Abstract：
:DNA distribution in mouse, rabbit and bull spermatids was analyzed by electron microscopy, after using a Feulgen-like HCl-osmium ammine procedure, and after immunocytochemistry with anti-DNA antibodies. In addition, nucleic acids were visualized with the intercalating dye ethidium bromide and phosphotungstic acid. The parts of DNA displaying a beta helix configuration (possibly A-T rich parts) were identified by epifluorescence microscopy after staining with Hoechst 33258. In all 3 species, young spermatid nuclei were seen to have large areas poor in DNA, as well as DNA-rich areas, which were mostly concentrated into a peripheral layer close to the acrosome and into one or several masses, displaying species-specific locations. These DNA-rich areas were stained with Hoechst 33258. Elongating spermatid nucleic contained homogeneously distributed DNA, and this was evident following both immunocytochemistry and nucleic acid histochemistry in all 3 species. However, the distribution appeared more heterogeneous after the Feulgen-like procedure, and was accompanied by a disappearance of Hoechst-fluorescence. In fully elongated spermatids, all nuclear areas stained with Hoechst 33258, while the 3 other techniques labeled either all or species-specific parts of the condensed chromatin. The reasons for these variable reactions are discussed in terms of technique specificities, DNA configuration and nucleoprotein moiety replacements.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Courtens JL,Biggiogera M,Fakan Sdoi
10.1007/BF00340875subject
Has Abstractpub_date
1991-09-01 00:00:00pages
517-25issue
3eissn
0302-766Xissn
1432-0878journal_volume
265pub_type
杂志文章abstract：:Apart from the known hypothalamic controls, which have been well documented, a myriad of compounds both endogenous and exogenous have proved effective in influencing secretion of prolactin (PRL). Recent studies have shown that somatostatin (SRIF), when injected intra-atrially as a bolus, is able to inhibit PRL secreti...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222371
更新日期：1983-01-01 00:00:00
abstract：:The neurotensin-cell is identified immunohistochemically and ultrastructurally by differential counting of endocrine cells in the gut of a primate (Tupaia belangeri). Utilizing light microscopy, the EC-cells are identified by the Masson-Fontana silver stain; with the same method the neurotensin cells are not stained. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220968
更新日期：1977-11-23 00:00:00
abstract：:The presence of melatonin is demonstrated in the pineal gland, the retina and the Harderian gland in some mammalian and non-mammalian vertebrates, using a specific fluorescence labelled antibody technique. Four different potent antibodies against melatonin have been used and compared. In the pineal gland of hamsters, ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233830
更新日期：1981-01-01 00:00:00
abstract：:In situ hybridisation and immunohistochemistry analyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFalpha), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-010-0993-5
更新日期：2010-08-01 00:00:00
abstract：:The ultrastructure and distribution of adherens junctions in the intact adult lens of human, chicken, dove, rat, and rainbow trout were studied with thin-section electron microscopy, using an improved fixation containing a mixture of glutaraldehyde, lysine, and tannic acid. The nature of adherens junctions in the fibe...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00220014
更新日期：1988-10-01 00:00:00
abstract：:In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer'...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318501
更新日期：1995-02-01 00:00:00
abstract：:The therapeutic potency of delivered mesenchymal stem cells (MSCs) in tissue engineering applications may be improved by priming cells toward a differentiated state via coculture with native, differentiated cells prior to implantation; however, there is a lack of understanding in what may be the most efficacious metho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1265-8
更新日期：2012-03-01 00:00:00
abstract：:The supraependymal cells (SEC) are a normal component of the wall of the cerebral ventricles. In the hypothalamic area of the third ventricle they are restricted, in healthy animals, to the ependymal projection of the hypophyseotropic area. Here the SEC show great polymorphism. In addition to bipolar, multipolar and s...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215308
更新日期：1976-05-13 00:00:00
abstract：:The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0667-8
更新日期：2008-10-01 00:00:00
abstract：:The medial preoptic nucleus is a sexually dimorphic structure whose cytoarchitecture, afferent and efferent connections, and functions have been previously described. No detailed ultrastructural study has, however, been performed to date. Here we describe the ultrastructural organization of this important preoptic str...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00318164
更新日期：1995-03-01 00:00:00
abstract：:The gross structure as well as the neuronal and non-neuronal components of the lamina ganglionaris of the locust Schistocerca gregaria are described on the basis of light- and electron-microscopical preparations of Golgi (selective silver) and ordinary histological preparations. The array of optic cartridges within th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233626
更新日期：1981-01-01 00:00:00
abstract：:Scanning electron microscopy was used to investigate the morphological changes of the tail musculature of the metamorphosing anuran tadpole, attention being focused on phagocytosis by macrophages. Muscle fibers were stained en bloc with silver and freeze-fractured during dehydration, or torn after drying. Samples were...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214574
更新日期：1985-01-01 00:00:00
abstract：:Two types of mitochondria-rich cells were found in the interplatelet areas of the gills of the migrating river lamprey. Both cell types are thought to be responsible for ion-transport across the gills. In the fresh-run migrant the gills are dominated by large, flask-shaped cells which show some ultrastructural similar...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221380
