Abstract：
:To identify novel genes associated with apoptosis in glioma cells, we treated T98G glioma cells with okadaic acid (OA). Differential display using 15 random primers was performed on RNA extracted from these cells. Upregulated bands were excised from polyacrylamide gels and cloned. Northern blots were used to confirm RNA expression in T98G cells. 18 RNA fragments corresponding to the untranslated region of genes were identified and sequenced. Three unknown gene fragments were used to screen a fetal brain cDNA library resulting in three complete cDNA sequences. The three sequences corresponded to a human gene homologous to the yeast translation initiation factor Sui-1, a cAMP-regulated phosphoprotein, ARPP-16/19, and a novel gene designated O48. Transcription of Sui-1 increased in response to all stress factors tested, whereas ARPP only responded to OA. 2-kb and 4-kb O48 RNA species were identified. OA and stress factors increased 2-kb expression while K252a (protein kinase inhibitor) increased 4-kb expression. Differential display is effective for identifying genes associated with apoptosis. Novel genes may be identified by further analysis of the gene fragments identified in this study. The function of O48 is unknown.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chin LS,Singh SK,Wang Q,Murray SFdoi
10.1007/BF02256622keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
152-9issue
2eissn
1021-7770issn
1423-0127pii
25441journal_volume
7pub_type
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journal_title：Journal of biomedical science
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journal_title：Journal of biomedical science
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更新日期：2017-08-17 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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更新日期：2009-10-22 00:00:00
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journal_title：Journal of biomedical science
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journal_title：Journal of biomedical science
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更新日期：1999-01-01 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2009-02-05 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256573
更新日期：2002-01-01 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2008-01-01 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2009-03-25 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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更新日期：2009-01-19 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2015-05-28 00:00:00
