Abstract:
BACKGROUND:Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. RESULTS:The transfection of CS-pEGFP NPs was efficient in CHO cells and the expression of green fluorescent proteins was well observed. In addition, NCS-DNA E7 vaccine induced the strongest E7-specific CD8+ T cell and interferon γ responses in C57BL/6 mice. Mice vaccinated with NCS-DNA E7 vaccine were able to generate potent protective and therapeutic antitumor effects against challenge with E7-expressing tumor cell line, TC-1. CONCLUSIONS:The strong therapeutic effect induced by the Chitosan-based nanodelivery suggest that nanoparticles may be an efficient carrier to improve the immunogenicity of DNA vaccination upon intramuscular administration and the platform could be further exploited as a potential cancer vaccine candidate in humans.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Tahamtan A,Ghaemi A,Gorji A,Kalhor HR,Sajadian A,Tabarraei A,Moradi A,Atyabi F,Kelishadi Mdoi
10.1186/s12929-014-0069-zsubject
Has Abstractpub_date
2014-07-31 00:00:00pages
69eissn
1021-7770issn
1423-0127pii
s12929-014-0069-zjournal_volume
21pub_type
杂志文章abstract::Plasma nitric oxide (NO) levels in Duchenne muscular dystrophy (DMD) patients were significantly lower than those observed in both healthy controls and in patients with other neuromuscular disorders. The correlation between NO level and ejection fraction was significant (r = -0.384, p = 0.0391) in the DMD group. Disru...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256104
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Loss of ovarian function, as in menopause or after ovariectomy (OVX), is closely associated with obesity and white adipose tissue (WAT) inflammation. Estrogen replacement protects against postmenopausal obesity but increases the risks of carcinogenesis. In the present study, we investigated the effects of lo...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0556-3
更新日期:2019-08-31 00:00:00
abstract::Protein kinases play pivotal roles in the development of the nervous system. They are involved in almost every stage of neuronal development, from initial proliferation and differentiation of progenitor cells to pathfinding of neurites and formation of synapses. Activation of protein kinases is also critical for neuro...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258364
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Stroke is one of the leading causes of neuronal death. Sesamin is known for neuroprotection by its antioxidant and anti-inflammatory properties but it lacks blood-brain barrier (BBB) activity. A panel of sesamin derivatives was screened and 3-bis (3-methoxybenzyl) butane-1,4-diol (BBD) was selected for high ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-21-15
更新日期:2014-02-18 00:00:00
abstract::Adeno-associated virus (AAV) vectors were shown capable of high efficiency transduction of both dividing and nondividing cells and tissues. AAV-mediated transduction leads to stable, long-term transgene expression in the absence of apparent immune response. These properties and the broad host range of AAV vectors indi...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/BF02253246
更新日期:2000-07-01 00:00:00
abstract::The Tat proteins of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2), termed Tat-1 and Tat-2, respectively, are essential for efficient viral replication. Tat proteins activate viral transcription by binding to the TAR RNA stem-loop structure at the 5' end of viral transcripts. We used an in vitro selection ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255590
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. METHODS:Induced keratinocyte-like cells (iKCs) were directly reprogrammed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-020-00642-1
更新日期:2020-04-20 00:00:00
abstract::Two Chinese hamster ovary (CHO) cell lines stably transfected with human insulin receptor cDNA, CHO-wt and CHO-mut, which express an equivalent number of normal and kinase-defective human insulin receptors, respectively, were used to assess the roles of insulin receptor tyrosine kinase activity in insulin-regulated ge...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258333
更新日期:1994-12-01 00:00:00
abstract::Human mesenchymal stem cells (MSCs) are multilineage somatic progenitor/stem cells that have been shown to possess immunomodulatory properties in recent years. Initially met with much skepticism, MSC immunomodulation has now been well reproduced across tissue sources and species to be clinically relevant. This has ope...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0289-5
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of mono...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-020-00694-3
更新日期:2020-11-30 00:00:00
abstract:BACKGROUND:Fallopian tube epithelial cells (FTEC) were thought to be the origin of high-grade serous ovarian carcinoma (HGSOC). Knowledge of the stemness or initiating characteristics of FTEC is insufficient. Previously, we have characterized the stemness cell marker of FTEC, this study aims to further characterize the...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-019-0602-1
更新日期:2020-02-08 00:00:00
abstract::The relationship between diet and colorectal cancer has been previously demonstrated and supported with strong epidemiological evidence. The role of genetic polymorphisms has, however, been less well elaborated upon. We conducted a hospital-based case-control study including 727 cases and 736 healthy controls to evalu...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-006-9139-x
更新日期:2007-03-01 00:00:00
abstract::The intracellular mechanisms underlying oxidized low-density lipoprotein (oxLDL)-signaling pathways in platelets are not yet completely understood. Therefore, the aim of this study was to further examine the effects of oxLDL in prevention of platelet aggregation. In this study, oxLDL concentration-dependently (40-120 ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9042-x
更新日期:2006-05-01 00:00:00
abstract:BACKGROUND:Epigallocatechin-3-gallate (EGCg) with its potent anti-oxidative capabilities is known for its beneficial effects ameliorating oxidative injury to cardiac cells. Although studies have provided convincing evidence to support the cardioprotective effects of EGCg, it remains unclear whether EGCg affect trans-me...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-20-86
