Abstract：
:This study investigated the involvement of neutrophil infiltration, disturbances in nitric oxide (NO) generation and oxidative stress in indomethacin-induced gastric ulcer, and the possible gastroprotective potentials of leptin, known for its angiogenic effect. Male Wistar albino rats (180-220 g) were allocated into a normal control group, ulcer control group (received a single dose of indomethacin 40 mg/kg p.o.) and an ulcer group pretreated with leptin (10 microg/kg i.p. 30 min before ulcer induction). The animals were killed 6 h after indomethacin administration and their gastric juice, serum and mucosal tissue were used for gastric injury evaluation. Indomethacin produced multiple lesions in glandular mucosa, evidenced by marked increase in gastric ulcer index (GUI) accompanied by significant increases in gastric juice acidity, tissue myeloperoxidase (MPO) activity, serum NO and tissue conjugated diene (CD), and marked decreases in tissue NO and glutathione (GSH) as well as glutathione reductase (GR) and superoxide dismutase (SOD) activities, while gastric juice mucin and tissue glutathione peroxidase (GPx) were not affected. Leptin exerted significant gastroprotection as evidenced by significantly decreased GUI and attenuated neutrophil infiltration. Leptin significantly increased mucin and tissue NO, restored GR and SOD activities and up-regulated GPx activity. It failed to affect acidity, serum NO, GSH and CD. These results suggest that leptin confers significant gastroprotection against indomethacin-induced injury through interfering with neutrophil infiltration, NO production and oxidative stress.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Motawi TK,Abd Elgawad HM,Shahin NNdoi
10.1007/s11373-007-9227-6subject
Has Abstractpub_date
2008-05-01 00:00:00pages
405-12issue
3eissn
1021-7770issn
1423-0127journal_volume
15pub_type
杂志文章abstract：BACKGROUND:The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus, it is of great interest to look for an...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-18-42
更新日期：2011-06-17 00:00:00
abstract：:The isoprostanes (IsoPs) are a unique series of prostaglandin-like compounds formed in vivo from the free radical-catalyzed peroxidation of arachidonic acid. This review summarizes our current knowledge regarding these compounds. Novel aspects of the biochemistry and bioactivity of IsoPs are detailed and methods by wh...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/BF02253564
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Prenatal alcohol exposure may cause cardiac development defects, however, the underlying mechanisms are not yet clear. In the present study we have investigated the roles of histone modification by curcumin on alcohol induced fetal cardiac abnormalities during the development. METHODS AND RESULTS:Q-PCR and ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0310-z
更新日期：2017-01-05 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections....
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0472-y
更新日期：2018-09-18 00:00:00
abstract：BACKGROUND:Type I Bartter syndrome is a recessive human nephropathy caused by loss-of-function mutations in the SLC12A1 gene coding for the Na+-K+-2Cl- cotransporter NKCC2. We recently established the mutant mouse line Slc12a1I299F exhibiting kidney defects highly similar to the late-onset manifestation of this heredit...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-014-0068-0
更新日期：2014-08-02 00:00:00
abstract：BACKGROUND:Glutaminase predominates in periportal hepatocytes and it has been proposed that it determines the glutamine-derived nitrogen flow through the urea cycle. Glutamine-derived urea production should, thus, be considerably faster in periportal hepatocytes. This postulate, based on indirect observations, has not ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-1
更新日期：2010-01-07 00:00:00
abstract：BACKGROUND:Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. METHODS AND RESULTS:MSCs were treated with...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9207-x
更新日期：2008-01-01 00:00:00
abstract：:The goal of this study was to evaluate the effect of chronic Zn2+ administration (1 mg/kg/day for 1 month) in Sprague-Dawley rats (n = 11) on motility and rearing behaviors (number of events/10 min measured in motility cage), on memory (percentage of failures using a foot-shock double T maze), on the number of muscari...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1159/000067296
更新日期：2002-11-01 00:00:00
abstract：:The first- and second-generation epidermal growth factor receptor tyrosine kinase inhibitors (1/2G EGFR-TKIs) gefitinib, erlotinib, and afatinib have all been approved as standard first-line treatments for advanced EGFR mutation-positive non-small cell lung cancer. The third-generation (3G) EGFR-TKIs have been develop...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0305-9
更新日期：2016-12-03 00:00:00
abstract：BACKGROUND:Activating transcription factor 6 (ATF6) is an endoplasmic reticulum (ER)-localised protein and member of the leucine zipper family of transcription factors. Best known for its role in transducing signals linked to stress to the endoplasmic reticulum, the 50 kDa activated form of ATF6 is now emerging as a ma...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0453-1
更新日期：2018-05-25 00:00:00
abstract：BACKGROUND:Wedelolactone (WEL), a major coumestan ingredient in Wedelia chinensis, has been used to treat septic shock, hepatitis and venom poisoning in traditional Chinese medicines. The objective of the study was to elucidate the anti-inflammatory effects and mechanism of WEL with a cellular model of lipopolysacchari...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-20-84
更新日期：2013-10-31 00:00:00
abstract：:Endotoxin shock is characterized by systemic hypotension, hyporeactiveness to vasoconstrictors and acute lung edema. A nitric oxide synthase (NOS) inhibitor, NG-monomethyl-L-arginine (L-NMMA) has been shown to be effective in reversing acute lung injury. In the present study, we evaluated the effects of NOS blockade b...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256421
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Fat embolism syndrome (FES) associated with acute lung injury (ALI) is a clinical condition following long bone fracture. We have reported 14 victims due to ALI with FES. Our laboratory has developed an animal model that produced fat emboli (FE). The major purpose of this study was to test whether neutrophil...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-3
