Abstract：
:Accumulative evidence has supported the role of nitric oxide (NO) in a variety of normal physiological functions as well as many pathological conditions. In this study, we examined the possible diabetogenicity of NO by measuring the expression of the insulin receptor substrate (IRS)-1 in rat hepatocytes and skeletal myocytes. IRS-1 is important in the insulin-mediated signal transduction pathway in both liver and skeletal muscle. Exogenous NO donated by S-nitroso-N-acetylpenicillamine (SNAP) and S-nitrosoglutathione (GSNO) resulted in significant reduction in levels of IRS-1 in both cells, when compared to the insulin-stimulated control (p<0.001). Reversal to near normal levels was achieved using the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (carboxy-PTIO). SNAP was the more potent drug, and the skeletal myocytes were the more sensitive cells to the inhibitory effects of NO released from the drugs. These results provide further evidence that exogenous NO is a potent modulator of insulin-mediated signal transduction and may play a significant role in the pathogenesis of type 2 diabetes mellitus.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Badal S,Brown PD,Ragoobirsingh Ddoi
10.1007/s11373-006-9073-ykeywords:
subject
Has Abstractpub_date
2006-07-01 00:00:00pages
561-8issue
4eissn
1021-7770issn
1423-0127journal_volume
13pub_type
杂志文章abstract：:Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy in terms of clinical features, underlying pathogenesis and treatment outcomes. Recent advances in genomic techniques have unraveled the molecular complexity of AML leukemogenesis, which in turn have led to refinement of risk stratification and pers...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-00674-7
更新日期：2020-07-21 00:00:00
abstract：:Reactive oxygen species (ROS) have been long considered simply as harmful by-products of metabolism, which damage cellular proteins, lipids, and nucleic acids. ROS are also known as a weapon of phagocytes, employed against pathogens invading the host. However, during the last decade, an understanding has emerged that ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-015-0194-3
更新日期：2015-10-15 00:00:00
abstract：:Retrograde transport of Wheat germ agglutinin conjugated to Horseradish peroxidase (WGA-HRP) was used in labeling vagal neurons projecting to the stomach from the dorsal motor nucleus of the vagus nerve (DMNV) in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in the experimental rats by intraperitone...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-006-9097-3
更新日期：2006-09-01 00:00:00
abstract：INTRODUCTION:Head and neck squamous carcinoma (HNSCC) is one of the most invasive types of cancer with high mortality. A previous study has indicated that low levels of let-7d and miR-205 in HNSCC patients are correlated with poor survival. Let-7d and miR-205 are tumor suppressors and regulators of epithelial-to-mesenc...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0511-3
更新日期：2019-02-13 00:00:00
abstract：BACKGROUND:Despite the recent progress in screening and therapy, a majority of prostate cancer cases eventually attain hormone refractory and chemo-resistant attributes. Conventional chemotherapeutic strategies are effective at very high doses for only palliative management of these prostate cancers. Therefore chemo-se...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0127-1
更新日期：2015-04-01 00:00:00
abstract：:The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransp...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-100
更新日期：2009-11-16 00:00:00
abstract：BACKGROUND:Iron homeostasis is chiefly regulated by hepcidin whose expression is tightly controlled by inflammation, iron stores, and hypoxia. Hemojuvelin (HJV) is a bone morphogenetic protein co-receptor that has been identified as a main upstream regulator of hepcidin expression; HJV mutations are associated with a s...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-83
更新日期：2012-09-21 00:00:00
abstract：BACKGROUND:The blood-spinal cord barrier (BSCB) is composed of a monolayer of endothelium linked with tight junctions and extracellular matrix (ECM)-rich basement membranes and is surrounded by astrocyte foot processes. Endothelial permeability is regulated by interaction between endothelial cells and ECM proteins. Fib...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0529-6
更新日期：2019-05-16 00:00:00
abstract：BACKGROUND:Previous study demonstrated the improvement of cardiac function was proportional to the number of cells implanted. Therefore, increasing cell survival in the infarcted myocardium might contribute to the improvement of the functional benefit of cell transplantation. METHODS AND RESULTS:MSCs were treated with...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9207-x
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Recent evidence suggests that drugs targeting Kv7 channels could be used to modulate vascular function and blood pressure. Here, we studied whether Kv7 channel inhibitors can be utilized to stabilize hemodynamics and reduce resuscitation fluid requirements after hemorrhagic shock. METHODS:Anesthetized male ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0316-1
更新日期：2017-01-17 00:00:00
abstract：:While virus-like particles (VLPs) containing subgenomic replicons, which can transduce replicons into target cells efficiently for studying viral replication and vectors of gene therapy and vaccine, have been established for several flaviviruses, none has been reported for the four serotypes of dengue virus, the causa...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9204-0
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Prevalence of metabolic syndrome (MS) is rapidly increasing worldwide. To investigate the spread of MS risks and its relationship with eating habits including fish intake, we carried out a health examination for young and middle-aged men. METHODS:The subjects were 97 healthy men (20 to 50 years) living in M...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-S1-S34
更新日期：2010-08-24 00:00:00
abstract：:The present study was undertaken to elucidate the mechanism of intra-arterial propofol-induced vascular permeability change resulting in tissue edema. The mechanism of propofol-induced hyperpermeability was examined in a rat femoral artery injection model. Vascular permeability was determined by measuring the Evans bl...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9164-4
更新日期：2007-09-01 00:00:00
abstract：:Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1007/s11373-005-9063-5
更新日期：2006-03-01 00:00:00
