Abstract:
BACKGROUND:Adipogenesis is a complex process that involves many genes/proteins at different stages of differentiation. In order to identify genes critical for adipogenesis, we took a novel approach based on phenotype change of individual cell, to search for genes with regulatory roles in adipogenesis genome-wide in 3T3-L1 cells. METHODS:Lentivirus-based inducible random homologous knockdown was used for the screening of functional gene that altered lipid formation in the adipocyte during differentiation. RESULTS:In the present study, we reported the identification of an alternatively spliced mitochondrial oxodicarboxylate carrier (ODC), so named ODC-AS. ODC-AS is different from ODC by replacing 22 amino acids with 29 amino acids at the N-terminal. ODC was widely expressed in most tissues in mouse as determined by multi-tissue cDNA panel polymerase chain reaction. However, ODC-AS was only detected in adipose tissue and in iris and sclera-choroid complex of the eye. The expression of ODC-AS in 3T3-L1 was detected after the induction of differentiation, and reached a peak at day 4 and then reduced thereafter, whereas no ODC transcript detected in the cells neither before nor after differentiation. Knocking down of ODC-AS expression by RNA interference led to significant reduction in lipid accumulation as determined by triglyceride measurement and Nile Red staining, as well as adipogenic marker CEBPalpha, PPARgamma, aP2 and CD36. Although both ODC and ODC-AS are expressed in white and brown adipose tissues, only the expression of ODC-AS was down-regulated in brown adipose tissue by cold exposure. CONCLUSION:These results implicate that ODC-AS may promote lipid accumulation during adipocyte differentiation and play an important role in the regulation of lipid metabolism in adipose tissues.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Niimi M,Tao L,Lin SH,Yin J,Wu X,Fukui H,Kambayashi J,Ye J,Sun Bdoi
10.1186/1423-0127-16-92subject
Has Abstractpub_date
2009-10-13 00:00:00pages
92eissn
1021-7770issn
1423-0127pii
1423-0127-16-92journal_volume
16pub_type
杂志文章abstract::The ACTGCTGA sequence (CTG motif) is located immediately upstream of the NF-kappaB enhancer in the human immunodeficiency virus type-1 (HIV-1) long terminal repeat (LTR). We previously reported on the frequent duplication of this motif in HIV-1-infected individuals. In this study we further characterized the role of t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0534-9
更新日期:2019-06-06 00:00:00
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pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00647-w
更新日期:2020-04-02 00:00:00
abstract::Due to high prevalence and mortality and the lack of effective therapies, prostate cancer is one of the most crucial health problems in men. Drug resistance aggravates the situation, not only in human prostate cancer but also in other cancers. In this study, we report for the first time that cardiac glycosides (e.g. o...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256539
更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0190-7
更新日期:2015-10-20 00:00:00
abstract:BACKGROUND:Promoting post-stroke neurogenesis has long been proposed to be a therapeutic strategy for the enhancement of functional recovery after cerebral ischemic stroke. Despite numerous approaches have been widely reported the proliferation or differentiation of the neurogenic population therapeutic strategies by t...
