当前位置： SCI文献检索 > JOURNAL OF BIOMEDICAL SCIENCE期刊下所有文献 > Overexpression of Myostatin2 in zebrafish reduces the expression of dystrophin associated protein complex (DAPC) which leads to muscle dystrophy.

Overexpression of Myostatin2 in zebrafish reduces the expression of dystrophin associated protein complex (DAPC) which leads to muscle dystrophy.

Abstract：

:Myostatin, a member of the TGF-beta superfamily, is a potent negative regulator of skeletal muscle and growth. Previously, we reported Mstn1 from zebrafish and studied its influence on muscle development. In this study, we identified another form of Myostatin protein which is referred to as Mstn2. The size of Mstn2 cDNA is 1342 bp with 109 and 132 bp of 5' and 3'-untranslated regions (UTRs), respectively. The coding region is 1101 bp encoding 367 amino acids. The identity between zebrafish Mstn1 and 2 is 66%. The phylogenetic tree revealed that the Mstn2 is an ancestral form of Mstn1. To study the functional aspects, we overexpressed mstn2 and noticed that embryos became less active and the juveniles with bent and curved phenotypes when compared to the control. The RT-PCR and in situ hybridization showed concurrent reduction of dystrophin associated protein complex (DAPC). In cryosection and in situ hybridization, we observed the disintegration of somites, lack of transverse myoseptum and loss of muscle integrity due to the failure of muscle attachment in mstn2 overexpressed embryos. Immunohistochemistry and western blot showed that there was a reduction of dystrophin, dystroglycan and sarcoglycan at translational level in overexpressed embryos. Taken together, these results indicate the suitability of zebrafish as an excellent animal model and our data provide the first in vivo evidence of muscle attachment failure by the overexpression of mstn2 and it leads to muscle loss which results in muscle dystrophy that may contribute to Duchenne syndrome and other muscle related diseases.

journal_name

J Biomed Sci

journal_title

Journal of biomedical science

authors

Amali AA,Lin CJ,Chen YH,Wang WL,Gong HY,Rekha RD,Lu JK,Chen TT,Wu JL

doi

10.1007/s11373-008-9250-2

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

595-604

issue

5

eissn

1021-7770

issn

1423-0127

journal_volume

15

pub_type

杂志文章

相关文献

JOURNAL OF BIOMEDICAL SCIENCE文献大全
  • DHEA attenuates catecholamine secretion from bovine adrenal chromaffin cells.

    abstract：:Dehydroepiandrosterone (DHEA) is a putative anti-stress agent and stress is associated with the secretion of catecholamine from the adrenal gland, but the effects of DHEA on catecholamine secretion are not fully understood. Using bovine chromaffin cells, we found that DHEA inhibited catecholamine secretion and cytosol...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02256563

    authors： Liu PS,Wang PY

    更新日期：2004-03-01 00:00:00

  • Insulin Receptor Processing and Lipid Composition of Erythrocyte Membrane in Patients with Hyperlipidemia.

    abstract：:The aim of this study was to determine whether the common forms of dyslipidemia could affect either the lipid composition or insulin receptor processing (down-regulation) of erythrocytes. The study included 22 patients with type IIa hypercholesterolemia, 15 patients with type IV hypertriglyceridemia and 12 patients wi...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02253384

    authors： Masella R,Cantafora A,Maffì D,Volpe R,Ginnetti MG,Ricci G,Mace NL,Buxton GM,Peterson SW

    更新日期：1995-08-01 00:00:00

  • Ubiquitination by HUWE1 in tumorigenesis and beyond.

    abstract：:Ubiquitination modulates a large repertoire of cellular functions and thus, dysregulation of the ubiquitin system results in multiple human diseases, including cancer. Ubiquitination requires an E3 ligase, which is responsible for substrate recognition and conferring specificity to ubiquitination. HUWE1 is a multiface...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/s12929-018-0470-0

    authors： Kao SH,Wu HT,Wu KJ

    更新日期：2018-09-04 00:00:00

  • Gastroprotective effect of leptin in indomethacin-induced gastric injury.

    abstract：:This study investigated the involvement of neutrophil infiltration, disturbances in nitric oxide (NO) generation and oxidative stress in indomethacin-induced gastric ulcer, and the possible gastroprotective potentials of leptin, known for its angiogenic effect. Male Wistar albino rats (180-220 g) were allocated into a...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-007-9227-6

    authors： Motawi TK,Abd Elgawad HM,Shahin NN

    更新日期：2008-05-01 00:00:00

  • Update on recent preclinical and clinical studies of T790M mutant-specific irreversible epidermal growth factor receptor tyrosine kinase inhibitors.

