Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity.

abstract：:RNA polymerase pausing represents an important mechanism of transcriptional regulation. In this study, we use a single-molecule transcription assay to investigate the effect of template base-pair composition on pausing by RNA polymerase II and the evolutionarily distinct mitochondrial polymerase Rpo41. For both enzyme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamft B,Bintu L,Ishibashi T,Bustamante C

更新日期：2012-06-05 00:00:00

abstract：:A nongenetic, transgenerational effect of parental age on offspring fitness has been described in many taxa in the laboratory. Such a transgenerational fitness effect will have important influences on population dynamics, population age structure, and the evolution of aging and lifespan. However, effects of parental a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schroeder J,Nakagawa S,Rees M,Mannarelli ME,Burke T

更新日期：2015-03-31 00:00:00

abstract：:Mutations in the human glucocerebrosidase gene cause Gaucher disease. A cDNA clone containing the entire human glucocerebrosidase coding region from normal cells has been isolated using lambda gt11 expression libraries. The complete nucleotide sequence, a restriction map, and a hydropathy profile are presented. Hybrid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sorge J,West C,Westwood B,Beutler E

更新日期：1985-11-01 00:00:00

abstract：:The time-dependent Schrödinger equation is a cornerstone of quantum physics and governs all phenomena of the microscopic world. However, despite its importance, its origin is still not widely appreciated and properly understood. We obtain the Schrödinger equation from a mathematical identity by a slight generalization...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schleich WP,Greenberger DM,Kobe DH,Scully MO

更新日期：2013-04-02 00:00:00

abstract：:Maximum likelihood analysis for linkage between autosomal dominant aniridia and 12 biochemical and serological markers in a single large family showed a probable linkage between autosomal dominant aniridia and the enzyme acid phosphatase-1. The presence of an autosomal dominant aniridia gene linked to acid phosphatase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrell RE,Chakravarti A,Hittner HM,Riccardi VM

更新日期：1980-03-01 00:00:00

abstract：:The localized maternal RNA Vg1 resides in the cortical region of the vegetal pole of fully grown Xenopus oocytes and is inherited by only a subset of blastomeres in the early embryo [Weeks, D. L. & Melton, D. A. (1987) Cell 51, 861-867]. Because RNA-cytoskeletal interactions may play a role in RNA localization, we hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pondel MD,King ML

更新日期：1988-10-01 00:00:00

abstract：:Soluble, glutathione-stimulated delta 5-3-ketosteroid isomerase (EC 5.3.3.A) activity of human and rat liver resides in very basic proteins with molecular weights of about 45,000 which are present in high concentrations in these tissues. Physiochemical and immunological evidence is presented for the identity of the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benson AM,Talalay P,Keen JH,Jakoby WB

更新日期：1977-01-01 00:00:00

abstract：:The N-terminal arginine-rich motif of phage HK022 Nun protein binds to NUT sequences in phage lambda nascent transcripts and induces transcription termination. Interactions between the Nun C terminus and RNA polymerase as well as the DNA template are required for termination. We have isolated Nun C-terminal point and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HC,Zhou JG,Wilson HR,Mogilnitskiy G,Court DL,Gottesman ME

更新日期：2003-04-29 00:00:00

abstract：:Climate change is expected to cause shifts in the composition of tropical montane forests towards increased relative abundances of species whose ranges were previously centered at lower, hotter elevations. To investigate this process of "thermophilization," we analyzed patterns of compositional change over the last de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duque A,Stevenson PR,Feeley KJ

更新日期：2015-08-25 00:00:00

abstract：:Picosecond studies of rhodopsin in low-temperature glasses have been carried out in order to observe directly the risetime of prelumirhodopsin, the first intermediate in the visual pathway. Only at 20 K or below can the risetime of this intermediate be resolved and even at 4 K it is astoundingly rapid, about 36 psec. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peters K,Applebury ML,Rentzepis PM

更新日期：1977-08-01 00:00:00

abstract：:Established T cell dysfunction is a barrier to antitumor responses, and checkpoint blockade presumably reverses this. Many patients fail to respond to treatment and/or develop autoimmune adverse events. The underlying reason for T cell responsiveness remains elusive. Here, we show that susceptibility to checkpoint blo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelson CE,Mills LJ,McCurtain JL,Thompson EA,Seelig DM,Bhela S,Quarnstrom CF,Fife BT,Vezys V

