Abstract:
:A surface enriched fraction was prepared from adults of Onchocerca volvulus by brief extraction of entire worms with detergent. This was then gel filtered to yield a low molecular weight fraction which functioned specifically in ELISA analysis. An identical result was also obtained when the related cattle parasite, O. gibsoni, was similarly fractionated and tested. The low molecular weight fraction contained at least four antigenic components when examined by coprecipitation and immunoblotting studies. One ml of packed worms yielded sufficient low molecular weight antigen to examine about 2,000 human sera by the ELISA procedure, and the test was sensitive at human serum dilutions down to 1/400. A preliminary study with individual sera from Onchocerciasis endemic and non-endemic areas of Southern Mexico yielded 0/24 false positives, 3/24 false negatives and a significant ELISA value in 21/24 sera from proven cases of Onchocerciasis.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Cabrera Z,Parkhouse RMdoi
10.1111/j.1365-3024.1987.tb00487.xsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
39-48issue
1eissn
0141-9838issn
1365-3024journal_volume
9pub_type
杂志文章abstract::Infection with Trypanosoma cruzi was established in the reduviid vector, Dipetalogaster maximus, by repeated feedings on mice with high parasitaemias, and metacyclic trypomastigotes (IMT) were collected in insect urine after blood meals. The infectivity of IMT in mice was assessed by placing varying numbers of organis...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00936.x
更新日期:1990-01-01 00:00:00
abstract::T-cell-deprived CBA mice were given thymus graft implants during the course of an infection with Trypanosoma musculi and at a time when the parasitaemia was high. As a consequence of the grafts, most of the mice survived the infection whereas control ungrafted mice died. In grafted animals there was generally a period...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1981.tb00413.x
更新日期:1981-01-01 00:00:00
abstract::The chromadorea abundant larval transcript (ALT) family of proteins contains ALT one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of Wuchereria bancrofti 20/22 (Wb20/22) as a member of chromadorea, the ALT family of proteins from the L3 stage of W. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12120
更新日期:2014-10-01 00:00:00
abstract::The zymosan-triggered chemiluminescence response of peritoneal exudate cells from mice infected with relatively virulent or avirulent Toxoplasma gondii strains was examined at various intervals after infection. Toxoplasma infections increased the background chemiluminescence compared with controls, but higher values w...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1984.tb00796.x
更新日期:1984-05-01 00:00:00
abstract::Attempts to produce monoclonal antibodies specific for a parasite-encoded or induced antigen on the surface of Theileria parva lawrencei-transformed bovine lymphocytes resulted in the identification of the monoclonal antibody J7. This antibody recognizes a membrane glycoprotein on some T.p. lawrencei-infected bovine l...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00841.x
更新日期:1986-03-01 00:00:00
abstract::Fasciola hepatica infection has been shown to suppress sheep lymphocyte proliferation in vitro and this is at least partially attributable to excretory/secretory products (ES) released by F. hepatica parasites. We identified a suppressive component in ES by analysing the effect of ES fractions, separated by gel filtra...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.0141-9838.2001.00438.x
更新日期:2002-02-01 00:00:00
abstract::The opportunistic protozoan parasite Toxoplasma gondii is well known as a strong inducer of cell-mediated immunity, largely as a result of proinflammatory cytokine induction during in vivo infection. Yet, during intracellular infection the parasite suppresses signal transduction pathways leading to these proinflammato...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01122.x
更新日期:2009-12-01 00:00:00
abstract::Fulani and Masaleit, sympatric tribes in eastern Sudan, are characterized by marked differences in susceptibility to Plasmodium falciparum malaria. To determine whether the two tribes differ in the frequency of immunoglobulin GM/KM allotypes, which are associated with immunity to several pathogens, serum samples from ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00938.x
更新日期:2007-05-01 00:00:00
