Abstract：
:Group I and II introns are mobile elements that propagate by insertion into different genes. Some introns of both types self-splice in vitro by transesterification reactions catalysed by the intron RNA. These transesterifications are reversible, and it has been suggested that reverse splicing followed by reverse transcription and recombination with genomic DNA may be a mechanism for intron transposition. In vivo the splicing of many, if not all, group I and II introns requires protein factors, which may facilitate correct folding of the intron RNAs. Here we show that the Neurospora mitochondrial large rRNA intron, a group I intron that is not self-splicing in vitro, undergoes reverse splicing in a reaction promoted by the CYT-18 protein, the Neurospora mitochondrial tyrosyl-tRNA synthetase, which is required for splicing the intron in vivo. In contrast to known RNA-catalysed reverse splicing reactions, this protein-assisted reverse splicing is sufficiently rapid to compete with forward splicing at low RNA concentrations under physiologically relevant conditions, including high GTP and low Mg2+ concentrations. Our results indicate that proteins that promote splicing could contribute to intron mobility by promoting reverse splicing in vivo.
journal_name
Naturejournal_title
Natureauthors
Mohr G,Lambowitz AMdoi
10.1038/354164a0keywords:
subject
Has Abstractpub_date
1991-11-14 00:00:00pages
164-7issue
6349eissn
0028-0836issn
1476-4687journal_volume
354pub_type
杂志文章相关文献
NATURE文献大全abstract：:oop RNA, which is initiated at the po promoter and is 81 nucleotides long, can function as a leader sequence for the lambda immunity establishment transcription, previously believed to originate at a special promoter pre located in the y region. Thus, oop RNA seems to have a dual role, either favouring the lytic cycle...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/262112a0
更新日期：1976-07-08 00:00:00
abstract：:Complex-oxide materials exhibit a vast range of functional properties desirable for next-generation electronic, spintronic, magnetoelectric, neuromorphic, and energy conversion storage devices1-4. Their physical functionalities can be coupled by stacking layers of such materials to create heterostructures and can be f...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-1939-z
更新日期：2020-02-01 00:00:00
abstract：:Long-term reconstitution of the lymphohaematopoietic cells of a mouse after lethal irradiation requires the transplantation of at least (5-10) x 10(3) bone marrow cells. Several cell-separation techniques based on cell-surface characteristics have been used in attempts to identify the pluripotent haematopoietic stem c...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347188a0
更新日期：1990-09-13 00:00:00
abstract：:Receptors for dopamine have been classified into two functional types, D1 and D2. They belong to the family of receptors acting through G (or guanine nucleotide-binding) proteins. D2 receptors inhibit adenylyl cyclase, but D1 receptors stimulate adenylyl cyclase and activate cyclic AMP-dependent protein kinases. Dopam...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347080a0
更新日期：1990-09-06 00:00:00
abstract：:The proto-oncogene c-jun is the cellular homologue of v-jun, the transforming oncogene of the avian sarcoma virus 17 (ref. 1). c-jun encodes one major component of the AP-1 transcription factor complex and is expressed in many organs during mouse development and in the adult. Because of its rapid induction in cells fo...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/365179a0
更新日期：1993-09-09 00:00:00
abstract：:The human T cell erythrocyte receptor (CD2 antigen) allows thymocytes and mature T cells to adhere to thymic epithelium and target cells through a cell surface protein, LFA-3 (refs 1-6). Monoclonal antibodies recognizing CD2 can either block adhesion or, in certain combinations, induce an antigen-independent T cell ac...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/329842a0
更新日期：1987-10-29 00:00:00
abstract：:During platelet activation there is a major reorganization in the platelet cytoskeleton that accompanies a rapid change in platelet shape. Many of the events associated with activation are attributed to a rise in calcium concentration within the platelet cytoplasm. One direct consequence of the elevated calcium is the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/317449a0
更新日期：1985-10-03 00:00:00
abstract：:Mammalian cells proteolytically release (shed) the extracellular domains of many cell-surface proteins. Modification of the cell surface in this way can alter the cell's responsiveness to its environment and release potent soluble regulatory factors. The release of soluble tumour-necrosis factor-alpha (TNF-alpha) from...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/385729a0
更新日期：1997-02-20 00:00:00
abstract：:Two approaches to the antibody-directed targeting of toxic or cytolytic activity and augmentation of cellular immune responses have been explored for tumour immunotherapy, but so far success has been limited. Obstacles facing immunotherapy are the limited accessibility of antibodies or antibody conjugates to solid tum...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/385078a0
更新日期：1997-01-02 00:00:00
abstract：:'Pioneer' transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming1,2. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-re...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2195-y
更新日期：2020-04-01 00:00:00
abstract：:Wrists, ankles and digits distinguish tetrapod limbs from fins, but direct evidence on the origin of these features has been unavailable. Here we describe the pectoral appendage of a member of the sister group of tetrapods, Tiktaalik roseae, which is morphologically and functionally transitional between a fin and a li...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/nature04637
更新日期：2006-04-06 00:00:00
abstract：:The T-cell specific, rearranging gamma-chain genes bear striking resemblance to T-cell receptor and immunoglobulin genes, but the role of gamma remains unknown. A central problem is to understand the conditions under which gamma RNA is expressed in cells. The transcription of gamma is abundant in T cells of fetal thym...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/323635a0
更新日期：1986-10-16 00:00:00
abstract：:Lyman-alpha emitters are thought to be young, low-mass galaxies with ages of approximately 10(8) yr (refs 1, 2). An overdensity of them in one region of the sky (the SSA 22 field) traces out a filamentary structure in the early Universe at a redshift of z approximately 3.1 (equivalent to 15 per cent of the age of the ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature07947
更新日期：2009-05-07 00:00:00
abstract：:Somatic cell nuclear transfer (SCNT) into enucleated oocytes has emerged as a technique that can be used to derive mouse embryonic stem cell lines with defined genotypes. In this issue Byrne et al. report the derivation of two SCNT Rhesus macaca male stem cell lines designated CRES-1 and CRES-2. Molecular studies deta...