    abstract：:The first- and second-generation epidermal growth factor receptor tyrosine kinase inhibitors (1/2G EGFR-TKIs) gefitinib, erlotinib, and afatinib have all been approved as standard first-line treatments for advanced EGFR mutation-positive non-small cell lung cancer. The third-generation (3G) EGFR-TKIs have been develop...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/s12929-016-0305-9

    authors： Liao BC,Lin CC,Lee JH,Yang JC

    更新日期：2016-12-03 00:00:00

  • The fate of SPE B after internalization and its implication in SPEB-induced apoptosis.

    abstract：:After streptococcal pyrogenic exotoxin B (SPE B) induces apoptosis, its fate is unknown. Using confocal time-course microscopy at 37 degrees C, we detected green fluorescence 20 min after adding FITC-SPE B. Orange fluorescence, an indication of co-localization of SPE B with lysosomes which were labeled with a red fluo...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-007-9154-6

    authors： Chang CW,Tsai WH,Chuang WJ,Lin YS,Wu JJ,Liu CC,Tsai PJ,Lin MT

    更新日期：2007-05-01 00:00:00

  • Fibronectin inhibitor pUR4 attenuates tumor necrosis factor α-induced endothelial hyperpermeability by modulating β1 integrin activation.

    abstract：BACKGROUND:The blood-spinal cord barrier (BSCB) is composed of a monolayer of endothelium linked with tight junctions and extracellular matrix (ECM)-rich basement membranes and is surrounded by astrocyte foot processes. Endothelial permeability is regulated by interaction between endothelial cells and ECM proteins. Fib...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-019-0529-6

    authors： Lee TH,Hsieh ST,Chiang HY

    更新日期：2019-05-16 00:00:00

  • Differential effect of the focal adhesion kinase Y397F mutant on v-Src-stimulated cell invasion and tumor growth.

    abstract：:Upon cell adhesion to extracellular matrix proteins, focal adhesion kinase (FAK) rapidly undergoes autophosphorylation on its Tyr-397 which consequently serves as a binding site for the Src homology 2 domains of the Src family protein kinases and several other intracellular signaling molecules. In this study, we have ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-005-7212-5

    authors： Chang LC,Huang CH,Cheng CH,Chen BH,Chen HC

    更新日期：2005-01-01 00:00:00

  • Engagement of ubiquitination and de-ubiquitination at rostral ventrolateral medulla in experimental brain death.

    abstract：BACKGROUND:Whereas brain death is a vitally important clinical phenomenon, our contemporary understanding on its underlying cellular mechanisms remains elusive. This study evaluated whether the ubiquitin-proteasome system (UPS) in the rostral ventrolateral medulla (RVLM), a neural substrate that our laboratory identifi...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-19-48

    authors： Wu CH,Chan JY,Chou JL,Chan SH,Chang AY

    更新日期：2012-04-30 00:00:00

  • Whole blood-derived microRNA signatures in mice exposed to lipopolysaccharides.

    abstract：BACKGROUND:Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole blood from mice injected with int...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-19-69

    authors： Hsieh CH,Rau CS,Jeng JC,Chen YC,Lu TH,Wu CJ,Wu YC,Tzeng SL,Yang JC

    更新日期：2012-07-31 00:00:00

  • Neuroprotective effect of novel cognitive enhancer noopept on AD-related cellular model involves the attenuation of apoptosis and tau hyperphosphorylation.

    abstract：BACKGROUND:Noopept (N-phenyl-acetyl-L-prolylglycine ethyl ester) was constructed as a dipeptide analog of the standard cognition enhancer, piracetam. Our previous experiments have demonstrated the cognition restoring effect of noopept in several animal models of Alzheimer disease (AD). Noopept was also shown to prevent...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-014-0074-2

    authors： Ostrovskaya RU,Vakhitova YV,Kuzmina USh,Salimgareeva MKh,Zainullina LF,Gudasheva TA,Vakhitov VA,Seredenin SB

    更新日期：2014-08-06 00:00:00

  • Long non-coding RNA Gas5 regulates proliferation and apoptosis in HCS-2/8 cells and growth plate chondrocytes by controlling FGF1 expression via miR-21 regulation.

    abstract：BACKGROUND:LncRNA Gas5 is known to be a key control element during growth, differentiation and development in mammalian species. However, the role and function of Gas5 in growth plate chondrocytes has not been determined. METHODS:The overexpression and knockdown models of Gas5 and miR-21 in cells and animals were cons...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-018-0424-6

    authors： Liu X,She Y,Wu H,Zhong D,Zhang J

    更新日期：2018-02-28 00:00:00

  • Antiviral activity of an N-allyl acridone against dengue virus.