abstract::Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb-S). Of several adjuvants tested, including Freund's, BCG and alum, 50 micrograms of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approac...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00669.x
更新日期:1989-07-01 00:00:00
abstract::Male B10 X 129 (10M) ScSn mice were relatively resistant to cutaneous leishmaniasis, while females frequently developed non-healing expanding ulcers, leading to loss of infected extremities, metastasis to distal skin sites, and in some animals, death. Anti-leishmanial antibody titers were higher, and delayed-type hype...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00831.x
更新日期:1986-01-01 00:00:00
abstract::In endemic areas, asymptomatic infection by the malaria parasite Plasmodium falciparum was found associated with elevated percentages of human host's mononuclear cell spontaneous in-vitro apoptosis. In Dielmo, a village where malaria is holoendemic, apoptosis was age-and parasite-dependent. In-vitro exposure of periph...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2000.00307.x
更新日期:2000-06-01 00:00:00
abstract::Immunoprecipitations of in vitro translation products of mRNA from adult Schistosoma mansoni worms with sera from chronically infected mice of 12 inbred strains reveal a strain-dependent heterogeneity in the antibody responses to schistosome antigens. Of the strains tested the response to the 86K antigen was restricte...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00870.x
更新日期:1986-11-01 00:00:00
abstract::Leishmaniasis has recently garnered attention as one of the diseases 'most neglected' by drug research and development, as the current therapeutic modalities available for the patients are ridden with unacceptable toxicity due to high dosage of the drug, prolonged treatment schedules, resistance and prohibitive costs....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12071
更新日期:2014-01-01 00:00:00
abstract::Cyclophosphamide (Cy) is known to produce a marked eosinophilia in mice after antigen challenge. In this study, we studied the effect of Cy on eosinophilia in helminthic infection. Peripheral blood eosinophils were counted in SJL mice following a large dose of Cy injection, infection with Toxocara canis, and both Cy i...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00074.x
更新日期:1985-05-01 00:00:00
abstract::Infections with the nematode Nematospiroides dubius fail to elicit mucosal mast cell (MMC) responses in the intestines of host mice, and suppress MMC responses generated by heterologous infection. Larval N. dubius have the capacity to prime for mastocytosis, and to elicit this response in primed mice during a challeng...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1988.tb00205.x
更新日期:1988-01-01 00:00:00
abstract::Inhibitory monoclonal antibody (MoAb) 8E7/55 recognizes a parasitophorous vacuole membrane (PVM) antigen in Plasmodium falciparum. Previous studies have identified the epitope, DNNLVSGP, recognized by the MoAb. A synthetic peptide containing this sequence was synthesized and coupled to diphtheria toxoid (DT) and was f...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00574.x
更新日期:1993-11-01 00:00:00
abstract::As an orally acquired pathogen, the immune response to Toxoplasma gondii unfolds in the small intestinal mucosa. There, an array of regulatory and effector immune cells are elicited to combat the parasite through secretion of inflammatory mediators, normally resulting in host protection and pathogen control. Recent st...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12164
更新日期:2015-03-01 00:00:00
abstract::Studies to clarify the mechanisms of apoptosis in host cells, A31 (BALB/3T3 clone A31 fibroblasts), caused by two intracellular protozoan parasites, Toxoplasma gondii and Neospora caninum, were carried out in an in vitro system. The viability of N. caninum-infected cells was significantly reduced following treatment w...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00476.x
更新日期:2002-07-01 00:00:00
abstract::CD4(+) T cells play a critical role in determining the disease outcome in murine cutaneous leishmaniasis, and selective usage of T-cell receptor (TCR) is implied in promoting Leishmania major infection. However, little information is available on TCR usage in Leishmania-specific, IFN-γ-producing CD4(+) T cells. In thi...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2010.01268.x
更新日期:2011-03-01 00:00:00
