Abstract:
:The stem cells with their distinct ability of self-renewal and differentiation are considered an innovation in wound healing. However, there is lack of studies comparing the differential effect of the type and administration route of stem cells in the wound healing context. Thus, the current study has been designed to elucidate the effect of two of the most important stem cell types-the bone marrow-derived and adipose-derived stem cells in full thickness wound healing-and to evaluate, in this optimized wound model, the effectiveness of intradermal versus intravenous routes using H&E, Masson's trichrome, and PKH26-stained sections. It also evaluated the immunohistochemical expression of the stem cell-related surface markers-Ki67, CD71, CD146, CD90, and CD163-and also assessed the level of TNFα and gene expression of NF-κB as two important inflammatory markers. The study revealed that the adipose stem cell groups have shown statistically significant improvement in inflammation, granulation tissue re-organization, and collagen deposition relative to their bone marrow-treated counterparts. The intradermally treated adipose stem cell group, in particular, has demonstrated the most supreme features regarding the expression of the proliferation-related surface markers Ki67 and CD71 as well as in the expression of CD90 in keratinocytes and hair follicle dermal sheaths. The same group has shown the lowest level of TNFα and the best outcome in the parameters of neo-epidermal thickness, granulation tissue re-organization, and pattern of collagen deposition. The systemically treated wounds have displayed superior expression of CD146-positive endothelial cells and dermal fibroblasts as well as better expression of CD163+ macrophages.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Aboulhoda BE,Abd El Fattah Sdoi
10.1007/s00441-018-2879-xsubject
Has Abstractpub_date
2018-11-01 00:00:00pages
285-302issue
2eissn
0302-766Xissn
1432-0878pii
10.1007/s00441-018-2879-xjournal_volume
374pub_type
杂志文章abstract::The distribution of Mg++-ATPase in osteoclasts along the endosteal surface of the chick tibia was investigated by neutral and alkaline pH cytochemical methods at the electron-microscopic level. Reaction product was observed in mitochondria, cytoplasmic vesicles, and ruffled-border membrane. Levamisole, ouabain, and va...
journal_title:Cell and tissue research
pub_type: 杂志文章
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abstract::The effects of cytochalasin B (CB) on pancreatic structure and amylase release were studied by use of pancreatic fragments, isolated acini and isolated acinar cells. In pancreatic fragments and isolated acini CB caused the disappearance of microfilaments underlying the apical plasma membrane, loss of apical microvilli...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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abstract::Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (P...
journal_title:Cell and tissue research
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abstract::In vitro incubation of autonomic nerves with vinblastine sulphate (1 times 10(-4) M) caused a disappearance of microtubules within 15 min; during the following 15 min paracrystalline arrays appeared within the axons. An increase in the abundance of microfilaments was also observed, but these did not appear to arise fr...
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abstract::The mechanism of cell death which occurs during Chagas' cardiopathy is disputed. To address this issue we analyzed the molecular pathways implicated in the death of cardiomyocytes during T. cruzi invasion and found that they undergo apoptosis during both in vitro and in vivo infections. However, the death rates and on...
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journal_title:Cell and tissue research
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abstract::The antigen detected by the rat anti-mouse monoclonal antibody (m Ab), anti-BSP-3, has been initially described as a brain cell-surface protein. Evidence is presented that this m Ab recognizes mouse (Na+ + K+)-ATPase (ATP phosphohydrolase, E.C.3.6.1.3). The antigen, purified from mouse brain by means of affinity chrom...
journal_title:Cell and tissue research
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journal_title:Cell and tissue research
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-013-1618-6
更新日期:2013-06-01 00:00:00
abstract::Thyroid glands of 7 to 21 day-old chick embryos were examined by electron microscopy using freeze-fracture and thin-section preparations. The primitive follicle lumen first appears between two adjacent epithelial cells in 8 day-old embryos, and is formed in the region of a focal tight junction (macula occludens). The ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00234830
更新日期:1979-04-30 00:00:00
abstract::Osteoarthritis (OA) is the most common joint disease with an unsatisfactory therapy outcome and characterized by the degradation of articular cartilage and synovial inflammation. Here, we isolated bone marrow mesenchymal stem cells (BMSCs) from rat's bone marrow and BMSC-derived exosome (BMSCs-Exo) from BMSCs successf...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-020-03319-1
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abstract::The skeletal body muscle of vertebrates is derived from segmentally arranged mesodermal structures, the somites. Only the dorsal epithelial half of the somite, the dermomyotome, gives rise to muscle cells during normal development. Head muscle takes its origin from the somites, the unsegmented paraxial head mesoderm a...
journal_title:Cell and tissue research
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更新日期:1999-04-01 00:00:00
abstract::The vascularization of the hypothalamo-adenohypophysial region of the toad Bufo bufo (L.) (Amphibia, Anura) was studied by means of light- and scanning electron microscopy. Special attention was given to the portal vascular system of the median eminence and the pars distalis. Course and arrangement of these vessels ar...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00223268
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00239453
更新日期:1989-11-01 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-005-1118-4
更新日期:2005-08-01 00:00:00
abstract::Glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (SCO) and the pineal organ of the sheep (Ovis aries). By use of concanavalin A labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the SCO. In the pineal organ, e...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00218562
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pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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abstract::In order to compare the functional state of the anterior pituitary of spontaneously hypertensive rats (SHR) with that of nonmotensive Wistar Kyoto rats (WKR), the anterior pituitary was examined by morphometry and autoradiography at the level of electron microscopy. The relative number and the relative volume of thyro...
journal_title:Cell and tissue research
pub_type: 杂志文章
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abstract::Previous neurohistological studies have been extended to include the structures contained solely or mainly within the junctional esophageal segment which may play an important role in the sphincter mechanism. The main findings were: 1) a progressive cranio-caudal thickening of the muscularis mucosae; 2) a conspicuous ...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00215998
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abstract::Kisspeptin is a hypothalamic neuropeptide, which acts directly on gonadotropin-releasing hormone (GnRH)-secreting neurons via its cognate receptor (GPR54 or Kiss-R) to stimulate GnRH secretion in mammals. In non-mammalian vertebrates, there are multiple kisspeptins (Kiss1 and Kiss2) and Kiss-R types. Recent gene knock...
journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/s00441-019-03089-5
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00333704
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00583405
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00221791
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
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journal_title:Cell and tissue research
pub_type: 杂志文章
doi:10.1007/BF00226622
更新日期:1977-09-26 00:00:00
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journal_title:Cell and tissue research
pub_type: 杂志文章
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