Abstract：
:Protein kinases play pivotal roles in the development of the nervous system. They are involved in almost every stage of neuronal development, from initial proliferation and differentiation of progenitor cells to pathfinding of neurites and formation of synapses. Activation of protein kinases is also critical for neuronal cell survival. To gain further insights into kinases in neural development, we studied the expression patterns of protein kinases in three cultured primary neurons by degenerate primer-based reverse transcription-polymerase chain reaction (PCR) and DNA sequencing, taking advantage of all known kinases containing a conserved catalytic domain. Our data demonstrated that the expression patterns of kinases in various cultured neurons are not only different from those of non-neural tissues, but also distinct among neurons derived from discrete origins. For example, FGF receptor 1 is predominantly expressed in hippocampal neurons. As this approach may be biased during PCR and cloning steps, an RNase protection assay was employed to verify the expression levels of six kinases in cultured neurons. Results from the RNase protection assay did generally confirm those obtained by the PCR-based method. However, quantitative nature of the latter was dependent on numbers of clones analyzed, and discrepancy of expression levels of kinases detected by the two methods was sometimes observed.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Lin SD,Fann MJdoi
10.1007/BF02258364subject
Has Abstractpub_date
1998-01-01 00:00:00pages
111-9issue
2eissn
1021-7770issn
1423-0127pii
jbs05111journal_volume
5pub_type
杂志文章abstract：BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of mono...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00694-3
更新日期：2020-11-30 00:00:00
abstract：:Metabolic diseases such as obesity, type 2 diabetes (T2D) and insulin resistance have attracted great attention from biomedical researchers and clinicians because of the astonishing increase in its prevalence. Decrease in the capacity of oxidative metabolism and mitochondrial dysfunction are a major contributor to the...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0375-3
更新日期：2017-09-07 00:00:00
abstract：BACKGROUND:Lead (Pb2+) is an environmental neurotoxicant that disrupts neurodevelopment, communication, and organization through competition with Ca2+ signaling. How perinatal Pb2+ exposure affects Ca2+-related gene regulation remains unclear. However, Ca2+ activates the L-Type voltage sensitive calcium channel β-3 sub...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0450-4
更新日期：2018-05-24 00:00:00
abstract：:Maternal exposure to dietary factors during pregnancy can influence embryonic development and may modulate the phenotype of offspring through epigenetic programming. Folate is critical for nucleotide synthesis, and preconceptional intake of dietary folic acid (FA) is credited with reduced incidences of neural tube def...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-014-0077-z
更新日期：2014-08-19 00:00:00
abstract：BACKGROUND:The diastolic oscillatory after-potential Vos and pre-potential ThVos play an essential role in the pacemaker mechanism of sino-atrial node (SAN). The aim of this study was to investigate whether these oscillatory potentials are also involved in adrenergic control of SAN discharge. METHODS:Vos and ThVos wer...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-101
更新日期：2009-11-18 00:00:00
abstract：:Due to high prevalence and mortality and the lack of effective therapies, prostate cancer is one of the most crucial health problems in men. Drug resistance aggravates the situation, not only in human prostate cancer but also in other cancers. In this study, we report for the first time that cardiac glycosides (e.g. o...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256539
更新日期：2002-09-01 00:00:00
abstract：:A considerable number of animal studies on the effects of dopaminergic agents on alcohol intake behavior have been performed. Acute alcohol administration in rats induces dopamine release in the caudate nucleus and in the nucleus accumbens, an effect related among others to reinforcement. It has been repeatedly sugges...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02255975
更新日期：2001-01-01 00:00:00
abstract：:Reactive oxygen species (ROS) have been long considered simply as harmful by-products of metabolism, which damage cellular proteins, lipids, and nucleic acids. ROS are also known as a weapon of phagocytes, employed against pathogens invading the host. However, during the last decade, an understanding has emerged that ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-015-0194-3
更新日期：2015-10-15 00:00:00
abstract：:Acetylcholine (ACh), the first neurotransmitter to be identified, regulate the activities of central and peripheral functions through interactions with muscarinic receptors. Changes in muscarinic acetylcholine receptor (mAChR) have been implicated in the pathophysiology of many major diseases of the central nervous sy...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-38
更新日期：2009-04-03 00:00:00
abstract：:Ubiquitination modulates a large repertoire of cellular functions and thus, dysregulation of the ubiquitin system results in multiple human diseases, including cancer. Ubiquitination requires an E3 ligase, which is responsible for substrate recognition and conferring specificity to ubiquitination. HUWE1 is a multiface...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0470-0
更新日期：2018-09-04 00:00:00
abstract：:The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-004-8166-8
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Stroke is one of the leading causes of neuronal death. Sesamin is known for neuroprotection by its antioxidant and anti-inflammatory properties but it lacks blood-brain barrier (BBB) activity. A panel of sesamin derivatives was screened and 3-bis (3-methoxybenzyl) butane-1,4-diol (BBD) was selected for high ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-15
更新日期：2014-02-18 00:00:00
abstract：BACKGROUND:Next-generation sequencing (NGS) is a powerful and high-throughput method for the detection of viral mutations. This article provides a brief overview about optimization of NGS analysis for hepatocellular carcinoma (HCC)-associated hepatitis B virus (HBV) mutations, and hepatocarcinogenesis of relevant mutat...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0442-4
