Abstract：
:Based on the tertiary structure of the ribosome-inactivating protein alpha-sarcin, domains that are responsible for hydrolyzing ribosomes and naked RNA have been dissected. In this study, we found that the head-to-tail interaction between the first amino beta-strand and the last carboxyl beta-strand is not involved in catalyzing the hydrolysis of ribosomes or ribonucleic acids. Instead, a four-strand pleated beta-sheet is indispensable for catalyzing both substrates, suggesting that alpha-sarcin and ribonuclease T1 (RNase T1) share a similar catalytic center. The integrity of an amino beta-hairpin and that of the loop L3 in alpha-sarcin are crucial for recognizing and hydrolyzing ribosomes in vitro and in vivo. However, a mutant protein without the beta-hairpin structure, or with a disrupted loop L3, is still capable of digesting ribonucleic acids. The functional involvement of the beta-hairpin and the loop L3 in the sarcin stem/loop RNA of ribosomes is demonstrated by a docking model, suggesting that the two structures are in essence naturally designed to distinguish ribosome-inactivating proteins from RNase T1 to inactivate ribosomes.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Hwu L,Huang K,Chen D,Lin Adoi
10.1007/BF02255817keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
420-8issue
5eissn
1021-7770issn
1423-0127pii
25477journal_volume
7pub_type
杂志文章abstract：BACKGROUND:The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus, it is of great interest to look for an...
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pub_type： 杂志文章
doi：10.1186/1423-0127-18-42
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更新日期：2010-08-24 00:00:00
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更新日期：2003-07-01 00:00:00
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更新日期：2012-04-30 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章,多中心研究
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更新日期：2012-01-03 00:00:00
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更新日期：2012-11-19 00:00:00
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journal_title：Journal of biomedical science
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doi：10.1007/BF02256609
更新日期：2001-11-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9147-5
更新日期：2007-05-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-7212-5
更新日期：2005-01-01 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-20-34
更新日期：2013-05-31 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-004-8166-8
更新日期：2005-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-08-07 00:00:00
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journal_title：Journal of biomedical science
pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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更新日期：1994-03-01 00:00:00
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更新日期：1994-10-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Journal of biomedical science
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更新日期：2000-03-01 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2009-01-01 00:00:00
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更新日期：2005-01-01 00:00:00
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journal_title：Journal of biomedical science
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更新日期：2009-02-19 00:00:00
abstract：:Sequencing of HIV-1(GER) long terminal repeat (LTR) has demonstrated, for the first time in an HIV-1 primary isolate, a TAR duplication referred to as TAR1 (nucleotides +1 through +68) and TAR2 (nucleotides +69 through +136). This TAR duplication is stable during replication of HIV-1(GER) isolate in CEM cells. Analysi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253576
更新日期：1996-01-01 00:00:00
abstract：:Metabolic diseases such as obesity, type 2 diabetes (T2D) and insulin resistance have attracted great attention from biomedical researchers and clinicians because of the astonishing increase in its prevalence. Decrease in the capacity of oxidative metabolism and mitochondrial dysfunction are a major contributor to the...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
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更新日期：2017-09-07 00:00:00
