Abstract:
:Glucose oxidase was immobilized into photographic gelatin hardened by chromium(III) sulphate. The enzyme-gelatin mixture was coated on polyester film strips which allowed easy and simple handling during assays. The effect of gelatin and cross-linker concentrations on water content and enzymatic activity was studied. The effect of pH during immobilization and that of incubation temperature on maximum activity were examined. Enzyme leakage tests were carried out during reuse number studies. Consecutive use of strips followed by washing and resting between uses were found to affect the reuse number. A maximum immobilization of 68% was reached under optimal conditions. Mechanical stability and leakage were found to be functions of gelatin and cross-linker concentrations. Photographic gelatin was found to have many capabilities with extraordinary characteristics as a carrier on immobilization.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Elçin YM,Akbulut Udoi
10.1016/0142-9612(92)90064-ukeywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
156-61issue
3eissn
0142-9612issn
1878-5905pii
0142-9612(92)90064-Ujournal_volume
13pub_type
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