Abstract:
:CXCL2 (macrophage inflammatory protein-2 (MIP-2)), a critical chemokine for neutrophils, has been shown to be produced in the rat intestine in response to platelet-activating factor (PAF) and to mediate intestinal inflammation and injury. The intestinal epithelium, constantly exposed to bacterial products, is the first line of defence against micro-organisms. It has been reported that enterocytes produce proinflammatory mediators, including tumour necrosis factor (TNF) and PAF, and we showed that lipopolysaccharide (LPS) and TNF activate nuclear factor (NF)-kappaB in enterocytes. However, it remains elusive whether enterocytes release CXCL2 in response to LPS and TNF via a NF-kappaB-dependent pathway and whether this involves the endogenous production of TNF and PAF. In this study, we found that TNF and LPS markedly induced CXCL2 gene expression in IEC-6 cells, TNF within 30 min, peaking at 45 min, while LPS more slowly, peaking after 2 hr. TNF- and LPS- induced CXCL2 gene expression and protein release were completely blocked by pyrrolidine dithiocarbamate (PDTC) and helenalin, two potent NF-kappaB inhibitors. NEMO-binding domain peptide, a specific inhibitor of inhibitor protein kappaB kinase (IKK) activation, a major upstream kinase mediating NF-kappaB activation, significantly blocked CXCL2 gene expression and protein release induced by LPS. WEB2170 (PAF antagonist) and anti-TNF antibodies had no effect on LPS-induced CXCL2 expression. In conclusion, CXCL2 gene is expressed in enterocytes in response to both TNF and LPS. LPS-induced CXCL2 expression is dependent on NF-kappaB activation via the IKK pathway. The effect of LPS is independent of endogenous TNF and PAF.
journal_name
Immunologyjournal_title
Immunologyauthors
De Plaen IG,Han XB,Liu X,Hsueh W,Ghosh S,May MJdoi
10.1111/j.1365-2567.2006.02344.xsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
153-63issue
2eissn
0019-2805issn
1365-2567pii
IMM2344journal_volume
118pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Activation of human eosinophils by platelet-activating factor (PAF) involves multiple signal transduction pathways. Among these, protein kinase C has been demonstrated both to mediate respiratory burst and to suppress an alternative pathway of activation of respiratory burst and arachidonic acid metabolism in eosinoph...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00037.x
更新日期:2000-06-01 00:00:00
abstract::Ribonucleic acid (immune RNA, iRNA), extracted from the spleens of mice immunized with sheep red blood cells (SRBC), induced specific immunological memory. The humoral immune responses of iRNA-treated animals were compared with in vivo and in vitro primary and secondary responses, using the haemolytic plaque-forming c...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Over the past 10 years we have made great strides in our understanding of T helper cell differentiation, expansion and effector functions. Within the context of T helper type 2 (Th2) cell development, novel innate-like cells with the capacity to secrete large amounts of interleukin-5 (IL-5), IL-13 and IL-9 as well as ...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2011.03497.x
更新日期:2011-12-01 00:00:00
abstract::B-lymphocyte maturation is considered to be independent of the thymus. However, there is circumstantial evidence suggesting that it may be impaired in nude animals that lack the thymus. Our study shows that the proportion of immature B-lymphocyte subsets (CD90(high) IgM(high) and CD90(high) IgM(low)) was significantly...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02226.x
更新日期:2005-11-01 00:00:00
abstract::In an attempt to produce monoclonal antibodies against human large granular lymphocytes (LGL), the effector cells of natural killer (NK) and killer (K) activity, a monoclonal IgM antibody (VEP10) has been obtained. This antibody is reactive by indirect membrane immunofluorescence (IMF) with 14.7 +/- 8.5% peripheral bl...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::Treatment of patients with septic shock using monoclonal antibodies (mAbs) to endotoxin is still controversial. Clinical trials of E5, one of the mAbs directed against the lipid A moiety of lipopolysaccharide (LPS), are currently in progress. The mechanisms of action of this, and other antibodies under clinical evalua...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Recently it has been shown that selective subconjunctival macrophage depletion reduced the incidence and severity of stromal herpes simplex virus (HSV) keratitis in mice. In this study, we examined the effect of conjunctival macrophage depletion on the corneal and systemic T-cell-mediated immune response. BALB/c mice ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2002.01477.x
更新日期:2002-09-01 00:00:00
abstract::Cytotoxic T-lymphocytes (CTL) play an important role in the control of human immunodeficiency virus (HIV) and of human cytomegalovirus (HCMV) infection. Following highly active antiretroviral therapy (HAART), most studies have demonstrated a decline in the frequency of HIV-specific CTL. We analysed the effect of HAART...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02334.x
更新日期:2006-05-01 00:00:00
abstract::Both lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA) impeded monocyte to macrophage differentiation with respect to typical phenotypic modulation and certain phagocyte-related processes. The down-regulation of the porcine monocyte marker SWC1, and up-regulation of the SWC9 macrophage marker were ret...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01266.x
更新日期:2001-08-01 00:00:00
abstract::The haemolymph of the colonial ascidian Botrylloides leachii contains two haemagglutinins which have been termed HA-1 and HA-2 respectively. The HA-1 agglutinin is specific for guinea-pig erythrocytes, requires Ca2+ ions for its activity and has an apparent mol. wt. of about 200,000. It can be adsorbed by guinea-pig b...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
abstract::Corneal allografts contain few or no Ia+ Langerhans' cells and consistently fail to elicit allospecific delayed-type hypersensitivity (DTH). The present study examined the hypothesis that the inability of corneal allografts to induce allospecific DTH was the result of active suppression. However, the results indicated...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::C57BL/6 mice were injected with different doses of human T-cell receptor (TCR) V beta 8.1 CDR1 peptide at different times after murine retrovirus (LP-BM5) infection. Injection with TCR V beta 8.1 CDR1 peptide largely prevented the retrovirus-induced reduction in B- and T-cell proliferation, and T-helper 1 (Th1) cytoki...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.449551.x
