Abstract:
:The objective of this study was to evaluate abomasal cytokine responses in helminth-naive calves and calves vaccinated with protective antigen fractions from Ostertagia ostertagi after an experimental challenge infection with infective third stage (L3) larvae. Abomasal lymph nodes and/or abomasal mucosa were collected and messenger RNA for the Th1 cytokines (IFN-gamma, IL-2, IL-12 p40 subunit), the Th2 cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IL-15) and the Th3/Tr cytokine TGF-beta was quantified by real-time RT-PCR. Vaccination had no effect on cytokine profiles in either the abomasal lymph nodes or the abomasal mucosa. However, following infection all calves showed a significant decrease in the Th1 cytokines, IFN-gamma and IL-12 p40, and a significant increase in the Th2 cytokines, IL-4, IL-5, IL-10 and IL-13 in the lymph nodes, compared to non-infected calves. No correlation between the Th2 response and protection induced by vaccination could be demonstrated. In contrast, a Th2 pattern was not observed in the mucosa of the infected calves, which exhibited an increase in IFN-gamma as well as in the Th2 cytokines IL-4, IL-5 and IL-10 mRNA. No significant association was observed in the abomasal mucosa between any examined cytokine mRNA level and immune effector responses such as parasite-specific antibodies or the number of mucosal mast cells or eosinophils.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Claerebout E,Vercauteren I,Geldhof P,Olbrechts A,Zarlenga DS,Goddeeris BM,Vercruysse Jdoi
10.1111/j.1365-3024.2005.00780.xkeywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
325-31issue
9eissn
0141-9838issn
1365-3024pii
PIM780journal_volume
27pub_type
杂志文章abstract:AIMS:Schistosomiasis and malaria are endemic in sub-Saharan Africa where Schistosoma haematobium (Sh) and Plasmodium falciparum (Pf) coinfections are thus frequent. We explored the effect of Sh infection on antibody responses directed to Pf merozoite antigens and on malaria susceptibility in Beninese children. METHODS...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12702
更新日期:2020-04-01 00:00:00
abstract::A surface enriched fraction was prepared from adults of Onchocerca volvulus by brief extraction of entire worms with detergent. This was then gel filtered to yield a low molecular weight fraction which functioned specifically in ELISA analysis. An identical result was also obtained when the related cattle parasite, O....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1987.tb00487.x
更新日期:1987-01-01 00:00:00
abstract::ES-62 is a glycoprotein secreted by the filarial nematode Acanthocheilonema viteae that protects against ovalbumin (OVA)-induced airway hyper-responsiveness in mice by virtue of covalently attached anti-inflammatory phosphorylcholine (PC) residues. We have recently generated a library of small molecule analogues (SMAs...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12322
更新日期:2016-06-01 00:00:00
abstract::On a B10 (Lshs) genetic background, the development of acquired T cell mediated immunity to Leishmania donovani infection in mice is under H-2 linked genetic control. Following intravenous inoculation of 10(7) amastigotes three phenotypic patterns of recovery have been described: 'early cure' (H-2r,s), 'cure' (H-2b) a...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00760.x
更新日期:1983-09-01 00:00:00
abstract::The prostaglandins (PG) are known to regulate immune cell function(s) and participate in the progression of both acute and chronic inflammatory reactions. Using an in vitro model of Schistosoma mansoni egg-induced hypersensitivity granulomas, we have delineated the role of immune complexes (IC) in the induction and re...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00299.x
更新日期:1994-01-01 00:00:00
abstract::The in vitro binding serum complement (C) components to Schistosoma mansoni worms was studied. By exposing frozen sections of adult male and female parasites to normal human serum, binding of both classical and alternative pathway C components was observed. By immunofluorescence (IFL) microscopy selective binding of C...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00736.x
更新日期:1983-03-01 00:00:00
abstract::In the present study we have investigated and compared in vitro the specific complement (C) activating activity of three metacestode preparations of Echinococcus granulosus. Extracts from hydatid cyst fluid (HCF-ext), protoscoleces (PSC-ext) and hydatid cyst membrane (HCM-ext) activated human C producing C3 conversion...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-112.x
更新日期:1996-07-01 00:00:00