    abstract：BACKGROUND:Dengue virus (DENV), a member of the family Flaviviridae, is at present the most widespread causative agent of a human viral disease transmitted by mosquitoes. Despite the increasing incidence of this pathogen, there are no antiviral drugs or vaccines currently available for treatment or prevention. In a pre...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-015-0134-2

    authors： Mazzucco MB,Talarico LB,Vatansever S,Carro AC,Fascio ML,D'Accorso NB,García CC,Damonte EB

    更新日期：2015-04-17 00:00:00

  • Induction of Fas and Fas-ligand expression in plasmacytoma cells by a cytotoxic factor secreted by murine macrophages.

    abstract：:Induction of tumoricidal activity is one of the major functions of activated macrophages. Our previous study demonstrated that P388D1 murine macrophage-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent stu...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02255919

    authors： Chu CY,Tseng J

    更新日期：2000-01-01 00:00:00

  • Functional Analysis of the ACTGCTGA Sequence Motif in the Human Immunodeficiency Virus Type-1 Long Terminal Repeat Promoter.

    abstract：:The ACTGCTGA sequence (CTG motif) is located immediately upstream of the NF-kappaB enhancer in the human immunodeficiency virus type-1 (HIV-1) long terminal repeat (LTR). We previously reported on the frequent duplication of this motif in HIV-1-infected individuals. In this study we further characterized the role of t...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02257981

    authors： Koken SE,van Wamel JL,Geelen JL,Berkhout B

    更新日期：1994-03-01 00:00:00

  • Lithospermic acid attenuates 1-methyl-4-phenylpyridine-induced neurotoxicity by blocking neuronal apoptotic and neuroinflammatory pathways.

    abstract：BACKGROUND:Parkinson's disease is the second most common neurodegenerative disorders after Alzheimer's disease. The main cause of the disease is the massive degeneration of dopaminergic neurons in the substantia nigra. Neuronal apoptosis and neuroinflammation are thought to be the key contributors to the neuronal degen...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-015-0146-y

    authors： Lin YL,Tsay HJ,Lai TH,Tzeng TT,Shiao YJ

    更新日期：2015-05-28 00:00:00

  • Propofol-induced vascular permeability change is related to the nitric oxide signaling pathway and occludin phosphorylation.

    abstract：:The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-007-9164-4

    authors： Chen YS,Chen KH,Liu CC,Lee CT,Yang CH,Chuang KC,Lin CR

    更新日期：2007-09-01 00:00:00

  • Supranodose vagotomy precludes reflex respiratory responses to serotonin in cats.

    abstract：:Mediation of the respiratory reflex effects of an exogenous serotonin challenge goes beyond the lung vagi and is suggested to involve the nodose ganglia. In the present experiments the effects of an intravenous serotonin challenge on breathing pattern were studied in 8 pentobarbitone-chloralose anaesthetised cats. Bol...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02256323

    authors： Kopczyńska B,Szereda-Przestaszewska M

    更新日期：2003-11-01 00:00:00

  • Decreased total nitric oxide production in patients with duchenne muscular dystrophy.

    abstract：:Plasma nitric oxide (NO) levels in Duchenne muscular dystrophy (DMD) patients were significantly lower than those observed in both healthy controls and in patients with other neuromuscular disorders. The correlation between NO level and ejection fraction was significant (r = -0.384, p = 0.0391) in the DMD group. Disru...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02256104

    authors： Kasai T,Abeyama K,Hashiguchi T,Fukunaga H,Osame M,Maruyama I

    更新日期：2004-07-01 00:00:00

  • Molecular oncology focus - is carcinogenesis a 'mitochondriopathy'?

    abstract：:Mitochondria are sub-cellular organelles that produce adenosine triphosphate (ATP) through oxidative phosphorylation (OXPHOS). As suggested over 70 years ago by Otto Warburg and recently confirmed with molecular techniques, alterations in respiratory activity and in mitochondrial DNA (mtDNA) appear to be common featur...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/1423-0127-17-31

    authors： Czarnecka AM,Czarnecki JS,Kukwa W,Cappello F,Scińska A,Kukwa A

    更新日期：2010-04-25 00:00:00

  • B cell subset alteration and the expression of tissue homing molecules in dengue infected patients.

    abstract：BACKGROUND:B cells play an essential role during dengue viral infection. While a major expansion of antibody secreting cells (ASCs) was observed, the importance of these increased frequencies of ASCs remains unclear. The alteration of B cell subsets may result from the expression of tissue specific homing molecules lea...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/s12929-018-0467-8

    authors： Pattanapanyasat K,Khowawisetsut L,Chuansumrit A,Chokephaibulkit K,Tangnararatchakit K,Apiwattanakul N,Techasaensiri C,Thitilertdecha P,Sae-Ung T,Onlamoon N

