Abstract:
BACKGROUND:Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. METHODS:Induced keratinocyte-like cells (iKCs) were directly reprogrammed from human urine cells by retroviral transduction of two lineage-specific transcription factors BMI1 and △NP63α (BN). Expression of keratinocyte stem cell or their differentiation markers were assessed by PCR, immunofluorescence and RNA-Sequencing. Regeneration capacity of iKCs were assessed by reconstitution of a human skin equivalent under air-interface condition. RESULTS:BN-driven iKCs were similar to primary keratinocytes (pKCs) in terms of their morphology, protein expression, differentiation potential, and global gene expression. Moreover, BN-iKCs self-assembled to form stratified skin equivalents in vitro. CONCLUSIONS:This study demonstrated an approach to generate human iKCs that could be directly reprogrammed from human somatic cells and extensively expanded in serum- and feeder cell-free systems, which will facilitate their broad applicability in an efficient and patient-specific manner.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Zheng J,Yun W,Park J,Kang PJ,Lee G,Song G,Kim IY,You Sdoi
10.1186/s12929-020-00642-1subject
Has Abstractpub_date
2020-04-20 00:00:00pages
56issue
1eissn
1021-7770issn
1423-0127pii
10.1186/s12929-020-00642-1journal_volume
27pub_type
杂志文章abstract::Although hypercholesterolemia is a well-known risk factor for atherosclerosis, little is known about the effect of hypercholesterolemia on cardiac contractile function. The objective of this study was to examine the effect of hypercholesterolemia on myocardial contractility. Fifteen New Zealand white rabbits were fed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254368
更新日期:2004-11-01 00:00:00
abstract::Influence of adjuvants i.e., alpha-tocopherol (25 mg/kg, p.o.) and piperine (10 mg/kg, p.o.) on therapeutic potential of chelator tiferron (300 mg/kg, i.p.) was evaluated to encounter toxicogenic events of beryllium exposure. Albino rats were exposed to beryllium nitrate (1 mg/kg, i.p.) daily for 28 days followed by t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9147-5
更新日期:2007-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S34
更新日期:2010-08-24 00:00:00
abstract:BACKGROUND:Mounting evidences have showed the important role of transforming growth factor-β (TGF-β) in immunological surveillance of tumors. Some studies have also indicated human leukocyte antigen (HLA)-G-associated immune escape involving TGF-β management in gastric cancer (GC). However, the mechanism underlying it ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0177-4
更新日期:2015-12-02 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0165-8
更新日期:2015-07-15 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253343
更新日期:1994-06-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0551-8
更新日期:2019-08-07 00:00:00
abstract::The isoprostanes (IsoPs) are a unique series of prostaglandin-like compounds formed in vivo from the free radical-catalyzed peroxidation of arachidonic acid. This review summarizes our current knowledge regarding these compounds. Novel aspects of the biochemistry and bioactivity of IsoPs are detailed and methods by wh...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:1999-07-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-17
更新日期:2012-02-07 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255935
更新日期:1998-11-01 00:00:00
abstract::Adenosine was shown to inhibit norepinephrine (NE) release from sympathetic nerve endings. The purpose of this study was to examine whether endogenous adenosine restrains NE and epinephrine release from the adrenal medulla. The effects of an adenosine receptor antagonist, 1,3-dipropyl-8-(p-sulfophenyl) xanthine (DPSPX...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255947
更新日期:2001-09-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Journal of biomedical science
pub_type: 已发布勘误
doi:10.1186/s12929-020-00686-3
更新日期:2020-09-21 00:00:00
abstract::Dextromethorphan (DM), an antitussive agent, has been claimed to have anti-inflammatory and immunomodulatory effects in vitro. Thus, the aim of this study was to evaluate the effects of DM on sepsis induced by intravenous (i.v.) administration of lipopolysaccharide (LPS) in anesthetized Wistar rats and by intraperiton...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254358
更新日期:2004-11-01 00:00:00
abstract::In all eukaryotes, the plasma membrane is critically important as it maintains the architectural integrity of the cell. Proper anchorage and interaction between the plasma membrane and the cytoskeleton is critical for normal cellular processes. The ERM (ezrin-radixin-moesin) proteins are a class of highly homologous p...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0246-3
更新日期:2016-03-17 00:00:00
abstract:BACKGROUND:Cisplatin (cis-diamminedichloroplatinum II, CDDP) is one of the most effective chemotherapeutic agents. However, its clinical use is limited due to the severe side effects, including nephrotoxicity and acute kidney injury (AKI) which develop due to renal accumulation and biotransformation of CDDP. The allevi...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0518-9