abstract::African trypanosomes are well known for their ability to avoid immune elimination by switching the immunodominant variant surface glycoprotein (VSG) coat during infection. However, antigenic variation is only one of several means by which trypanosomes manipulate the immune system of their hosts. In this article, the r...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2005.00791.x
更新日期:2005-10-01 00:00:00
abstract::African trypanosomes cause human and animal African trypanosomiases, which are chronic, debilitating and often fatal diseases of people and livestock in sub-Saharan Africa. The extracellular protozoan parasites are exemplars of antigenic variation. They direct host-protective B-cell and T-cell immune responses towards...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12387
更新日期:2016-12-01 00:00:00
abstract::UV-irradiated and gamma-irradiated schistosomula of Schistosoma mansoni induce high levels of resistance to challenge infection in experimental hosts. It was observed that both types of irradiation severely inhibited protein synthesis by the parasite larvae. Schistosomula were treated with the metabolic inhibitor acti...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1992.tb00025.x
更新日期:1992-09-01 00:00:00
abstract::Humoral immunity develops in the spleen during blood-stage Plasmodium infection. This elicits parasite-specific IgM and IgG, which control parasites and protect against malaria. Studies in mice have elucidated cells and molecules driving humoral immunity to Plasmodium, including CD4+ T cells, B cells, interleukin (IL)...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12455
更新日期:2017-10-01 00:00:00
abstract::The life cycle of Taenia solium, the pork tapeworm, is continuously closed in many rural settings in developing countries when free roaming pigs ingest human stools containing T. solium eggs and develop cysticercosis, and humans ingest pork infected with cystic larvae and develop intestinal taeniasis, or may also acci...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12126
更新日期:2014-08-01 00:00:00
abstract::The kinetics of macrophage activation were compared among inbred strains of mice (C3H, BALB, B6 and B10.A) that are known to differ in their relative resistance to infections with the myotropic strains (Colombian and CL) of Trypanosoma cruzi. The parameters utilized to measure macrophage activation were rapid spreadin...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00675.x
更新日期:1989-07-01 00:00:00
abstract::Carboxy-terminus of merozoite surface protein-1 (MSP-1(19) ) is the major protein on the surface of the plasmodial merozoite that acts as one of the most important blood-stage vaccine candidates. The present investigation was designed to evaluate the immune responses when either two recombinant antigens (rPvMSP-1(19) ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01331.x
更新日期:2011-11-01 00:00:00
abstract::Entamoeba histolytica is the aetiological agent of invasive amoebiasis, the third leading parasitic cause of mortality in the world. The disease can be easily cured by chemotherapy; however, prevention, mainly in the form of vaccination, could greatly decrease the incidence of the disease, and possibly help in its era...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-3024.2003.00607.x
更新日期:2003-02-01 00:00:00
abstract::Activation of macrophages is a key step in the initiation of immune responses, but the transcriptional mechanisms governing macrophage activation during infection are not fully understood. It was recently shown that the AP-1 family transcription factor JUNB positively regulates macrophage activation in response to Tol...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12215
更新日期:2015-09-01 00:00:00
abstract::The humoral responses in mice following vaccination with DNA constructs encoding Fasciola hepatica glutathione S-transferase (GST) have been evaluated. GST47 cDNA was subcloned into two DNA vaccine vectors, VR1012 and VR1020, which direct expression to the cytoplasmic and extracellular compartments, respectively. Expr...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00235.x
更新日期:1999-07-01 00:00:00
abstract::Seven mixed-breed dogs were challenged with either promastigotes or amastigotes of Leishmania donovani infantum strains recently isolated from naturally infected dogs. Different routes and numbers of parasites were utilized and each dog was monitored for at least 1 year post-infection. Anti-parasite specific antibody ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1991.tb00550.x
更新日期:1991-09-01 00:00:00