更新日期：1976-10-06 00:00:00
abstract：:Considerable evidence indicates that the state of ocular connective tissues and their response in glaucomatous disease affect the degree of glaucoma damage. Both experimental and clinical data suggest that improved diagnostic and prognostic information can be derived from the assessment of the mechanical responsivenes...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-013-1603-0
更新日期：2013-08-01 00:00:00
abstract：:The fine structure of luminal surface of clearly identified portions of uriniferous tubules has been studied by scanning electron microscopy to elucidate some controversies concerning the topography of certain surface formations. The results show a characteristic pattern of the luminal surface in the region of Henle's...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215128
更新日期：1976-02-06 00:00:00
abstract：:Distribution of microtubules and other cytoskeletal filaments in growing skeletal muscle cells (myotubes) was studied in vitro by fluorescence microscopy using fluorescin-labeled antibodies and phalloidin, a specific antiactin drug. In the distal elongating tips of myotubes, microtubules were the major cytoskeletal el...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00214368
更新日期：1988-05-01 00:00:00
abstract：:The stem cells with their distinct ability of self-renewal and differentiation are considered an innovation in wound healing. However, there is lack of studies comparing the differential effect of the type and administration route of stem cells in the wound healing context. Thus, the current study has been designed to...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-018-2879-x
更新日期：2018-11-01 00:00:00
abstract：:The distribution of luteinizing hormone-releasing hormone (LHRH) was studied in the rat and mouse brain by means of light and electron microscopic immunohistochemistry using the peroxidase-antiperoxidase method. An immunoreactive product to LHRH antiserum was found near the blood vessels of the vascular organ of the l...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233914
更新日期：1979-03-19 00:00:00
abstract：:Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00583405
更新日期：1995-08-01 00:00:00
abstract：:The fan-shaped body is the largest substructure of the central complex in Drosophila melanogaster. Two groups of large-field neurons that innervate the fan-shaped body, viz., F1 and F5 neurons, have recently been found to be involved in visual pattern memory for "contour orientation" and "elevation" in a rut-dependent...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-009-0781-2
更新日期：2009-06-01 00:00:00
abstract：:Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (P...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-012-1446-0
更新日期：2012-10-01 00:00:00
abstract：:Changes in the organisation and composition of extracellular matrix in human endometrium during the menstrual cycle and early pregnancy have been assessed by immunofluorescence. Amongst interstitial components, type-III and type V-collagens and fibronectin are present in endometrial stroma throughout the menstrual cyc...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221758
更新日期：1988-07-01 00:00:00
abstract：:The three-dimensional structure of the mitochondria and sarcoplasmic reticulum (SR) in the three types of twitch fibers, i.e., the red, white and intermediate skeletal muscle fibers, of the vastus lateralis muscle of the Japanese meadow frog (Rana nigromaculata nigromaculata Hallowell) was examined by high resolution ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218939
更新日期：1987-12-01 00:00:00
abstract：:The azygos vein of the rat can be divided into three regions: The proximal cardiac region, where the wall is composed of two and sometimes three layers of cardiac muscle and a thin discontinuous layer of smooth muscle cells. Vascular casts of this region demonstrate layers of capillaries closely following the orientat...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221867
更新日期：1986-01-01 00:00:00
abstract：:An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00218363
更新日期：1982-01-01 00:00:00
abstract：:Synapses in the lateral septum of the murine brain have been investigated by ultrastructural immunocytochemistry, using monoclonal anti-neurophysins in both immunoperoxidase and immunogold techniques. In the region shown by light microscopy to be rich in vasopressinergic innervation, synaptic boutons containing approx...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215524
更新日期：1987-08-01 00:00:00
abstract：:The dorsal integument of the girdle of the chiton Mopalia muscosa is covered by a chitinous cuticle about 0.1 mm in thickness. Within the cuticle are fusiform spicules composed of a central mass of pigment granules surrounded by a layer of calcium carbonate crystals. Tapered, curved chitinous hairs with a groove on th...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00221498
更新日期：1982-01-01 00:00:00
abstract：:The adenohypophysial primordium of Xenopus laevis tadpoles at stages 33/34 to 46 (Nieuwkoop and Faber, 1956) were examined immunohistologically for alpha-MSH, beta-MSH and ACTH. beta-MSH was demonstrated from stage 37/38 onwards, and alpha-MSH from stage 39. No signs of ACTH production were detected. alpha-MSH and bet...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00231955
更新日期：1977-05-16 00:00:00
abstract：:Placenta-derived stem cells (PDSCs) have gained interest as an alternative source of stem cells for regenerative medicine because of their potential for self-renewal and differentiation and their immunomodulatory properties. Although many studies have characterized various PDSCs biologically, the properties of the sel...
journal_title：Cell and tissue research
pub_type： 临床试验,杂志文章
doi：10.1007/s00441-011-1249-8
更新日期：2011-10-01 00:00:00
abstract：:Extracellular matrix (ECM) plays a dynamic role during tissue development and re-growth. Body part regeneration efficiency relies also on effective ECM remodelling and deposition. Among invertebrates, echinoderms are well known for their striking regenerative abilities since they can rapidly regenerate functioning com...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03201-0
更新日期：2020-09-01 00:00:00
