Abstract：
:The native retinal pigment epithelium (RPE) exists as a monolayer structure and is critically involved in the maintenance of photoreceptors. Damage or destruction of the RPE due to a variety of diseases therefore often results in loss of vision. With regenerative purposes in mind, we have examined various culture conditions such as the initial cell density and the addition of various supplements in an effort to produce transplantable RPE cell sheets that can be harvested without defects. We demonstrate that the cell density in cultured RPE sheets increased linearly with the number of seeded cells and that RPE sheets were harvested without defects and limited contraction due to cytoskeletal reorganization, when TGF-beta2 was added to the growth medium. Results from histological analysis and the measurement of trans-epithelial resistance also demonstrates that these RPE cell sheets exist as monolayer structure, similar to the native RPE, with intact cell-to-cell junctions. Therefore, these methods provide significant insight into the fabrication of transplantable RPE cell sheets that can be applied to RPE regenerative therapies to restore lost vision.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Kubota A,Nishida K,Yamato M,Yang J,Kikuchi A,Okano T,Tano Ydoi
10.1016/j.biomaterials.2006.02.018keywords:
subject
Has Abstractpub_date
2006-07-01 00:00:00pages
3639-44issue
19eissn
0142-9612issn
1878-5905pii
S0142-9612(06)00171-2journal_volume
27pub_type
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