Abstract:
:Induction of tumoricidal activity is one of the major functions of activated macrophages. Our previous study demonstrated that P388D1 murine macrophage-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent studies demonstrated that PCF killed MOPC-315 cells by induction of apoptosis. In this report, the involvement of Fas and Fas ligand (FasL) in PCF-induced apoptosis was investigated. Results suggest that expression of Fas mRNA time-dependently increased in PCF-treated cells and reached an optimal level after 36 h of treatment. The augmented effect of PCF on Fas mRNA expression was significantly reduced by the addition of CB7.C2, an anti-PCF monoclonal antibody. The expression of FasL mRNA was also induced by PCF and reached an optimal level at 24 h, but sharply decreased after 36 h of treatment. Caspase-3 is one of the proteolytic enzymes that can be activated by the Fas-FasL interaction. In our studies, the enzymatic activity of caspase-3 was significantly induced by PCF after 6 h of treatment and reached an optimal level at 12 h. The augmented effect of PCF on caspase activity was significantly reduced by the addition of CB7.C2 and the caspase-3-specific inhibitor, DEVD-fmk. Therefore, PCF-treated plasmacytoma cells might undergo apoptosis via interaction between Fas and FasL.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chu CY,Tseng Jdoi
10.1007/BF02255919keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
58-63issue
1eissn
1021-7770issn
1423-0127pii
25430journal_volume
7pub_type
杂志文章abstract::In this study, we examined the postnatal expression patterns of p63 and other keratinocyte stem cell markers in the rat cornea in an attempt to determine the markers that best represent characteristics of corneal keratinocyte stem cells. We show that the expression of p63 in the rat cornea is unique and differs from t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2014-04-17 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-03-26 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1186/1423-0127-17-1
更新日期:2010-01-07 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-20-49
更新日期:2013-07-12 00:00:00
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pub_type: 杂志文章
doi:10.1186/1423-0127-17-23
更新日期:2010-03-31 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11373-006-9114-6
更新日期:2006-11-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:2016-03-17 00:00:00
abstract::Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by degeneration of lower motor neurons. We have assayed deletions in two candidate genes, the survival motor neuron (SMN) and neuronal apoptosis inhibitory protein (NAIP) genes, in 108 samples, of which 46 were from SMA patients, and 62 wer...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256412
更新日期:2001-03-01 00:00:00
abstract::Over the past few years, long non-coding RNAs (lncRNAs) are recognized as key regulators of gene expression at chromatin, transcriptional and posttranscriptional level with pivotal roles in various biological and pathological processes, including cancer. Hypoxia, a common feature of the tumor microenvironment, profoun...
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pub_type: 杂志文章,评审
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更新日期:2020-05-05 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2002-05-01 00:00:00
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更新日期:2018-11-19 00:00:00
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pub_type: 杂志文章
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更新日期:2015-07-15 00:00:00
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pub_type: 杂志文章
doi:10.1186/1423-0127-17-51
更新日期:2010-06-24 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2002-11-01 00:00:00
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of biomedical science
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
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更新日期:2019-10-23 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-020-00642-1
更新日期:2020-04-20 00:00:00