Abstract：
:Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established. Despite having their own antioxidant system, peroxisomes undergo functional alterations during various conditions that are associated with free radical production such as inflammation, ischemia-reperfusion, carcinogenesis and diabetes. In this study we investigated the effect of diabetes on peroxisomal functions in rat kidneys and show for the first time that experimental diabetes induces redox-sensitive enhancement of peroxisomal activities. Streptozotocin-induced diabetes significantly increased (p < 0.01) beta-oxidation of lignoceric acid and the enzymic activity of acyl coenzyme A oxidase. Catalase activity was significantly reduced (p < 0.01) in the kidneys of diabetic rats, whereas the enzymic activity of DHAPATase (dihydroxyacetone phosphate acyltransferase) was not markedly affected by diabetes. Treatment of diabetic rats with antioxidants, thiocetic acid and vitamin C attenuated the diabetes-induced modulation of peroxisomal functions. The present study shows that the diabetes-induced effects on kidney peroxisomal functions are redox sensitive, and antioxidants might prove useful tools to alleviate nephropathy in diabetes.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Dhaunsi GS,Bitar MSdoi
10.1159/000079667keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
566-70issue
5eissn
1021-7770issn
1423-0127pii
79667journal_volume
11pub_type
杂志文章abstract：:Ubiquitination modulates a large repertoire of cellular functions and thus, dysregulation of the ubiquitin system results in multiple human diseases, including cancer. Ubiquitination requires an E3 ligase, which is responsible for substrate recognition and conferring specificity to ubiquitination. HUWE1 is a multiface...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-018-0470-0
更新日期：2018-09-04 00:00:00
abstract：:A number of infectious agents have been implicated in the development of vascular diseases such as atherosclerosis and posttransplantation arterial restenosis. Cytomegalovirus (CMV) has been reported to cause obliteration of coronary arteries by a progressive vasculopathy that involves proliferation of medial smooth m...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256111
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND:Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. METHODS:Induced keratinocyte-like cells (iKCs) were directly reprogrammed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00642-1
更新日期：2020-04-20 00:00:00
abstract：:Mediation of the respiratory reflex effects of an exogenous serotonin challenge goes beyond the lung vagi and is suggested to involve the nodose ganglia. In the present experiments the effects of an intravenous serotonin challenge on breathing pattern were studied in 8 pentobarbitone-chloralose anaesthetised cats. Bol...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256323
更新日期：2003-11-01 00:00:00
abstract：:Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256452
更新日期：2003-05-01 00:00:00
abstract：:Accumulative evidence has supported the role of nitric oxide (NO) in a variety of normal physiological functions as well as many pathological conditions. In this study, we examined the possible diabetogenicity of NO by measuring the expression of the insulin receptor substrate (IRS)-1 in rat hepatocytes and skeletal m...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-006-9073-y
更新日期：2006-07-01 00:00:00
abstract：:The present study was to investigate the effects and action mechanisms of dehydroepiandrosterone (DHEA) on steroidogenesis in rat adrenal zona glomerulosa cells (ZG). ZG cells were incubated with DHEA in the presence or absence of angiotensin II (AngII), a high concentration of potassium, 8-Br-cAMP, forskolin, 25-OH-c...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9241-3
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Dioscin, a typical steroid saponin, is isolated from Dioscorea nipponica Makino and Dioscorea zingiberensis Wright. It has estrogenic activity and many studies have also reported that dioscorea plants have an effect in preventing and treating osteoporosis. However, the molecular mechanisms underlying their e...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-30
更新日期：2014-04-17 00:00:00
abstract：:A large group of small Rab GTPases which mediate secretory and endosomal membrane transport, as well as autophagosome biogenesis, are essential components of vesicle trafficking machinery. Specific Rab protein together with the cognate effectors coordinates the dynamics of trafficking pathway and determines the cargo ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0287-7
更新日期：2016-10-06 00:00:00
abstract：BACKGROUND:Endothelial cell (EC) dysfunctions, including turnover enrichment, gap junction disruption, inflammation, and oxidation, play vital roles in the initiation of vascular disorders and atherosclerosis. Hemodynamic forces, i.e., atherprotective pulsatile (PS) and pro-atherogenic oscillatory shear stress (OS), ca...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-019-0551-8
更新日期：2019-08-07 00:00:00
abstract：:Human mesenchymal stem cells (MSCs) are multilineage somatic progenitor/stem cells that have been shown to possess immunomodulatory properties in recent years. Initially met with much skepticism, MSC immunomodulation has now been well reproduced across tissue sources and species to be clinically relevant. This has ope...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-016-0289-5
更新日期：2016-11-04 00:00:00
abstract：:The aim of this study was to determine whether the common forms of dyslipidemia could affect either the lipid composition or insulin receptor processing (down-regulation) of erythrocytes. The study included 22 patients with type IIa hypercholesterolemia, 15 patients with type IV hypertriglyceridemia and 12 patients wi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253384
更新日期：1995-08-01 00:00:00
abstract：:Permanent functional deficit in patients with spinal cord injury (SCI) is in part due to severe neural cell death. Therefore, cell replacement using stem cells and neural progenitors that give rise to neurons and glia is thought to be a potent strategy to promote tissue repair after SCI. Many studies have shown that s...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256573
更新日期：2002-01-01 00:00:00
abstract：:Chronic inflammation may lead to endothelial dysfunction, which manifests as an impaired coronary reactivity. Impairment in coronary flow reserve (CFR), preceding the clinical symptoms of coronary artery disease, can be measured noninvasively by positron emission tomography. Myeloperoxidase (MPO) is an oxidative enzym...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256549