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doi:10.1186/s12929-019-0521-1
更新日期:2019-04-18 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256602
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12929-015-0198-z
更新日期:2015-12-08 00:00:00
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pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
abstract::Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by degeneration of lower motor neurons. We have assayed deletions in two candidate genes, the survival motor neuron (SMN) and neuronal apoptosis inhibitory protein (NAIP) genes, in 108 samples, of which 46 were from SMA patients, and 62 wer...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256412
更新日期:2001-03-01 00:00:00
abstract::Lysosome-associated membrane protein (LAMP)-1, one of the major protein components of the lysosomal membrane, is upregulated in the human glioblastoma cell lines, U-373 MG and LN-Z308, which undergo cisplatin-induced apoptosis. These human brain tumor cell lines demonstrated apoptosis in response to cisplatin/nifedipi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258379
更新日期:2001-07-01 00:00:00
abstract::Retrograde transport of Wheat germ agglutinin conjugated to Horseradish peroxidase (WGA-HRP) was used in labeling vagal neurons projecting to the stomach from the dorsal motor nucleus of the vagus nerve (DMNV) in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in the experimental rats by intraperitone...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9097-3
更新日期:2006-09-01 00:00:00
abstract::Reactive oxygen species (ROS) have been long considered simply as harmful by-products of metabolism, which damage cellular proteins, lipids, and nucleic acids. ROS are also known as a weapon of phagocytes, employed against pathogens invading the host. However, during the last decade, an understanding has emerged that ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-015-0194-3
更新日期:2015-10-15 00:00:00
abstract::Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/s11373-005-9063-5
更新日期:2006-03-01 00:00:00
abstract::Mitochondria are sub-cellular organelles that produce adenosine triphosphate (ATP) through oxidative phosphorylation (OXPHOS). As suggested over 70 years ago by Otto Warburg and recently confirmed with molecular techniques, alterations in respiratory activity and in mitochondrial DNA (mtDNA) appear to be common featur...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/1423-0127-17-31
更新日期:2010-04-25 00:00:00
abstract::Over the past few years, long non-coding RNAs (lncRNAs) are recognized as key regulators of gene expression at chromatin, transcriptional and posttranscriptional level with pivotal roles in various biological and pathological processes, including cancer. Hypoxia, a common feature of the tumor microenvironment, profoun...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00654-x
更新日期:2020-05-05 00:00:00
abstract:BACKGROUND:Interleukin 12B (IL12B) gene polymorphisms have been linked to several inflammatory diseases, but their role in the development of Graves ophthalmopathy (GO) in Graves disease (GD) patients is unclear. The purpose of this study was to investigate the disease association of IL12B single nucleotide polymorphis...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-97
更新日期:2012-11-19 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-83
更新日期:2009-09-14 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254441
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:The association of TGF beta1 polymorphisms and atrial fibrillation (AF) in essential hypertensive (EH) subjects remains unknown. Methods EH subjects with AF (EH+AF+) and sinus rhythm (EH+AF-) were enrolled. The polymorphisms of +869 T --> C at codon 10 and + 915 G --> C at codon 25, were genotyped. The clini...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-23
更新日期:2010-03-31 00:00:00
abstract::Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256452
更新日期:2003-05-01 00:00:00
abstract::The dual signal hypothesis of apoptosis holds that a common signal can activate both apoptotic and proliferative pathways. The fate of a cell is dependent on which of these two pathways predominates. In the MAPK family of kinases, ERK and JNK have been proposed to mediate apoptosis whereas the PI3K-stimulated kinase, ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255905
更新日期:1999-05-01 00:00:00
abstract::We previously reported that transgenic ablation of gonadotrophs results in impaired development of cells immunostainable for prolactin (PRL) but not of cells immunostainable for growth hormone (GH) or pro-opiomelanocortin (POMC) in pituitary of newborn mice. The question remained whether this reduction in PRL protein ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000073968
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:Cell adhesion has been shown to induce activation of certain growth factor receptors in a ligand-independent manner. However, the mechanism for such activation remains obscure. METHODS:Human epidermal carcinoma A431 cells were used as a model to examine the mechanism for adhesion-induced activation of hepat...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-18-78
更新日期:2011-10-27 00:00:00
abstract:BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0602-1
更新日期:2020-02-08 00:00:00
abstract::The Peutz-Jeghers syndrome (PJS) is a hereditary disorder that predisposes an individual to benign and malignant tumors in multiple organ systems. Recently, the locus responsible for PJS was mapped genetically to the LKB1 gene, with a subsequent investigation proving that it is responsible for most cases of PJS. LKB1 ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256059
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Helicobacter pylori bacteria colonize human gastric mucosa, cause chronic inflammation, peptic ulcers and gastric cancer. Colonization is mediated by H. pylori adhesins, which preferentially bind mucin 5 (MUC5AC) and Lewis (Le) determinants. The aim of this study was to evaluate the influence of H. pylori an...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0515-z
更新日期:2019-03-06 00:00:00
abstract:BACKGROUND:Chemotherapy and targeted therapies have made important strides in cancer treatment yet they often fail and new therapies are still needed. Here, we employed a phenotypic screen to identify and analyze the mechanism of action of novel small molecules that interfere with critical pathways involved in tumor ce...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0408-6
更新日期:2018-02-08 00:00:00
abstract::Recent transcriptome analyses have revealed that noncoding RNAs (ncRNAs) are broadly expressed in mammalian cells and abundant in the CNS, with tissue and cell type-specific expression patterns. Moreover, ncRNAs have been found to intricately and dynamically regulate various signaling pathways in neurodegeneration. As...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-020-00636-z
更新日期:2020-04-07 00:00:00