    更新日期：2018-08-27 00:00:00

  • Roles of sigma-1 receptors on mitochondrial functions relevant to neurodegenerative diseases.

    abstract：:The sigma-1 receptor (Sig-1R) is a chaperone that resides mainly at the mitochondrion-associated endoplasmic reticulum (ER) membrane (called the MAMs) and acts as a dynamic pluripotent modulator in living systems. At the MAM, the Sig-1R is known to play a role in regulating the Ca2+ signaling between ER and mitochondr...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/s12929-017-0380-6

    authors： Weng TY,Tsai SA,Su TP

    更新日期：2017-09-16 00:00:00

  • Distinct population of highly malignant cells in a head and neck squamous cell carcinoma cell line established by xenograft model.

    abstract：:The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransp...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-16-100

    authors： Chen CY,Chiou SH,Huang CY,Jan CI,Lin SC,Tsai ML,Lo JF

    更新日期：2009-11-16 00:00:00

  • Effect of beta-alanine treatment on mitochondrial taurine level and 5-taurinomethyluridine content.

    abstract：BACKGROUND:The beta-amino acid, taurine, is a nutritional requirement in some species. In these species, the depletion of intracellular stores of taurine leads to the development of severe organ dysfunction. The basis underlying these defects is poorly understood, although there is some suggestion that oxidative stress...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-17-S1-S25

    authors： Jong CJ,Ito T,Mozaffari M,Azuma J,Schaffer S

    更新日期：2010-08-24 00:00:00

  • Fish-on-a-chip: a sensitive detection microfluidic system for Alzheimer's disease.

    abstract：:Microfluidics has become an important tool in diagnosing many diseases, including neurological and genetic disorders. Alzheimer's disease (AD) is a neurodegenerative disease that irreversibly and progressively destroys memory, language ability, and thinking skills. Commonly, detection of AD is expensive and complex. F...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章,评审

    doi：10.1186/1423-0127-18-33

    authors： Devadhasan JP,Kim S,An J

    更新日期：2011-05-28 00:00:00

  • Effects of single or repeated dermal exposure to methyl parathion on behavior and blood cholinesterase activity in rats.

    abstract：:The effects of a single or repeated dermal administration of methyl parathion on motor function, learning and memory were investigated in adult female rats and correlated with blood cholinesterase activity. Exposure to a single dose of 50 mg/kg methyl parathion (75% of the dermal LD(50)) resulted in an 88% inhibition ...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02256609

    authors： Zhu H,Rockhold RW,Baker RC,Kramer RE,Ho IK

    更新日期：2001-11-01 00:00:00

  • Apolipoprotein E Genotyping in Turkish Myocardial Infarction Survivors and Healthy Controls.

    abstract：:The apolipoprotein (Apo) E gene is known to be polymorphic. Three common alleles determine six phenotypes which can easily be detected by restriction fragment length polymorphism. We performed apo E genotyping in myocardial infarction survivors and healthy controls for the first time in the Turkish population. DNA was...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/BF02257924

    authors： Hergenc G,Taga Y,Emerk K,Cirakoglu B

    更新日期：1995-01-01 00:00:00

  • Association between transforming growth factor beta1 polymorphisms and atrial fibrillation in essential hypertensive subjects.

    abstract：BACKGROUND:The association of TGF beta1 polymorphisms and atrial fibrillation (AF) in essential hypertensive (EH) subjects remains unknown. Methods EH subjects with AF (EH+AF+) and sinus rhythm (EH+AF-) were enrolled. The polymorphisms of +869 T --> C at codon 10 and + 915 G --> C at codon 25, were genotyped. The clini...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1186/1423-0127-17-23

    authors： Wang Y,Hou X,Li Y

    更新日期：2010-03-31 00:00:00

  • The role of nitric oxide in the spatial heterogeneity of basal microvascular blood flow in the rat diaphragm.

    abstract：:The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-004-8166-8

    authors： Lee CH,Chang HY,Chen CW,Hsiue TR

    更新日期：2005-01-01 00:00:00

  • Different pathways leading to activation of extracellular signal-regulated kinase and p38 MAP kinase by formyl-methionyl-leucyl-phenylalanine or platelet activating factor in human neutrophils.

    abstract：:The signaling pathways leading to extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) activation by N-formyl-Met-Leu-Phe (fMLP) or platelet activating factor (PAF) in human neutrophils were examined. Previously, we found that changes of intracellular Ca2+ ([Ca2+]i) stimulated by...

    journal_title：Journal of biomedical science

    pub_type： 杂志文章

    doi：10.1007/s11373-005-1704-1

    authors： Chen LW,Lin MW,Hsu CM

    更新日期：2005-01-01 00:00:00