更新日期：2004-01-01 00:00:00
abstract：:The Peutz-Jeghers syndrome (PJS) is a hereditary disorder that predisposes an individual to benign and malignant tumors in multiple organ systems. Recently, the locus responsible for PJS was mapped genetically to the LKB1 gene, with a subsequent investigation proving that it is responsible for most cases of PJS. LKB1 ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256059
更新日期：2003-03-01 00:00:00
abstract：:Myostatin, a member of the TGF-beta superfamily, is a potent negative regulator of skeletal muscle and growth. Previously, we reported Mstn1 from zebrafish and studied its influence on muscle development. In this study, we identified another form of Myostatin protein which is referred to as Mstn2. The size of Mstn2 cD...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9250-2
更新日期：2008-09-01 00:00:00
abstract：:Schistosomiasis causes significant levels of morbidity and mortality in many geographical regions of the world. The disease is caused by infections with parasitic blood flukes known as schistosomes. The control of schistosomiasis over the last several decades has been centered on the mass drug administration (MDA) of ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-020-0621-y
更新日期：2020-01-22 00:00:00
abstract：:Drosophila eye development is a progressive process including cell fate determination, pattern formation, and rhabdomere morphogenesis. During eye development, a dramatic change in cell shape, which involves turning and extension of the photoreceptor apical surface, occurs in the early pupal stages. It is known that a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02254441
更新日期：2004-05-01 00:00:00
abstract：:Upon cell adhesion to extracellular matrix proteins, focal adhesion kinase (FAK) rapidly undergoes autophosphorylation on its Tyr-397 which consequently serves as a binding site for the Src homology 2 domains of the Src family protein kinases and several other intracellular signaling molecules. In this study, we have ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-005-7212-5
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus, it is of great interest to look for an...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-18-42
更新日期：2011-06-17 00:00:00
abstract：BACKGROUND:Promoting post-stroke neurogenesis has long been proposed to be a therapeutic strategy for the enhancement of functional recovery after cerebral ischemic stroke. Despite numerous approaches have been widely reported the proliferation or differentiation of the neurogenic population therapeutic strategies by t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0521-1
更新日期：2019-04-18 00:00:00
abstract：:The detailed mechanism of how lysophosphatidylcholine (LPC) suppresses endothelium-dependent vasodilatation is unclear at present. We investigated the effects of LPC on endothelial intracellular calcium (EC [Ca(2+)](i)) signaling and vascular tone simultaneously using a new technique we developed. Fura-2-labeled rat a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256588
更新日期：2002-07-01 00:00:00
abstract：:The likelihood that expression of a foreign gene in a mammalian cell is deleterious to viability is confronted whenever novel transgenic animals are made. A pathological response to transgene expression is even desired in transgenic mouse models of human disease. The derivation of HIV-transgenic mice in our laboratory...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253065
更新日期：1995-04-01 00:00:00
abstract：BACKGROUND:Asthma has become an important public health issue and approximately 300 million people have suffered from the disease worldwide. Nowadays, the use of acupuncture in asthma is increasing. This study intended to systematically analyze and compare the gene expression profiles between the asthmatic and acupunct...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-46
更新日期：2009-05-06 00:00:00
abstract：BACKGROUND:Glioma stem-like cells (GSCs) are proposed to be responsible for high resistance in glioblastoma multiforme (GBM) treatment. In order to find new strategies aimed at reducing GSC stemness and improving GBM patient survival, we investigated the effects and mechanism of a histone deacetylases (HDACs) inhibitor...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0296-6
更新日期：2016-11-18 00:00:00
abstract：BACKGROUND:Dengue virus (DENV), a member of the family Flaviviridae, is at present the most widespread causative agent of a human viral disease transmitted by mosquitoes. Despite the increasing incidence of this pathogen, there are no antiviral drugs or vaccines currently available for treatment or prevention. In a pre...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0134-2
更新日期：2015-04-17 00:00:00
abstract：:Although hypercholesterolemia is a well-known risk factor for atherosclerosis, little is known about the effect of hypercholesterolemia on cardiac contractile function. The objective of this study was to examine the effect of hypercholesterolemia on myocardial contractility. Fifteen New Zealand white rabbits were fed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02254368
更新日期：2004-11-01 00:00:00
abstract：BACKGROUND:Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine and elevated in the regions of tissue injury and inflammatory diseases. The deleterious effects of TNF-α on fibroblasts may aggravate heart inflammation mediated through the up-regulation of adhesion molecules such as vascular cell adhesion molecu...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0165-8
更新日期：2015-07-15 00:00:00
abstract：:Sequencing of HIV-1(GER) long terminal repeat (LTR) has demonstrated, for the first time in an HIV-1 primary isolate, a TAR duplication referred to as TAR1 (nucleotides +1 through +68) and TAR2 (nucleotides +69 through +136). This TAR duplication is stable during replication of HIV-1(GER) isolate in CEM cells. Analysi...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253576
更新日期：1996-01-01 00:00:00
abstract：:Fibroblasts derived from glucose-6-phosphate dehydrogenase (G6PD)-deficient patients display retarded growth and accelerated cellular senescence that is attributable to increased accumulation of oxidative DNA damage and increased sensitivity to oxidant-induced senescence, but not to accelerated telomere attrition. Her...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-18
更新日期：2009-02-05 00:00:00