更新日期:2019-03-13 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0287-7
更新日期:2016-10-06 00:00:00
abstract::Glucocorticoids regulate the expression of many liver-specific genes via glucocorticoid receptors. The presence of glucocorticoid receptors in liver has been reported in many mammalian species but not in nude mice. In the present study, we demonstrate the presence of specific glucocorticoid receptors in nude mouse liv...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253306
更新日期:1994-10-01 00:00:00
abstract:BACKGROUND:Increased levels of blood plasma urea were used as phenotypic parameter for establishing novel mouse models for kidney diseases on the genetic background of C3H inbred mice in the phenotype-driven Munich ENU mouse mutagenesis project. The phenotypically dominant mutant line HST014 was established and further...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-017-0365-5
更新日期:2017-08-17 00:00:00
abstract:BACKGROUND:This study aimed at developing nerve growth factor (NGF) mimetics that selectively activate specific biological signals and, as a result, lack the side effects of the full-length protein. Two dimeric dipeptides, bis-(N-aminocaproyl-glycyl-L-lysine) hexamethylenediamide (GK-6) and bis(N-succinyl-L-glutamyl-L-...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0198-z
更新日期:2015-12-08 00:00:00
abstract::The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransp...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-100
更新日期:2009-11-16 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000073968
更新日期:2003-11-01 00:00:00
abstract::To determine the involvement of the globus pallidus in mediating epilepsy, the effects of microinjection of a GABA uptake blocker (tiagabine), a benzodiazepine agonist (zolpidem) and a GABA-B receptor agonist (baclofen) on pentylenetetrazol (PTZ)-induced tonic seizure were examined in adult rats. Administration of PTZ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256094
更新日期:2004-07-01 00:00:00
abstract::Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles that serve a means of cell-cell communication. Studying EVs at a single-particle level is important because EVs are inherently heterogeneous. Novel micro- and nanotechnological tools have open opportunities for realizing single-EV measurements explo...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-019-0502-4
更新日期:2019-01-15 00:00:00
abstract:BACKGROUND:Next-generation sequencing (NGS) is a powerful and high-throughput method for the detection of viral mutations. This article provides a brief overview about optimization of NGS analysis for hepatocellular carcinoma (HCC)-associated hepatitis B virus (HBV) mutations, and hepatocarcinogenesis of relevant mutat...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-018-0442-4
更新日期:2018-06-02 00:00:00
abstract:BACKGROUND:Developing brain is a major target for alcohol's actions and neurological/functional abnormalities include microencephaly, reduced frontal cortex, mental retardation and attention-deficits. Previous studies have shown that ethanol altered the lateral ventricular neuroepithelial cell proliferation. However, t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-016-0225-8
更新日期:2016-01-19 00:00:00
abstract::Adeno-associated virus (AAV)-2 was developed as a useful vector for human gene therapy. In this report, we analyzed the integration and expression of AAV-mediated ex vivo transferred human beta-globin gene in bone marrow (BM) reconstituted mice. Recombinant AAV (rAAV) containing human beta-globin gene was packaged by ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256072
更新日期:2002-05-01 00:00:00
abstract::The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256432
更新日期:2003-07-01 00:00:00
abstract::Endotoxin shock is characterized by systemic hypotension, hyporeactiveness to vasoconstrictors and acute lung edema. A nitric oxide synthase (NOS) inhibitor, NG-monomethyl-L-arginine (L-NMMA) has been shown to be effective in reversing acute lung injury. In the present study, we evaluated the effects of NOS blockade b...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256421
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Interleukin 12B (IL12B) gene polymorphisms have been linked to several inflammatory diseases, but their role in the development of Graves ophthalmopathy (GO) in Graves disease (GD) patients is unclear. The purpose of this study was to investigate the disease association of IL12B single nucleotide polymorphis...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-97
更新日期:2012-11-19 00:00:00
abstract::Oxidative stress and inflammation contributed to the propagation of acute liver injury (ALI). The present study was undertaken to determine whether D-galactosamine (D-GalN) induces ALI via the mitochondrial apoptosis- and proinflammatory cytokine-signaling pathways, and possible mechanism(s) by which green tea (GT) ex...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-35
更新日期:2009-03-25 00:00:00