Abstract：
:Cancer has been the leading cause of death in Taiwan over the past two decades and liver cancer is the leading cause of all cancer deaths in Taiwan with a trend of increase in incidence. Therapeutic options and efficacy for liver cancer have been limited and the 5-year survival rate is less than 7% in the Unite States. The study was conducted to establish a histoculture system of human hepatocellular carcinomas (HCC) for biological and pharmacological studies and to determine the efficacy of anticancer drugs with the established HCC histocultures. Patient HCC tissues freshly obtained after surgeries were prepared and histocultured. The histocultured HCC were treated with doxorubicin and paclitaxel of various concentrations for 96-h. Upon drug treatments, the activity of tumor cell proliferation and extent of cell death induction were measured and changes of the alpha-fetoprotein levels in the culture medium were determined. We demonstrated that human HCC can be successfully cultured in a 3-dimensional histoculture system and used for pharmacological studies. Doxorubicin and paclitaxel showed concentration-dependent activities in anti-proliferation and cell death induction against the human HCC. Inhibitory effects of both drugs on alpha-fetoprotein production of the cultured HCC were in agreement with their anti-proliferative effects. Exposure time-dependent antitumoral effects of paclitaxel treatments at 3-, 24-, and 96-h against the histocultured HCC PLC/PRF/5 xenograft tumors were also observed. In conclusion, we have demonstrated a histoculture system for patient HCC and it can be utilized in selection of active drugs prior to treatments in patients and in evaluation of new agents against HCC, for which therapeutic agents are in desperate needs worldwide.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chuu JJ,Liu JM,Tsou MH,Huang CL,Chen CP,Wang HS,Chen CTdoi
10.1007/s11373-006-9141-3subject
Has Abstractpub_date
2007-03-01 00:00:00pages
233-44issue
2eissn
1021-7770issn
1423-0127journal_volume
14pub_type
杂志文章abstract：:Upon cell adhesion to extracellular matrix proteins, focal adhesion kinase (FAK) rapidly undergoes autophosphorylation on its Tyr-397 which consequently serves as a binding site for the Src homology 2 domains of the Src family protein kinases and several other intracellular signaling molecules. In this study, we have ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-7212-5
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journal_title：Journal of biomedical science
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0467-8
更新日期：2018-08-27 00:00:00
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journal_title：Journal of biomedical science
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doi：10.1186/s12929-015-0165-8
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doi：10.1186/1423-0127-18-26
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253367
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journal_title：Journal of biomedical science
pub_type： 杂志文章
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更新日期：2002-01-01 00:00:00
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journal_title：Journal of biomedical science
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doi：10.1186/s12929-015-0130-6
更新日期：2015-03-26 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0425-5
更新日期：2018-03-28 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02254368
更新日期：2004-11-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00636-z
更新日期：2020-04-07 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02255975
更新日期：2001-01-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256440
更新日期：1999-03-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0134-2
更新日期：2015-04-17 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-014-0086-y
更新日期：2014-08-30 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0246-3
更新日期：2016-03-17 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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journal_title：Journal of biomedical science
pub_type： 杂志文章
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journal_title：Journal of biomedical science
pub_type： 杂志文章
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更新日期：2010-06-24 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9204-0
更新日期：2008-01-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-S1-S25
更新日期：2010-08-24 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1159/000073523
更新日期：2003-01-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-S1-S16
更新日期：2010-08-24 00:00:00
abstract：BACKGROUND:Epigallocatechin-3-gallate (EGCg) with its potent anti-oxidative capabilities is known for its beneficial effects ameliorating oxidative injury to cardiac cells. Although studies have provided convincing evidence to support the cardioprotective effects of EGCg, it remains unclear whether EGCg affect trans-me...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-20-86
更新日期：2013-11-19 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-92
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253384
更新日期：1995-08-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9147-5
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0602-1
更新日期：2020-02-08 00:00:00