journal_title：Nature
pub_type： 评论,杂志文章
doi：10.1038/nature06456
更新日期：2007-11-22 00:00:00
abstract：:How black holes accrete surrounding matter is a fundamental yet unsolved question in astrophysics. It is generally believed that matter is absorbed into black holes via accretion disks, the state of which depends primarily on the mass-accretion rate. When this rate approaches the critical rate (the Eddington limit), t...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature16452
更新日期：2016-01-07 00:00:00
abstract：:The effect of anthropogenic aerosols on cloud droplet concentrations and radiative properties is the source of one of the largest uncertainties in the radiative forcing of climate over the industrial period. This uncertainty affects our ability to estimate how sensitive the climate is to greenhouse gas emissions. Here...
journal_title：Nature
pub_type： 历史文章,杂志文章
doi：10.1038/nature12674
更新日期：2013-11-07 00:00:00
abstract：:The ability to process microbial antigens and present them at the surface of cells is an important aspect of our innate ability to clear infections. It is generally accepted that antigens in the cytoplasm are loaded in the endoplasmic reticulum and presented at the cell surface on major histocompatibility complex (MHC...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01912
更新日期：2003-09-25 00:00:00
abstract：:The yeast nuclear protein Yra1p is an essential export factor for mRNA. Yra1p interacts directly with the mRNA transport factor Mex67p/Mtr2p, which is associated with the nuclear pore. Here, we report a genetic interaction between YRA1 and SUB2, the gene for a DEAD box helicase involved in splicing. Mutation of SUB2 a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35098113
更新日期：2001-10-11 00:00:00
abstract：:The most recent Ebola virus outbreak in West Africa, which was unprecedented in the number of cases and fatalities, geographic distribution, and number of nations affected, highlights the need for safe, effective, and readily available antiviral agents for treatment and prevention of acute Ebola virus (EBOV) disease (...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature17180
更新日期：2016-03-17 00:00:00
abstract：:Epstein-Barr virus (EBV), a member of the herpes group of viruses and the aetiological agent of infectious mononucleosis, is usually thought of as a lymphotrophic virus with the ability to transform B lymphocytes. So the association of EBV with nasopharyngeal carcinoma is puzzling, especially given the lack of success...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/306480a0
更新日期：1983-12-01 00:00:00
abstract：:Large, actively swimming suspension feeders evolved several times in Earth's history, arising independently from groups as diverse as sharks, rays and stem teleost fishes, and in mysticete whales. However, animals occupying this niche have not been identified from the early Palaeozoic era. Anomalocarids, a group of st...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13010
更新日期：2014-03-27 00:00:00
abstract：:The crystal structure of a solid controls the interactions between the electronically active units and thus its electronic properties. In the high-temperature superconducting copper oxides, only one spatial arrangement of the electronically active Cu(2+) units-a two-dimensional square lattice-is available to study the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09120
更新日期：2010-07-08 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 新闻
doi：10.1038/d41586-018-07480-0
更新日期：2018-11-01 00:00:00
abstract：:Sleep is one of the few major whole-organ phenomena for which no function and no underlying mechanism have been conclusively demonstrated. Sleep could result from global changes in the brain during wakefulness or it could be regulated by specific loci that recruit the rest of the brain into the electrical and metaboli...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature04811
更新日期：2006-06-08 00:00:00
abstract：:Ten eleven translocation (TET) enzymes, including TET1, TET2 and TET3, convert 5-methylcytosine to 5-hydroxymethylcytosine and regulate gene transcription. However, the molecular mechanism by which TET family enzymes regulate gene transcription remains elusive. Using protein affinity purification, here we search for f...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11742
更新日期：2013-01-24 00:00:00
abstract：:For unknown reasons, autoimmune diseases such as type 1 diabetes develop after prolonged periods of inflammation of mononuclear cells in target tissues. Here we show that progression of pancreatic islet inflammation to overt diabetes in nonobese diabetic (NOD) mice is driven by the 'avidity maturation' of a prevailing...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35021081
更新日期：2000-08-17 00:00:00
abstract：:The protein HLA-E is a non-classical major histocompatibility complex (MHC) molecule of limited sequence variability. Its expression on the cell surface is regulated by the binding of peptides derived from the signal sequence of some other MHC class I molecules. Here we report the identification of ligands for HLA-E. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35869
更新日期：1998-02-19 00:00:00
abstract：:Many homeobox genes are clustered in a linear array along a chromosome, reflecting their ordered expression along the anterior-posterior axis of the embryo. Expression patterns as well as grafting, ectopic expression and loss-of-function experiments suggest that the Hox genes encode a combinatorial system of positiona...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/368747a0
更新日期：1994-04-21 00:00:00
abstract：:The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage. Previously, it has been assumed that RecA binds to the DNA template strand being copied. Here we show, however, that pol-V-catalysed translesion syn...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05042
更新日期：2006-08-24 00:00:00
abstract：:The snowball Earth hypothesis postulates that the planet was entirely covered by ice for millions of years in the Neoproterozoic era, in a self-enhanced glaciation caused by the high albedo of the ice-covered planet. In a hard-snowball picture, the subsequent rapid unfreezing resulted from an ultra-greenhouse event at...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10499
更新日期：2011-10-05 00:00:00