更新日期：2019-02-12 00:00:00

abstract：:Major histocompatibility complex (MHC) class I multimer technology has become an indispensable immunological assay system to dissect antigen-specific cytotoxic CD8(+) T cell responses by flow cytometry. However, the development of high-throughput assay systems, in which T cell responses against a multitude of epitopes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bakker AH,Hoppes R,Linnemann C,Toebes M,Rodenko B,Berkers CR,Hadrup SR,van Esch WJ,Heemskerk MH,Ovaa H,Schumacher TN

更新日期：2008-03-11 00:00:00

abstract：:Although most nuclear hormone receptors are ligand-dependent transcriptional activators, certain members of this superfamily, such as thyroid hormone receptor (TR) and retinoic acid receptor (RAR), are involved in transcriptional repression. The silencing function of these receptors has been localized to the ligand bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nawaz Z,Tsai MJ,O'Malley BW

更新日期：1995-12-05 00:00:00

abstract：:Cotransfection assays were used to show that the members of the GTP-binding protein Gq class of alpha subunits could activate phospholipase C (PLC) beta 2. Similar experiments also demonstrated that G beta 1 gamma 1, G beta 1 gamma 5, and G beta 2 gamma 5 could activate the beta 2 isoform of PLC but not the beta 1 iso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu D,Katz A,Simon MI

更新日期：1993-06-01 00:00:00

abstract：:The cheA locus of Escherichia coli, which is required for chemotactic behavior, encodes two polypeptide products designated p[cheA]L and p[cheA]S. The mode of synthesis of these two proteins was investigated by transferring various missense and nonsense mutations to a lambda transducing phage and observing the mutant ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith RA,Parkinson JS

更新日期：1980-09-01 00:00:00

abstract：:A duplex 21 nucleotide small interfering RNA (siRNA) against human Keap1 is described that represents a unique class of cancer chemopreventive agent. This siRNA can knockdown Keap1 mRNA and thereby relieve negative regulation of nuclear factor erythroid 2 p45-related factor 2 (Nrf2)-mediated gene expression. The siRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Devling TW,Lindsay CD,McLellan LI,McMahon M,Hayes JD

更新日期：2005-05-17 00:00:00

abstract：:Carbon nanotube (CNT) membranes were employed as the active element of a switchable transdermal drug delivery device that can facilitate more effective treatments of drug abuse and addiction. Due to the dramatically fast flow through CNT cores, high charge density, and small pore dimensions, highly efficient electroph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu J,Paudel KS,Strasinger C,Hammell D,Stinchcomb AL,Hinds BJ

更新日期：2010-06-29 00:00:00

abstract：:We present a theory, and its computer implementation, of how mental simulations underlie the abductions of informal algorithms and deductions from these algorithms. Three experiments tested the theory's predictions, using an environment of a single railway track and a siding. This environment is akin to a universal Tu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khemlani SS,Mackiewicz R,Bucciarelli M,Johnson-Laird PN

更新日期：2013-10-15 00:00:00

abstract：:Stem cell therapy has emerged as a promising tool for the treatment of a variety of diseases. Previously, we have shown that Akt-modified mesenchymal stem cells mediate tissue repair through paracrine mechanisms. Using a comprehensive functional genomic strategy, we show that secreted frizzled related protein 2 (Sfrp2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mirotsou M,Zhang Z,Deb A,Zhang L,Gnecchi M,Noiseux N,Mu H,Pachori A,Dzau V

更新日期：2007-01-30 00:00:00

abstract：:This report describes the integration of laser-scanning fluorometric cytometry and nonseparation ligand-binding techniques to provide new assay methods adaptable to miniaturization and high-throughput screening. Receptor-bound, cyanine dye-labeled ligands, [Cy]ligands, were discriminated from those free in solution by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuck P,Lao Z,Skwish S,Glickman JF,Yang K,Burbaum J,Inglese J

