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Cryopreservation and CO2-independent culture of 3D cardiac progenitors for spaceflight experiments.

Abstract：

:Space experimentation of cardiomyocyte differentiation from human induced pluripotent stem cells offers an exciting opportunity to explore the potential of these cells for disease modeling, drug discovery and regenerative medicine. Previous studies on the International Space Station were done with 2D non-cryopreserved cultures of cardiomyocytes being loaded and cultivated in spaceflight culture modules with CO2. Here we report the development of methods of cryopreservation and CO2-independent culture of 3D cardiac progenitors. The cryopreservation allows preparation and pretesting of the cells before spaceflight, makes it easier to transport the cell culture, reduces the impact of strong gravitational force exerted on the cells during the launch of spaceflight, and accommodates a more flexible working schedule for the astronauts. The use of CO2-independent medium with supplements supports cell growth and differentiation without a CO2 incubator. With these methods, we conducted a spaceflight experiment through the SpaceX-20 mission to evaluate the effect of microgravity on the survival and differentiation of 3D cardiac progenitors. Our cryopreserved cardiac progenitor spheres were successfully cultivated in a spaceflight culture module without CO2 for 3 weeks aboard the International Space Station. Beating cardiomyocytes were generated and returned to the earth for further study.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Rampoldi A,Jha R,Fite J,Boland G,Xu C

doi

10.1016/j.biomaterials.2021.120673

subject

Has Abstract

pub_date

2021-01-11 00:00:00

pages

120673

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(21)00024-7

journal_volume

269

pub_type

杂志文章

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