currents can prohibit both proliferative and effector cell functions in T cells activated by mitogens or phorbol esters. This study examined the effects of some of these blocking agents on the immune responsiveness of guinea pig myelin basic protein (GPMBP)-reactive Lewis rat T lymphocyt...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255646
更新日期:1997-07-01 00:00:00
abstract:BACKGROUND:Wedelolactone (WEL), a major coumestan ingredient in Wedelia chinensis, has been used to treat septic shock, hepatitis and venom poisoning in traditional Chinese medicines. The objective of the study was to elucidate the anti-inflammatory effects and mechanism of WEL with a cellular model of lipopolysacchari...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-20-84
更新日期:2013-10-31 00:00:00
abstract:BACKGROUND:Angiotensin II (Ang II) signaling occurs via two major receptors which activate non-receptor tyrosin kinases that then interact with protein tyrosin-phosphatases (PTPs) to regulate cell function. SHP-2 is one such important PTP that also functions as an adaptor to promote downstream signaling pathway. Its ro...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-18-38
更新日期:2011-06-13 00:00:00
abstract::Chronic inflammation may lead to endothelial dysfunction, which manifests as an impaired coronary reactivity. Impairment in coronary flow reserve (CFR), preceding the clinical symptoms of coronary artery disease, can be measured noninvasively by positron emission tomography. Myeloperoxidase (MPO) is an oxidative enzym...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256549
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:The association between ischemic stroke and 2 single nucleotide polymorphisms (SNPs) on chromosome 12p13, rs12425791 and rs11833579 appears inconsistent across different samples. These SNPs are close to the ninjurin2 gene which may alter the risk of stroke by affecting brain response to ischemic injury. The ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,多中心研究
doi:10.1186/1423-0127-19-1
更新日期:2012-01-03 00:00:00