Abstract：
BACKGROUND:Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine and elevated in the regions of tissue injury and inflammatory diseases. The deleterious effects of TNF-α on fibroblasts may aggravate heart inflammation mediated through the up-regulation of adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1). However, the mechanisms underlying TNF-α-induced VCAM-1 expression in cardiac fibroblasts remain unknown. This study aimed to investigate the roles of TNF-α in VCAM-1 expression and its effects on human cardiac fibroblasts (HCFs). RESULTS:The primary culture HCFs were used in this study. The results obtained with Western blotting, real time-quantitative PCR, and promoter activity analyses showed that TNF-α-induced VCAM-1 expression was mediated through TNF receptor (TNFR) 1-dependent gene up-regulation. Activation of TNFR1 by TNF-α transactivated c-Src-dependent EGF receptor (EGFR) linking to PI3K/Akt cascade, and then led to transcriptional activity of NF-κB. Moreover, the results of promoter reporter assay demonstrated that the phosphorylated p65 NF-κB turned on VCAM-1 gene expression. Subsequently, up-regulation of VCAM-1 promoted monocytes adhesion to HCFs challenged with TNF-α determined by cell adhesion assay. CONCLUSIONS:Taken together, these results indicate that in HCFs, activation of NF-κB by c-Src-mediated transactivation of EGFR/PI3K/Akt cascade is required for TNF-α-induced VCAM-1 expression. Finally, increased VCAM-1 enhances monocytes adhering to HCFs challenged with TNF-α. Understanding the mechanisms of VCAM-1 up-regulated by TNF-α on HCFs may provide rationally therapeutic interventions for heart injury or inflammatory diseases.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Lin CC,Pan CS,Wang CY,Liu SW,Hsiao LD,Yang CMdoi
10.1186/s12929-015-0165-8subject
Has Abstractpub_date
2015-07-15 00:00:00pages
53eissn
1021-7770issn
1423-0127pii
10.1186/s12929-015-0165-8journal_volume
22pub_type
杂志文章abstract：:The ability of cells of the human monocyte/macrophage lineage to host HIV-1 replication while resisting cell death is believed to significantly contribute to their ability to serve as a reservoir for viral replication in the host. Although macrophages are generally resistant to apoptosis, interruption of anti-apoptoti...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256024
更新日期：2002-03-01 00:00:00
abstract：:The sigma-1 receptor (Sig-1R) is a chaperone that resides mainly at the mitochondrion-associated endoplasmic reticulum (ER) membrane (called the MAMs) and acts as a dynamic pluripotent modulator in living systems. At the MAM, the Sig-1R is known to play a role in regulating the Ca2+ signaling between ER and mitochondr...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0380-6
更新日期：2017-09-16 00:00:00
abstract：:Protein kinases play pivotal roles in the development of the nervous system. They are involved in almost every stage of neuronal development, from initial proliferation and differentiation of progenitor cells to pathfinding of neurites and formation of synapses. Activation of protein kinases is also critical for neuro...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02258364
更新日期：1998-01-01 00:00:00
abstract：:Obesity is one of the most invaliding and preventable diseases in the United States. Growing evidence suggests that there are sex differences in obesity in human and experimental animals. However, the specific mechanisms of this disease are unknown. Consequently, there is any particular treatment according to the sex/...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0488-3
更新日期：2018-12-03 00:00:00
abstract：:The HIV-1 Tat protein is an RNA-binding transcriptional transactivator. Recent findings suggest that Tat associates with a cellular kinase that phosphorylates the carboxyl-terminal domain of the largest subunit of RNA polymerase II. Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcrip...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02253352
更新日期：1998-01-01 00:00:00
abstract：:Metabolic diseases such as obesity, type 2 diabetes (T2D) and insulin resistance have attracted great attention from biomedical researchers and clinicians because of the astonishing increase in its prevalence. Decrease in the capacity of oxidative metabolism and mitochondrial dysfunction are a major contributor to the...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0375-3
更新日期：2017-09-07 00:00:00
abstract：:Stem cell activity is subject to non-cell-autonomous regulation from the local microenvironment, or niche. In adaption to varying physiological conditions and the ever-changing external environment, the stem cell niche has evolved with multifunctionality that enables stem cells to detect these changes and to communica...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-0624-8
更新日期：2020-03-14 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections....
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0472-y
更新日期：2018-09-18 00:00:00
abstract：BACKGROUND:Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. METHODS:Two normal, LB and HSC93, and two transformed, Ju...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-63
更新日期：2010-07-28 00:00:00
abstract：BACKGROUND:The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus, it is of great interest to look for an...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-18-42
更新日期：2011-06-17 00:00:00
abstract：BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of mono...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00694-3
更新日期：2020-11-30 00:00:00
abstract：:The ARFP/F protein is synthesized from the +1 reading frame of the hepatitis C virus (HCV) core protein gene. The function of this protein remains unknown. To study the function of the HCV ARFP/F protein, we have conducted the yeast two-hybrid screening experiment to identify cellular proteins that may interact with t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9248-9
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Autophagy has been shown recently to play an important role in the intracellular survival of several pathogenic bacteria. In this study, we investigated the effect of a novel small-molecule autophagy-inducing agent, AR-12, on the survival of Francisella tularensis, the causative bacterium of tularemia in hum...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-110
更新日期：2009-12-09 00:00:00
abstract：BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0602-1
更新日期：2020-02-08 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via the original article. ...