abstract：:An isolated thoracic spinal cord of the neonatal rat in vitro spontaneously generates sympathetic nerve discharge (SND) at ~25 degrees C, but it fails in SND genesis at < or = 10 degrees C. Basal levels of the c-Fos expression in the spinal cords incubated at < or = 10 degrees C and ~25 degrees C were compared to dete...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-44
更新日期：2009-05-01 00:00:00
abstract：:This study investigated the involvement of neutrophil infiltration, disturbances in nitric oxide (NO) generation and oxidative stress in indomethacin-induced gastric ulcer, and the possible gastroprotective potentials of leptin, known for its angiogenic effect. Male Wistar albino rats (180-220 g) were allocated into a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9227-6
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:Zerumbone, a sesquiterpene compound isolated from subtropical ginger, Zingiber zerumbet Smith, has been documented to exert antitumoral and anti- inflammatory activities. In this study, we demonstrate that zerumbone induces apoptosis in human glioblastoma multiforme (GBM8401) cells and investigate the apopto...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-19-86
更新日期：2012-10-05 00:00:00
abstract：:Zebrafish tbx5 expresses in the heart, pectoral fins and eyes of zebrafish during embryonic development. In zebrafish, injection of tbx5 morpholino antisense RNA caused changes of heart conformation, defect of heart looping, pericardium effusion, dropsy of ventral position and decreased heart rate. We suggested that c...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9268-5
更新日期：2008-11-01 00:00:00
abstract：:Oxidative stress and inflammation contributed to the propagation of acute liver injury (ALI). The present study was undertaken to determine whether D-galactosamine (D-GalN) induces ALI via the mitochondrial apoptosis- and proinflammatory cytokine-signaling pathways, and possible mechanism(s) by which green tea (GT) ex...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-35
更新日期：2009-03-25 00:00:00
abstract：:Stem cell activity is subject to non-cell-autonomous regulation from the local microenvironment, or niche. In adaption to varying physiological conditions and the ever-changing external environment, the stem cell niche has evolved with multifunctionality that enables stem cells to detect these changes and to communica...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-0624-8
更新日期：2020-03-14 00:00:00
abstract：:Alterations in eIF3-p48/INT6 gene expression have been implicated in murine and human mammary carcinogenesis. We examined levels of INT6 protein in human tumors and determined that breast and colon tumors clustered into distinct groups based on levels of INT6 expression and clinicopathological variables. We performed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9149-3
更新日期：2007-05-01 00:00:00
abstract：:The ARFP/F protein is synthesized from the +1 reading frame of the hepatitis C virus (HCV) core protein gene. The function of this protein remains unknown. To study the function of the HCV ARFP/F protein, we have conducted the yeast two-hybrid screening experiment to identify cellular proteins that may interact with t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9248-9
更新日期：2008-07-01 00:00:00
abstract：:Ischemia and reperfusion (I/R) - induced injury has been described as one of the main factors that contribute to the observed morbidity and mortality in a variety of clinical entities, including myocardial infarction, ischemic stroke, cardiac arrest and trauma. An imbalance between oxygen demand and supply, within the...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0220-0
更新日期：2016-01-19 00:00:00
abstract：:The SARS related Coronavirus genome contains a variety of novel accessory genes. One of these, called ORF7a or ORF8, code for a protein, known as 7a, U122 or X4. We set out to determine the three-dimensional structure of the soluble ectodomain of this type-I transmembrane protein by nuclear magnetic resonance spectros...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-9043-9
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:Sensing tissue acidosis is an important function of the somatosensory nervous system to response to noxious stimuli. MAIN BODY:In the pain clinic, acid or soreness sensation is a characteristic sensory phenotype of various acute and chronic pain syndromes, such as delayed onset muscle soreness, fibromyalgia...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0486-5
更新日期：2018-11-29 00:00:00
abstract：BACKGROUND:Mounting evidences have showed the important role of transforming growth factor-β (TGF-β) in immunological surveillance of tumors. Some studies have also indicated human leukocyte antigen (HLA)-G-associated immune escape involving TGF-β management in gastric cancer (GC). However, the mechanism underlying it ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0177-4
更新日期：2015-12-02 00:00:00
abstract：:Engineering approaches were adopted for liver microsystems to recapitulate cell arrangements and culture microenvironments in vivo for sensitive, high-throughput and biomimetic drug screening. This review introduces liver microsystems in vitro for drug hepatotoxicity, drug-drug interactions, metabolic function and enz...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-019-0575-0
更新日期：2019-10-28 00:00:00
abstract：BACKGROUND:Detection of cholangiocarcinoma (CCA) remains a diagnostic challenge. We established diagnostic peptide biomarkers in bile and urine based on capillary electrophoresis coupled to mass spectrometry (CE-MS) to detect both local and systemic changes during CCA progression. In a prospective cohort study we recen...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0599-5
更新日期：2020-01-03 00:00:00
abstract：:The signaling pathways leading to extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) activation by N-formyl-Met-Leu-Phe (fMLP) or platelet activating factor (PAF) in human neutrophils were examined. Previously, we found that changes of intracellular Ca2+ ([Ca2+]i) stimulated by...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-1704-1
更新日期：2005-01-01 00:00:00
abstract：:As a neurotropic virus, enterovirus A71 (EV-A71) emerge and remerge in the Asia-Pacific region since the 1990s, and has continuously been a threat to global public health, especially in children. Annually, EV-A71 results in hand-foot-and-mouth disease (HFMD) and occasionally causes severe neurological disease. Here we...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-019-0574-1
更新日期：2019-10-21 00:00:00