更新日期：2018-06-02 00:00:00
abstract：BACKGROUND:Parkinson's disease is the second most common neurodegenerative disorders after Alzheimer's disease. The main cause of the disease is the massive degeneration of dopaminergic neurons in the substantia nigra. Neuronal apoptosis and neuroinflammation are thought to be the key contributors to the neuronal degen...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0146-y
更新日期：2015-05-28 00:00:00
abstract：:Stem cell activity is subject to non-cell-autonomous regulation from the local microenvironment, or niche. In adaption to varying physiological conditions and the ever-changing external environment, the stem cell niche has evolved with multifunctionality that enables stem cells to detect these changes and to communica...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-0624-8
更新日期：2020-03-14 00:00:00
abstract：BACKGROUND:Ionizing radiation (IR) activate the early growth response-1 (Egr-1) promoter by production of radical oxygen intermediates (ROIs). Egr-EF, an expression vector pCIneo containing Egr-1 promoter cloned upstream of the cDNA for Flt3 ligand, was used to treat hematopoietic damage. 5-fluorouracil, a commonly use...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-85
更新日期：2009-09-21 00:00:00
abstract：:The ability of cells of the human monocyte/macrophage lineage to host HIV-1 replication while resisting cell death is believed to significantly contribute to their ability to serve as a reservoir for viral replication in the host. Although macrophages are generally resistant to apoptosis, interruption of anti-apoptoti...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256024
更新日期：2002-03-01 00:00:00
abstract：:Accumulative evidence has supported the role of nitric oxide (NO) in a variety of normal physiological functions as well as many pathological conditions. In this study, we examined the possible diabetogenicity of NO by measuring the expression of the insulin receptor substrate (IRS)-1 in rat hepatocytes and skeletal m...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-006-9073-y
更新日期：2006-07-01 00:00:00
abstract：:Two Chinese hamster ovary (CHO) cell lines stably transfected with human insulin receptor cDNA, CHO-wt and CHO-mut, which express an equivalent number of normal and kinase-defective human insulin receptors, respectively, were used to assess the roles of insulin receptor tyrosine kinase activity in insulin-regulated ge...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02258333
更新日期：1994-12-01 00:00:00
abstract：:Phospholipase D (PLD) is a phosphodiesterase that catalyses hydrolysis of phosphatidylcholine to produce phosphatidic acid and choline. In the presence of ethanol, PLD also catalyses the formation of phosphatidylethanol, which is a unique characteristic of this enzyme. Muscarinic receptor-induced changes in the activi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256440
更新日期：1999-03-01 00:00:00
abstract：:The sigma-1 receptor (Sig-1R) is a chaperone that resides mainly at the mitochondrion-associated endoplasmic reticulum (ER) membrane (called the MAMs) and acts as a dynamic pluripotent modulator in living systems. At the MAM, the Sig-1R is known to play a role in regulating the Ca2+ signaling between ER and mitochondr...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0380-6
更新日期：2017-09-16 00:00:00
abstract：:Developmentally regulated endothelial cell locus-1 (Del1) is an embryonic angiogenic factor expressed in early embryonic endothelial cells, but recently has been found to be expressed in some forms of cancers including colon and breast cancers, and melanoma, and human cancer cell lines. Overexpression of Del1 accelera...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-33
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Type I Bartter syndrome is a recessive human nephropathy caused by loss-of-function mutations in the SLC12A1 gene coding for the Na+-K+-2Cl- cotransporter NKCC2. We recently established the mutant mouse line Slc12a1I299F exhibiting kidney defects highly similar to the late-onset manifestation of this heredit...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-014-0068-0
更新日期：2014-08-02 00:00:00
abstract：BACKGROUND:Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. METHODS AND RESULTS:MSCs were treated with...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9207-x
更新日期：2008-01-01 00:00:00
abstract：:After streptococcal pyrogenic exotoxin B (SPE B) induces apoptosis, its fate is unknown. Using confocal time-course microscopy at 37 degrees C, we detected green fluorescence 20 min after adding FITC-SPE B. Orange fluorescence, an indication of co-localization of SPE B with lysosomes which were labeled with a red fluo...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9154-6
更新日期：2007-05-01 00:00:00
abstract：:While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causa...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9204-0
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Oxidized LDL (oxLDL) is involved in the development of atherosclerotic heart disease through a mechanism that is not fully understood. In this study, we examined the role of malondialdehyde (MDA), an important oxidative stress epitope of oxLDL, in mediating coronary endothelial cytotoxicity. RESULTS:Human c...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-11
更新日期：2014-02-03 00:00:00
abstract：:The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256432
更新日期：2003-07-01 00:00:00
abstract：:This study investigated the involvement of neutrophil infiltration, disturbances in nitric oxide (NO) generation and oxidative stress in indomethacin-induced gastric ulcer, and the possible gastroprotective potentials of leptin, known for its angiogenic effect. Male Wistar albino rats (180-220 g) were allocated into a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9227-6
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:Acid-sensing ion channels (ASICs) are a group of amiloride-sensitive ligand-gated ion channels belonging to the family of degenerin/epithelial sodium channels. ASICs are predominantly expressed in both the peripheral and central nervous system and have been characterized as potent proton sensors to detect ex...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0448-y
更新日期：2018-05-24 00:00:00
