Abstract:
:Dehydroepiandrosterone (DHEA) is a putative anti-stress agent and stress is associated with the secretion of catecholamine from the adrenal gland, but the effects of DHEA on catecholamine secretion are not fully understood. Using bovine chromaffin cells, we found that DHEA inhibited catecholamine secretion and cytosolic Ca(2+) ([Ca(2+)](i)) rise coupled with nicotinic acetylcholine receptor (nAChR) without exerting an effect on (3)H-nicotine binding. In the case of high K(+) stimulation, DHEA effectively suppressed secretion without affecting [Ca(2+)](i) rise. Trifluoperazine (TFP), a calmodulin inhibitor, was capable of counteracting the inhibition of DHEA on high K(+)-induced secretions. In permeabilized cells, DHEA suppressed the Ca(2+)-induced secretion. These results suggest that DHEA (a) acts as a channel blocker that suppresses Ca(2+) influx and subsequent secretions associated with nAChR, or (b) affects the intracellular secretion machinery to suppress high K(+)-induced secretions without affecting the high K(+)-induced [Ca(2+)](i) rise.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Liu PS,Wang PYdoi
10.1007/BF02256563keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
200-5issue
2eissn
1021-7770issn
1423-0127pii
76032journal_volume
11pub_type
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