Abstract:
:The intracellular mechanisms underlying oxidized low-density lipoprotein (oxLDL)-signaling pathways in platelets are not yet completely understood. Therefore, the aim of this study was to further examine the effects of oxLDL in prevention of platelet aggregation. In this study, oxLDL concentration-dependently (40-120 microg/ml) inhibited platelet aggregation in human platelet-rich plasma stimulated by agonists. Moreover, oxLDL (40 and 80 microg/ml) markedly decreased the fluorescence intensity of platelet membranes tagged with diphenylhexatriene. Rapid phosphorylation of a protein of Mr 47,000 (P47), a marker of protein kinase C activation, was triggered by PDBu (150 nM). This phosphorylation was markedly inhibited by oxLDL (40 and 80 microg/ml) in phosphorus-32-labeled platelets. In addition, oxLDL (40 and 80 microg/ml) markedly increased levels of cyclic AMP and cyclic AMP-induced vasodilator-stimulated phosphoprotein (VASP) Ser(157) phosphorylation. The thrombin-evoked increase in pHi was inhibited in the presence of oxLDL (40 and 80 microg/ml). These results indicate that the antiplatelet activity of oxLDL may involve the following pathways. (1) oxLDL may initially induce conformational changes in platelet membranes, leading to inhibition of the activation of protein kinase C, followed by inhibition of P47 protein phosphorylation, and intracellular Ca(2+) mobilization. (2) oxLDL also activated formation of cyclic AMP and cyclic AMP-induced VASP Ser(157) phosphorylation, resulting in inhibition of the Na(+)/H(+)exchanger; this leads to reduced intracellular Ca(2+) mobilization, and ultimately to inhibition of platelet aggregation. This study further provides new insights concerning the effects of low concentrations of oxLDL on platelet aggregation.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Chou DS,Chan CH,Hsiao G,Shen MY,Tsai YJ,Chen TF,Sheu JRdoi
10.1007/s11373-005-9042-xkeywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
333-43issue
3eissn
1021-7770issn
1423-0127journal_volume
13pub_type
杂志文章abstract::The nuclear envelope (NE) consists of two membrane layers that segregate the nuclear from the cytoplasmic contents. Recent progress in our understanding of nuclear-lamina associated diseases has revealed intriguing connections between the envelope components and nuclear processes. Here, we review the functions of the ...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/1423-0127-16-96
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更新日期:2004-07-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:1998-11-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2015-12-08 00:00:00
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journal_title:Journal of biomedical science
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更新日期:1996-04-01 00:00:00
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更新日期:1995-01-01 00:00:00
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更新日期:2005-01-01 00:00:00
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更新日期:2013-02-23 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
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更新日期:2019-08-07 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0526-9
更新日期:2019-05-11 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-44
更新日期:2009-05-01 00:00:00
abstract::Experimental allergic encephalomyelitis (EAE) is an accepted animal model for the human demyelinating disease multiple sclerosis. The continuously propagated line of Lewis rat T helper lymphocytes (GP1 T cells), specific for the encephalitogenic 68-86 sequence of guinea pig myelin basic protein (GPMBP), mediates the a...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255600
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND:Mounting evidences have showed the important role of transforming growth factor-β (TGF-β) in immunological surveillance of tumors. Some studies have also indicated human leukocyte antigen (HLA)-G-associated immune escape involving TGF-β management in gastric cancer (GC). However, the mechanism underlying it ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0177-4
更新日期:2015-12-02 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258364
更新日期:1998-01-01 00:00:00
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journal_title:Journal of biomedical science
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doi:10.1186/1423-0127-16-83
更新日期:2009-09-14 00:00:00
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doi:10.1186/s12929-016-0257-0
更新日期:2016-04-16 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-04-07 00:00:00
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journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-8
更新日期:2010-02-12 00:00:00
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journal_title:Journal of biomedical science
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更新日期:1994-03-01 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2017-01-05 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2010-07-28 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2009-10-13 00:00:00
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journal_title:Journal of biomedical science
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更新日期:2018-11-19 00:00:00
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journal_title:Journal of biomedical science
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