abstract::An essential element for continuing transmission of Plasmodium falciparum is the availability of mature gametocytes in human peripheral circulation for uptake by mosquitoes. Natural immune responses to circulating gametocytes may play a role in reducing transmission from humans to mosquitoes. Here, antibody recognitio...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12323
更新日期:2016-06-01 00:00:00
abstract::TSOL18 is a recombinant protein that has been shown in repeated experimental trials to be capable of protecting pigs against challenge infection with the cestode parasite Taenia solium. Antibodies raised by the vaccine are capable of killing the parasite in an in vitro culture and it is believed that antibody and comp...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01197.x
更新日期:2010-06-01 00:00:00
abstract::Changes in phagocytic and adherent cell numbers were compared during the course of infections of mice with Plasmodium yoelii (Py) and P. berghei (Pb) and in vaccinated mice challenged with homologous parasites. Nucleated cells in the spleen increased in number in Py-infected mice and were maximal at the time of recove...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00696.x
更新日期:1979-04-01 00:00:00
abstract::The K1 peptide is a CD8(+)T cell HLA-A*0201-restricted epitope derived from the Trypanosoma cruzi KMP-11 protein. We have previously shown that this peptide induces IFN-gamma secretion by CD8(+)T cells. The aim of this study was to characterize the frequency of K1-specific CD8(+)T cells in chagasic patients. Nineteen ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2010.01206.x
更新日期:2010-07-01 00:00:00
abstract::The merozoite surface protein 2 of P. falciparum is highly polymorphic in nature, but has regions of almost complete conservation at its N- and C-termini. We produced a chimeric recombinant protein comprising these regions only (hereafter termed NC). Mice immunized with the NC antigen produce antibodies at levels comp...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2000.00293.x
更新日期:2000-05-01 00:00:00
abstract::Dipetalonema viteae (Filarioidea) infections were established in inbred strains of mice by the s.c. implantation of adult female worms and the resulting microfilaraemia and adult worm survival monitored. BALB/c mice were the most susceptible strain examined, showing a high level microfilaraemia of approximately 6 mont...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00081.x
更新日期:1985-07-01 00:00:00
abstract::Infective larvae of Toxocara canis are well suited for studies of nematode antigen expression in vitro. Larvae were labelled with 3H-glucosamine, an approach permitting dual analysis of antigen quantity and composition. Their excretory/secretory (E/S) glycoproteins were efficiently labelled and antigen identity confir...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00946.x
更新日期:1990-03-01 00:00:00
abstract::Mice infected with lethal P. berghei or P. yoelii malaria develop anti-lymphocyte autoantibodies less than a week after infection. The autoantibodies are IgM, T-dependent and cytotoxic to a subpopulation of non-T lymphocytes at 37 degrees C in the presence of mouse or guinea-pig complement. Non-lethal P. yoelii and P....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1983.tb00742.x
更新日期:1983-05-01 00:00:00
abstract::UV-irradiated and gamma-irradiated schistosomula of Schistosoma mansoni induce high levels of resistance to challenge infection in experimental hosts. It was observed that both types of irradiation severely inhibited protein synthesis by the parasite larvae. Schistosomula were treated with the metabolic inhibitor acti...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1992.tb00025.x
更新日期:1992-09-01 00:00:00
abstract::Parasitic nematodes of the small-ruminant gastrointestinal tract pose a problem worldwide. The impact of these pathogens is worsened by the emergence of anthelmintic resistance to all three available classes of drugs. In addition to causing considerable economic loss, these parasites are detrimental to the health and ...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01110.x
更新日期:2009-07-01 00:00:00
abstract::The anti-leishmanial effect of the 'carbohydrate-fraction', isolated from an edible mushroom Astraeus hygrometricus, was evaluated against Leishmania donovani infection both in vitro and in vivo. Ahf-Car induced the expression of inducible nitric oxide synthase 2 (iNOS2) and pro-inflammatory cytokines like TNF-α and I...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12806
更新日期:2020-10-31 00:00:00