journal_title：Journal of biomedical science
pub_type： 已发布勘误
doi：10.1186/s12929-020-00686-3
更新日期：2020-09-21 00:00:00
abstract：BACKGROUND:Activating transcription factor 6 (ATF6) is an endoplasmic reticulum (ER)-localised protein and member of the leucine zipper family of transcription factors. Best known for its role in transducing signals linked to stress to the endoplasmic reticulum, the 50 kDa activated form of ATF6 is now emerging as a ma...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0453-1
更新日期：2018-05-25 00:00:00
abstract：:The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransp...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-100
更新日期：2009-11-16 00:00:00
abstract：:Although hypercholesterolemia is a well-known risk factor for atherosclerosis, little is known about the effect of hypercholesterolemia on cardiac contractile function. The objective of this study was to examine the effect of hypercholesterolemia on myocardial contractility. Fifteen New Zealand white rabbits were fed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02254368
更新日期：2004-11-01 00:00:00
abstract：:Two Chinese hamster ovary (CHO) cell lines stably transfected with human insulin receptor cDNA, CHO-wt and CHO-mut, which express an equivalent number of normal and kinase-defective human insulin receptors, respectively, were used to assess the roles of insulin receptor tyrosine kinase activity in insulin-regulated ge...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02258333
更新日期：1994-12-01 00:00:00
abstract：:Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000079667
更新日期：2004-09-01 00:00:00
abstract：:Upon cell adhesion to extracellular matrix proteins, focal adhesion kinase (FAK) rapidly undergoes autophosphorylation on its Tyr-397 which consequently serves as a binding site for the Src homology 2 domains of the Src family protein kinases and several other intracellular signaling molecules. In this study, we have ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-7212-5
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:The probability of local tumor control after radiotherapy (RT) remains still miserably poor in pediatric rhabdomyosarcoma (RMS). Thus, understanding the molecular mechanisms responsible of tumor relapse is essential to identify personalized RT-based strategies. Contrary to what has been done so far, a correc...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00683-6
更新日期：2020-08-27 00:00:00
abstract：:The isoprostanes (IsoPs) are a unique series of prostaglandin-like compounds formed in vivo from the free radical-catalyzed peroxidation of arachidonic acid. This review summarizes our current knowledge regarding these compounds. Novel aspects of the biochemistry and bioactivity of IsoPs are detailed and methods by wh...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02253564
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Helicobacter pylori bacteria colonize human gastric mucosa, cause chronic inflammation, peptic ulcers and gastric cancer. Colonization is mediated by H. pylori adhesins, which preferentially bind mucin 5 (MUC5AC) and Lewis (Le) determinants. The aim of this study was to evaluate the influence of H. pylori an...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0515-z
更新日期：2019-03-06 00:00:00
abstract：:Liver cancer is the predominant cause of cancer mortality in males of Southern China and Taiwan. The current therapy is not satisfactory, and more effective treatments are needed. In the search for new therapies for liver tumor, we found that Concanavalin A (Con A), a lectin from Jack bean seeds, can have a potent ant...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/1423-0127-16-10
更新日期：2009-01-19 00:00:00
abstract：BACKGROUND:ESBL-producing bacteria are a clinical problem in the management of diseases caused by these pathogens. Worldwide, systemic infections with BL enzymes are evolving by mutations from classical bla genes in an intensified manner and they continue to be transferred across species. RESULTS:E.cloacae BF1417 isol...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0131-5
更新日期：2015-04-11 00:00:00
abstract：BACKGROUND:Next-generation sequencing (NGS) is a powerful and high-throughput method for the detection of viral mutations. This article provides a brief overview about optimization of NGS analysis for hepatocellular carcinoma (HCC)-associated hepatitis B virus (HBV) mutations, and hepatocarcinogenesis of relevant mutat...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0442-4
更新日期：2018-06-02 00:00:00
abstract：:Influence of adjuvants i.e., alpha-tocopherol (25 mg/kg, p.o.) and piperine (10 mg/kg, p.o.) on therapeutic potential of chelator tiferron (300 mg/kg, i.p.) was evaluated to encounter toxicogenic events of beryllium exposure. Albino rats were exposed to beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9147-5
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:Bisphosphonates are an important class of antiresorptive drugs used in the treatment of metabolic bone diseases. Recent studies have shown that nitrogen-containing bisphosphonates induced apoptosis in rabbit osteoclasts and prevented prenylated small GTPase. However, whether bisphosphonates inhibit osteoclas...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-10
更新日期：2014-02-03 00:00:00
abstract：BACKGROUND:Developing brain is a major target for alcohol's actions and neurological/functional abnormalities include microencephaly, reduced frontal cortex, mental retardation and attention-deficits. Previous studies have shown that ethanol altered the lateral ventricular neuroepithelial cell proliferation. However, t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0225-8
更新日期：2016-01-19 00:00:00