更新日期：2012-01-05 00:00:00
abstract：:We have generated peptide antisera against selected regions in HIV-1 and HIV-2 reverse transcriptase (RT) and integrase (IN) to investigate the specificity of determinants governing the immune response. Peptides representing homologous regions (>50%) in the N- and C-termini and central portions of these proteins were ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253469
更新日期：1998-01-01 00:00:00
abstract：:While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causa...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9204-0
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Zerumbone, a sesquiterpene compound isolated from subtropical ginger, Zingiber zerumbet Smith, has been documented to exert antitumoral and anti- inflammatory activities. In this study, we demonstrate that zerumbone induces apoptosis in human glioblastoma multiforme (GBM8401) cells and investigate the apopto...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-86
更新日期：2012-10-05 00:00:00
abstract：:The Tat proteins of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2), termed Tat-1 and Tat-2, respectively, are essential for efficient viral replication. Tat proteins activate viral transcription by binding to the TAR RNA stem-loop structure at the 5' end of viral transcripts. We used an in vitro selection ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255590
更新日期：1997-01-01 00:00:00
abstract：:Hypoxia is an important microenvironmental factor that induces cancer metastasis. Hypoxia/hypoxia-inducible factor-1α (HIF-1α) regulates many important steps of the metastatic processes, especially epithelial-mesenchymal transition (EMT) that is one of the crucial mechanisms to cause early stage of tumor metastasis. T...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/1423-0127-19-102
更新日期：2012-12-14 00:00:00
abstract：:The midbrain periaqueductal gray matter (PAG) is an important brain region for the coordination of mu-opioid-induced pharmacological actions. The present study was designed to determine whether newly isolated mu-opioid peptide endomorphins can activate G proteins through mu-opioid receptors in the PAG by monitoring th...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255469
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:Promoting post-stroke neurogenesis has long been proposed to be a therapeutic strategy for the enhancement of functional recovery after cerebral ischemic stroke. Despite numerous approaches have been widely reported the proliferation or differentiation of the neurogenic population therapeutic strategies by t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0521-1
更新日期：2019-04-18 00:00:00
abstract：:Ubiquitination modulates a large repertoire of cellular functions and thus, dysregulation of the ubiquitin system results in multiple human diseases, including cancer. Ubiquitination requires an E3 ligase, which is responsible for substrate recognition and conferring specificity to ubiquitination. HUWE1 is a multiface...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0470-0
更新日期：2018-09-04 00:00:00
abstract：:The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a sign...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253367
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:Autophagy has been shown recently to play an important role in the intracellular survival of several pathogenic bacteria. In this study, we investigated the effect of a novel small-molecule autophagy-inducing agent, AR-12, on the survival of Francisella tularensis, the causative bacterium of tularemia in hum...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-110
更新日期：2009-12-09 00:00:00
abstract：BACKGROUND:Cell adhesion has been shown to induce activation of certain growth factor receptors in a ligand-independent manner. However, the mechanism for such activation remains obscure. METHODS:Human epidermal carcinoma A431 cells were used as a model to examine the mechanism for adhesion-induced activation of hepat...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-18-78
更新日期：2011-10-27 00:00:00
abstract：:As a neurotropic virus, enterovirus A71 (EV-A71) emerge and remerge in the Asia-Pacific region since the 1990s, and has continuously been a threat to global public health, especially in children. Annually, EV-A71 results in hand-foot-and-mouth disease (HFMD) and occasionally causes severe neurological disease. Here we...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-019-0574-1
更新日期：2019-10-21 00:00:00
abstract：:The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9164-4
更新日期：2007-09-01 00:00:00
abstract：:Maternal exposure to dietary factors during pregnancy can influence embryonic development and may modulate the phenotype of offspring through epigenetic programming. Folate is critical for nucleotide synthesis, and preconceptional intake of dietary folic acid (FA) is credited with reduced incidences of neural tube def...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-014-0077-z
更新日期：2014-08-19 00:00:00
abstract：:Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy in terms of clinical features, underlying pathogenesis and treatment outcomes. Recent advances in genomic techniques have unraveled the molecular complexity of AML leukemogenesis, which in turn have led to refinement of risk stratification and pers...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00674-7
更新日期：2020-07-21 00:00:00
abstract：:The HIV pol sequentially encodes protease (PR), reverse transcriptase (RT), and integrase (IN) from the 5'-3' direction. We explored the significance of this gene arrangement. All six possible gene dispositions were examined. In two situations where PR was removed from the leading place and no two genes were in their ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253522
更新日期：1999-09-01 00:00:00
abstract：:Recent transcriptome analyses have revealed that noncoding RNAs (ncRNAs) are broadly expressed in mammalian cells and abundant in the CNS, with tissue and cell type-specific expression patterns. Moreover, ncRNAs have been found to intricately and dynamically regulate various signaling pathways in neurodegeneration. As...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00636-z
更新日期：2020-04-07 00:00:00