更新日期:2013-11-19 00:00:00
abstract::After streptococcal pyrogenic exotoxin B (SPE B) induces apoptosis, its fate is unknown. Using confocal time-course microscopy at 37 degrees C, we detected green fluorescence 20 min after adding FITC-SPE B. Orange fluorescence, an indication of co-localization of SPE B with lysosomes which were labeled with a red fluo...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-007-9154-6
更新日期:2007-05-01 00:00:00
abstract::An isolated thoracic spinal cord of the neonatal rat in vitro spontaneously generates sympathetic nerve discharge (SND) at ~25 degrees C, but it fails in SND genesis at < or = 10 degrees C. Basal levels of the c-Fos expression in the spinal cords incubated at < or = 10 degrees C and ~25 degrees C were compared to dete...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-44
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Fat embolism syndrome (FES) associated with acute lung injury (ALI) is a clinical condition following long bone fracture. We have reported 14 victims due to ALI with FES. Our laboratory has developed an animal model that produced fat emboli (FE). The major purpose of this study was to test whether neutrophil...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-3
更新日期:2012-01-05 00:00:00
abstract::Critical assessment of experimental muscle-pain models resulting from maximal muscle contraction may provide a means of assessing hypersensitivity and the central nociceptive mechanisms involved in diffused muscle pain. The aim of the present study was, therefore, to investigate the patterns of nociceptive behavior an...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-6595-7
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:The probability of local tumor control after radiotherapy (RT) remains still miserably poor in pediatric rhabdomyosarcoma (RMS). Thus, understanding the molecular mechanisms responsible of tumor relapse is essential to identify personalized RT-based strategies. Contrary to what has been done so far, a correc...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-020-00683-6
更新日期:2020-08-27 00:00:00
abstract:BACKGROUND:Increased levels of blood plasma urea were used as phenotypic parameter for establishing novel mouse models for kidney diseases on the genetic background of C3H inbred mice in the phenotype-driven Munich ENU mouse mutagenesis project. The phenotypically dominant mutant line HST014 was established and further...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-017-0365-5
更新日期:2017-08-17 00:00:00
abstract:BACKGROUND:Poly(ADP-ribose) is a NAD+-requiring, DNA-repairing, enzyme playing a central role in pancreatic beta-cell death and in the development of endothelial dysfunction in humans and experimental animals. PARP activation is also relevant to the development of complications of diabetes. Hence, agents capable of inh...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-17-S1-S16
更新日期:2010-08-24 00:00:00
abstract::Ischemia and reperfusion (I/R) - induced injury has been described as one of the main factors that contribute to the observed morbidity and mortality in a variety of clinical entities, including myocardial infarction, ischemic stroke, cardiac arrest and trauma. An imbalance between oxygen demand and supply, within the...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1186/s12929-016-0220-0
更新日期:2016-01-19 00:00:00
abstract::Activation of hepatic stellate cells (HSCs) plays a crucial role in liver fibrogenesis. armepavine (Arm, C19H23O3N), an active compound from Nelumbo nucifera, has been shown to exert immunosuppressive effects on T lymphocytes and on lupus nephritic mice. The aim of this study was to investigate whether Arm could exert...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-16-78
更新日期:2009-09-02 00:00:00
abstract::Dehydroepiandrosterone (DHEA) is a putative anti-stress agent and stress is associated with the secretion of catecholamine from the adrenal gland, but the effects of DHEA on catecholamine secretion are not fully understood. Using bovine chromaffin cells, we found that DHEA inhibited catecholamine secretion and cytosol...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256563
更新日期:2004-03-01 00:00:00
abstract::Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/s11373-005-9063-5
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections....
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-018-0472-y
更新日期:2018-09-18 00:00:00
abstract:BACKGROUND:Zerumbone, a sesquiterpene compound isolated from subtropical ginger, Zingiber zerumbet Smith, has been documented to exert antitumoral and anti- inflammatory activities. In this study, we demonstrate that zerumbone induces apoptosis in human glioblastoma multiforme (GBM8401) cells and investigate the apopto...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-19-86
更新日期:2012-10-05 00:00:00
abstract:BACKGROUND:Dioscin, a typical steroid saponin, is isolated from Dioscorea nipponica Makino and Dioscorea zingiberensis Wright. It has estrogenic activity and many studies have also reported that dioscorea plants have an effect in preventing and treating osteoporosis. However, the molecular mechanisms underlying their e...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/1423-0127-21-30
更新日期:2014-04-17 00:00:00
abstract::Glucocorticoids regulate the expression of many liver-specific genes via glucocorticoid receptors. The presence of glucocorticoid receptors in liver has been reported in many mammalian species but not in nude mice. In the present study, we demonstrate the presence of specific glucocorticoid receptors in nude mouse liv...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02253306
更新日期:1994-10-01 00:00:00
abstract:BACKGROUND:Mutations in mitogen-activated protein kinase (MAPK) kinase 1 (MEK1) that occur during cell proliferation and tumor formation are well described. Information on the roles of MEK2 in these effects is still limited. We established a constitutive MEK2 transgenic zebrafish, Tg(krt14:MEK2S219D-GFP), to elucidate ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1186/s12929-015-0207-2
更新日期:2015-11-17 00:00:00