Abstract:
:Fibroblasts derived from glucose-6-phosphate dehydrogenase (G6PD)-deficient patients display retarded growth and accelerated cellular senescence that is attributable to increased accumulation of oxidative DNA damage and increased sensitivity to oxidant-induced senescence, but not to accelerated telomere attrition. Here, we show that ectopic expression of hTERT stimulates telomerase activity and prevents accelerated senescence in G6PD-deficient cells. Stable clones derived from hTERT-expressing normal and G6PD-deficient fibroblasts have normal karyotypes, and display no sign of senescence beyond 145 and 105 passages, respectively. Activation of telomerase, however, does not prevent telomere attrition in earlier-passage cells, but does stabilize telomere lengths at later passages. In addition, we provide evidence that ectopic expression of hTERT attenuates the increased sensitivity of G6PD-deficient fibroblasts to oxidant-induced senescence. These results suggest that ectopic expression of hTERT, in addition to acting in telomere length maintenance by activating telomerase, also functions in regulating senescence induction.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Wu YH,Cheng ML,Ho HY,Chiu DT,Wang TCdoi
10.1186/1423-0127-16-18subject
Has Abstractpub_date
2009-02-05 00:00:00pages
18eissn
1021-7770issn
1423-0127pii
1423-0127-16-18journal_volume
16pub_type
杂志文章abstract:BACKGROUND:Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. METHODS:Induced keratinocyte-like cells (iKCs) were directly reprogrammed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2020-04-20 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2012-07-31 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Journal of biomedical science
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doi:10.1186/s12929-019-0511-3
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-015-0194-3
更新日期:2015-10-15 00:00:00
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journal_title:Journal of biomedical science
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更新日期:1996-04-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2017-01-17 00:00:00
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更新日期:2020-02-08 00:00:00
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journal_title:Journal of biomedical science
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abstract::The HIV-1 Tat protein is an RNA-binding transcriptional transactivator. Recent findings suggest that Tat associates with a cellular kinase that phosphorylates the carboxyl-terminal domain of the largest subunit of RNA polymerase II. Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcrip...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2018-09-04 00:00:00
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2014-08-02 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2018-11-19 00:00:00