Abstract:
:The signaling pathways leading to extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) activation by N-formyl-Met-Leu-Phe (fMLP) or platelet activating factor (PAF) in human neutrophils were examined. Previously, we found that changes of intracellular Ca2+ ([Ca2+]i) stimulated by PAF and fMLP were due to Ca2+ influx and internal Ca2+ release, respectively. To further determine the mechanism of MAPK activation and its relation with Ca2+ influx, blood from healthy human volunteers was taken by venous puncture. Human polymorphonuclear cells (PMNs) were isolated and incubated with protein kinase C (PKC) inhibitor Calphostin C, PKC-gamma isoform inhibitor GF109203X, phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY294002, phospholipase C (PLC) inhibitor U73122, phospholipase A2 (PLA2) inhibitor Aristolochic acid, store-operated calcium (SOC) channel inhibitor SKF96365, or extracellular calcium chelator EGTA followed by fMLP or PAF treatment. Phosphorylation of ERK p38 was determined by immunoblotting analysis. Our data indicate that neutrophil MAPK signaling pathways mediated by fMLP and PAF are different. PAF-induced ERK phosphorylation is mediated by PI3K, PKC, PLA2, PLC, and extracellular calcium, whereas fMLP-induced ERK phosphorylation does not involve the PKC-gamma isoform and extracellular calcium. PAF-induced p38 phosphorylation involves PLA2, whereas fMLP-induced p38 activation is PLC dependent.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chen LW,Lin MW,Hsu CMdoi
10.1007/s11373-005-1704-1keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
311-9issue
2eissn
1021-7770issn
1423-0127journal_volume
12pub_type
杂志文章abstract:BACKGROUND:The enzyme-prodrug system is considered a promising tool for tumor treatment when conjugated with a targeting molecule. The asparagine-glycine-arginine (NGR) motif is a developing and interesting targeting peptide that could specifically bind to aminopeptidase N (APN), which is an NGR receptor expressed on t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-016-0227-6
更新日期:2016-01-22 00:00:00
abstract:BACKGROUND:Our previous report suggested that centrosomal P4.1-associated protein (CPAP) is required for Hepatitis B virus (HBV) encoded non-structure protein X (HBx)-mediated nuclear factor kappa light chain enhancer of activated B cells (NF-κB) activation. CPAP is overexpressed in HBV-associated hepatocellular carcin...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0534-9
更新日期:2019-06-06 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:2019-01-15 00:00:00
abstract::Fibroblasts derived from glucose-6-phosphate dehydrogenase (G6PD)-deficient patients display retarded growth and accelerated cellular senescence that is attributable to increased accumulation of oxidative DNA damage and increased sensitivity to oxidant-induced senescence, but not to accelerated telomere attrition. Her...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-18
更新日期:2009-02-05 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0198-z
更新日期:2015-12-08 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256452
更新日期:2003-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-017-0367-3
更新日期:2017-09-04 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-017-0316-1
更新日期:2017-01-17 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256111
更新日期:2003-09-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256421
更新日期:1999-01-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255919
更新日期:2000-01-01 00:00:00
abstract::Glycine N-methyltransferase (GNMT) is a protein with multiple functions. Recently, two Italian siblings who had hepatomegaly and chronic elevation of serum transaminases were diagnosed to have GNMT deficiency caused by inherited compound heterozygosity of the GNMT gene with missence mutations. To evaluate the expressi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256001
更新日期:2003-01-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9114-6
更新日期:2006-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-80
更新日期:2009-09-02 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-020-00680-9
更新日期:2020-08-19 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-0621-y
更新日期:2020-01-22 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-20-49
更新日期:2013-07-12 00:00:00
abstract::The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a sign...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253367
更新日期:2000-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-63
更新日期:2010-07-28 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:2016-01-19 00:00:00
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journal_title:Journal of biomedical science
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doi:10.1186/1423-0127-16-22
更新日期:2009-02-19 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-85
更新日期:2009-09-21 00:00:00
abstract::Although hypercholesterolemia is a well-known risk factor for atherosclerosis, little is known about the effect of hypercholesterolemia on cardiac contractile function. The objective of this study was to examine the effect of hypercholesterolemia on myocardial contractility. Fifteen New Zealand white rabbits were fed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254368
更新日期:2004-11-01 00:00:00
abstract::The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-004-8166-8
更新日期:2005-01-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9146-6
更新日期:2007-05-01 00:00:00
abstract::The HIV-1 Tat protein is an RNA-binding transcriptional transactivator. Recent findings suggest that Tat associates with a cellular kinase that phosphorylates the carboxyl-terminal domain of the largest subunit of RNA polymerase II. Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcrip...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/BF02253352
更新日期:1998-01-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256622
更新日期:2000-03-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-18-78
更新日期:2011-10-27 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00636-z
更新日期:2020-04-07 00:00:00
abstract::The isoprostanes (IsoPs) are a unique series of prostaglandin-like compounds formed in vivo from the free radical-catalyzed peroxidation of arachidonic acid. This review summarizes our current knowledge regarding these compounds. Novel aspects of the biochemistry and bioactivity of IsoPs are detailed and methods by wh...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/BF02253564
更新日期:1999-07-01 00:00:00