更新日期:1996-02-01 00:00:00
abstract::N-Methyl-N-nitrosourea (MNU) but not methyl methanesulphonate (MMS) induced both autoimmune haemolytic anaemia and thymic lymphoma in susceptible strains of mice, particularly the C57BL/6. These effects could be positively correlated with the formation of O6-methylguanine in target DNA. All murine lymphoid cells showe...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::In order to study the precise localization pattern of anti-TNP antibody-forming cells (AFCs) during the early primary immune response against TNP conjugated TD (thymus-dependent) and TI-2 (thymus-independent type-2) antigens, rats received an intravenous injection with either TNP-keyhole limpet haemocyanin (KLH) or wi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Bovine Concanavalin A-induced suppressor cells were generated from lymphocytes which were non-adherent to anti-immunoglobulin coated dishes and cells possessing receptors for peanut agglutinin. Bovine lymphocytes, preincubated with 25 microgram/ml of Con A for 40-45 hr, could suppress the responses of autologous cells...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::The effect of invasive bacteria on the release of proinflammatory mediators (oxygen radicals, leukotriene release) from human polymorphonuclear neutrophils was studied. Bacterial stimuli were used including genetically cloned invasive Yersinia enterocolitica strains 108-P (bearing the phagocytosis-resistance plasmid) ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::It is increasingly becoming clear that various immune cells are infected by the very pathogens that they are supposed to attack. Although many mechanisms for microbial entry exist, it appears that a common route of entry shared by certain bacteria, viruses and parasites involves cellular lipid-rich microdomains someti...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2567.2001.01173.x
更新日期:2001-01-01 00:00:00
abstract::Functionally defined mouse Lyt-1 and Lyt-2 cell sets were selected from peripheral lymph nodes by cytotoxic elimination with anti-Lyt monoclonal antibodies. The selected populations were labelled with 51Cr or [3H]-adenosine and traced in syngeneic recipients at 1, 24, 45 and 65 hr after intravenous injection. Recovere...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::We studied proliferative responses of bovine T lymphocytes to foot-and-mouth disease virus (FMDV) serotypes A, O and C as well as to three peptides including the two major B-cell epitopes of FMDV (VP1[141-156] and VP1[200-213]). Peripheral blood mononuclear cells (PBMC) from cattle previously vaccinated with monovalen...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::Natural cell-mediated cytotoxicity is mediated by a family of effector cells that express cytolytic activities distinct from those generally attributed to B cells, T cells and macrophages; it includes both natural killer (NK) and natural cytotoxic (NC) activities. There is now convincing evidence to show that NC activ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-08-01 00:00:00
abstract::The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large g...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::Lymphocyte activation requires signal transduction mediated by reversible phosphorylation. Changing profiles of phosphorylated intermediates relate to the progressive series of transduction pathways in cells moving from G0 to G1, and thereafter through the cell cycle. We have previously shown that transient inhibition...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::Eosinophil function is regulated by several cytokines, including interleukin-3 (IL-3), IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF). Culture of human eosinophils with IL-3 produced a marked, dose-dependent up-regulation of CR3 expression. This was maximal after 1 day in culture and dependent on p...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
abstract::Using cloned human helper T lymphocytes reactive with a 24 amino acid peptide (p20) of the carboxyl terminal of the HA-1 molecule of influenza haemagglutinin we have investigated the influence of antigen structure on the activation and in the induction of antigen specific unresponsiveness of T cells. For this analysis...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Male and female spleen cells were compared before and after culture for their cytotoxicity to autologous-embryo fibroblasts. Cultures of male cells developed significantly greater reactivity than cultures of female cells. Moreover, while the cytotoxicity derived from cultures of male cells was totally abolished by tre...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::Free malarial merozoites isolated from in vitro cultures of P. knowlesi and emulsified with Freund's complete (FCA) or incomplete (FIA) adjuvant were used to vaccinate twelve Rhesus monkeys against the uniformly lethal infection caused by P. knowlesi. Initial challenge of six monkeys with the same parasite variant as ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Studies with gene-deficient and gnotobiotic mice have identified many host and microbial factors that contribute to induced colitis, but information on whether specific factors determine susceptibility under more physiological conditions is lacking. Using wild-type strains that differ in their IgA response but harbor ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13091
更新日期:2019-09-01 00:00:00
abstract::The in vitro migration activity of monocytic cells and the effect of lymphocytes in the delayed hypersensitivity (DH) reaction induced by intrapleural injection of PPD into FCA-sensitized guinea-pigs was investigated. The in vitro migration of exudate mononuclear cells was lower than that of comparable blood cells, an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::Cells subjected to nucleoside incorporation studies using radiolabelled materials may suffer radiation damage that can alter the results. We did scintimetric and cytofluorographic analysis to confirm this and to determine the optimal experimental doses of, and exposure times to, [3H]-TdR, in order to prevent or minimi...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::The cellular basis of the mechanism of immunological tolerance to human gamma-globulin (H gamma G) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated H gamma G) into the mothers was investigated using a cell transfer system and assays ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00