The Giemsa stain: its history and applications.

Abstract:

:Gustav Giemsa was born in Germany in 1867, worked mainly as a chemist, and died in 1948. The staining method, which carries his name, was designed primarily for the demonstration of parasites in malaria, but it was also employed in histology because of the high-quality staining of the chromatin and the nuclear membrane, the metachromasia of some cellular components, and the different qualities of cytoplasmic staining depending on the cell type. The use of methylene azure and its mixture with methylene blue to form an eosinate made stable the stain and its results. Giemsa's stain is regarded as the world's standard diagnostic technique for malaria's plasmodium, and it is also the basic stain for classifying lymphomas in the Kiel classification.

journal_name

Int J Surg Pathol

authors

Barcia JJ

doi

10.1177/1066896907302239

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

292-6

issue

3

eissn

1066-8969

issn

1940-2465

pii

15/3/292

journal_volume

15

pub_type

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