Abstract:
:The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans blue content of the dorsal skin of the infused limb at 15, 30, 45 and 60 min after propofol injection. The total content of the tight junction proteins occludin, ZO-1 and claudin-5 under experimental conditions was also determined by western blotting. Intra-arterial injection with propofol resulted in a marked dose-dependent increase in vascular permeability of the rat hindpaw. Pretreatment with 10 mg/kg of N-nitro-L: -arginine methyl ester (L: -NAME) but not aminoguanidine significantly inhibited the change in vascular permeability after challenge with propofol. Pretreatment with L: -arginine and nitroprusside increased the propofol-induced permeability change. Intra-arterial injection of propofol significantly increased occludin phosphorylation after 15 min, which was consistent with the time profile of the vascular permeability change. L: -NAME partially reversed the change in occludin phosphorylation, whereas aminoguanidine had no effect compared with that in the controls. Our observations indicate that nitric oxide (NO) is an important mediator in the induction of vascular permeability induced by propofol. Occludin phosphorylation is a determining factor in the vascular permeability change induced by propofol. NO synthase (NOS) inhibitors might be useful in the treatment of accidental intra-arterial injection of propofol, in the reduction of any adverse effects.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chen YS,Chen KH,Liu CC,Lee CT,Yang CH,Chuang KC,Lin CRdoi
10.1007/s11373-007-9164-4subject
Has Abstractpub_date
2007-09-01 00:00:00pages
629-36issue
5eissn
1021-7770issn
1423-0127journal_volume
14pub_type
杂志文章abstract::As a neurotropic virus, enterovirus A71 (EV-A71) emerge and remerge in the Asia-Pacific region since the 1990s, and has continuously been a threat to global public health, especially in children. Annually, EV-A71 results in hand-foot-and-mouth disease (HFMD) and occasionally causes severe neurological disease. Here we...
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章,多中心研究
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更新日期:2020-08-06 00:00:00
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pub_type: 杂志文章
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