更新日期：1999-09-28 00:00:00

abstract：:Complement receptors (CRs), expressed notably on myeloid and lymphoid cells, play an essential function in the elimination of complement-opsonized pathogens and apoptotic/necrotic cells. In addition, these receptors are crucial for the cross-talk between the innate and adaptive branches of the immune system. CR3 (also...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bajic G,Yatime L,Sim RB,Vorup-Jensen T,Andersen GR

更新日期：2013-10-08 00:00:00

abstract：:Understanding protein-protein interactions is central to our understanding of almost all complex biological processes. Computational tools exploiting rapidly growing genomic databases to characterize protein-protein interactions are urgently needed. Such methods should connect multiple scales from evolutionary conserv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gueudré T,Baldassi C,Zamparo M,Weigt M,Pagnani A

更新日期：2016-10-25 00:00:00

abstract：:Embryonal kidney cell tumors develop in rats given the alkylating agent N-nitroso-N'-methylurea as neonates. These tumors resemble the childhood Wilms tumors in their histopathology. Deletions and mutations in the Wilms tumor suppressor gene, WT1, are present in up to 6% of childhood nephroblastomas. To investigate th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma PM,Bowman M,Yu BF,Sukumar S

更新日期：1994-10-11 00:00:00

abstract：:Modified bovine leukemia virus (BLV) glycoproteins were expressed by using vaccinia virus recombinants, and their fusogenic capacities were examined by a syncytia-formation assay. This analysis indicates that (i) both BLV envelope glycoproteins gp51 and gp30 are necessary for cell fusion; (ii) insertion of the N-termi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vonèche V,Portetelle D,Kettmann R,Willems L,Limbach K,Paoletti E,Ruysschaert JM,Burny A,Brasseur R

更新日期：1992-05-01 00:00:00

abstract：:The ability of cocaine to inhibit the dopamine transporter (DAT) appears to be crucial for its reinforcing properties. The potential use of drugs that produce long-lasting inhibition of the DAT as a mean of preventing the "high" and reducing drug-seeking behavior has become a major strategy in medication development. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volkow ND,Wang GJ,Fowler JS,Gatley SJ,Ding YS,Logan J,Dewey SL,Hitzemann R,Lieberman J

更新日期：1996-09-17 00:00:00

abstract：:The crystal structure of raite was solved and refined from data collected at Beamline Insertion Device 13 at the European Synchrotron Radiation Facility, using a 3 x 3 x 65 microm single crystal. The refined lattice constants of the monoclinic unit cell are a = 15.1(1) A; b = 17.6(1) A; c = 5.290(4) A; beta = 100.5(2)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pluth JJ,Smith JV,Pushcharovsky DY,Semenov EI,Bram A,Riekel C,Weber HP,Broach RW

更新日期：1997-11-11 00:00:00

abstract：:Escherichia coli glutamine synthetase (GS) was inactivated by a nonenzymic mixed-function oxidation system composed of ascorbate, O2, and Fe(III). Partial inactivation of GS by this system leads to the formation of hybrid GS molecules (dodecamers) composed of both active and inactive subunits. Subunit interactions in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura K,Stadtman ER

更新日期：1984-04-01 00:00:00

abstract：:We recently showed that culture-derived metacyclic trypomastigotes (CMT), but not epimastigotes (Epi), of the Miranda 88 strain of Trypanosoma cruzi evade lysis by the human alternative complement pathway because of inefficient binding of factor B to complement component C3b on the parasite surface. These results sugg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rimoldi MT,Sher A,Heiny S,Lituchy A,Hammer CH,Joiner K

更新日期：1988-01-01 00:00:00

abstract：:The last 2 decades have seen discoveries in highly excited states of atoms and molecules of phenomena that are qualitatively different from the "planetary" model of the atom, and the near-rigid model of molecules, characteristic of these systems in their low-energy states. A unified view is emerging in terms of approx...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kellman ME

更新日期：1996-12-10 00:00:00

abstract：:Two photochromic activators of the electrogenic membrane of the electroplax of Electrophorus electricus are described. Trans-3,3'-bis[alpha-(trimethylammonium)methyl]azobenzene dibromide (Bis-Q), one of the most potent ever reported, is active at concentrations of less than 10(-7) M. Its cis isomer, which is obtained ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartels E,Wassermann NH,Erlanger BF

更新日期：1971-08-01 00:00:00