abstract::A comparison of Plasmodium chabaudi AS infection in BALB/c and BALB/c IgM-deficient mice demonstrated a protective role for IgM during infection. IgM-/- mice, unlike microMT mice, display competent B cell humoral immune responses. Increased susceptibility of IgM-/- mice was demonstrated by increased mortality, an adva...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2005.00760.x
更新日期:2005-05-01 00:00:00
abstract::Immunoprecipitations of in vitro translation products of mRNA from adult Schistosoma mansoni worms with sera from chronically infected mice of 12 inbred strains reveal a strain-dependent heterogeneity in the antibody responses to schistosome antigens. Of the strains tested the response to the 86K antigen was restricte...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00870.x
更新日期:1986-11-01 00:00:00
abstract::The role of MHC class II in the presentation of Heligmosomoides polygyrus antigens has been investigated, using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen presenting cells (APC), further evidence has emerged, to support earlier data, that there c...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1996.tb01028.x
更新日期:1996-09-01 00:00:00
abstract::Use of the pig as an animal model in schistosomiasis research is increasing, but knowledge of the porcine immune response to schistosome infection is still very limited. We investigated the immunohistology of different maturation stages of the Schistosoma japonicum egg granuloma in pigs. Liver sections from pigs exper...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00448.x
更新日期:2002-03-01 00:00:00
abstract::The carrier effect, using TNP-labelled schistosomula was used to measure the helper T-cell activity against the schistosomula surface in CBA mice exposed to 30 cercariae of Schistosoma mansoni. After infection the helper T-cell activity reached a peak in 8--10 days, but by 6 weeks it had declined to background levels....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1981.tb00401.x
更新日期:1981-10-01 00:00:00
abstract::A 17 kD protein of Necator americanus was isolated by SDS-PAGE and used to raise monospecific antisera in rabbits. ELISA and Western blotting against a range of parasite extracts demonstrated the species specificity of this protein. It is expressed at all stages of the life-cycle, appears to be accumulated through the...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00953.x
更新日期:1990-05-01 00:00:00
abstract::Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb-S). Of several adjuvants tested, including Freund's, BCG and alum, 50 micrograms of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approac...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00669.x
更新日期:1989-07-01 00:00:00
abstract::Cryptosporidiosis is a zoonotic protozoan disease that affects the gastrointestinal tract of animals and humans. Diarrhoea as the most important indication of the infection leads to high economic losses in livestock industries and is a life threatening infection in immunocompromised individuals. In the absence of the ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12317
更新日期:2016-05-01 00:00:00
abstract::Nitric oxide (NO) plays diverse roles in a variety of pathological processes. We investigated the role of NO in Schistosoma japonicum egg-induced granuloma formation in a mouse hepatic model. Immunohistological analysis revealed that there is the most intense and extensive inducible nitric oxide (iNOS) expression 2 we...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2001.00384.x
更新日期:2001-06-01 00:00:00
abstract::Haemonchus contortus is an economically important parasite that survives the host defense system by modulating the immune response. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is secreted by the parasite and the host responds by producing anti-enzyme antibodies. The enzyme inhibits complement cascade, an arm of t...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12611
更新日期:2019-02-01 00:00:00
abstract::Leishmania mexicana is the causal agent of cutaneous leishmaniasis in Mexico. Dendritic cells (DC) are one of the host cells of Leishmania parasites. Intracellular microorganisms inhibit host cell apoptosis as a strategy to ensure their survival in infected cells. We have previously shown that Leishmania mexicana prom...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12275
更新日期:2015-11-01 00:00:00
abstract::The effect of infection and subsequent challenge with Eimeria maxima on the populations of lymphocytes in the small intestine of Light Sussex chickens was assessed by immunohistochemistry. T cells were characterized for CD3, CD4, CD8, TCR1 (gamma delta heterodimer) or TCR2 (alpha beta 1 heterodimer) markers, and B cel...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00883.x
更新日期:1995-10-01 00:00:00